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Asexual Mass Propagation of Orchids and its Commercialization: A Review of the Present Status

  • C. J. Goh
Part of the Basic Life Sciences book series (BLSC, volume 22)

Abstract

Orchid is the first horticultural crop successfully mass propagated through tissue culture. It was first shown by Morel (1) in his attempt to produce virus-free Cymbidium from diseased plants. By culturing shoot apices on Knudson III (C) agar medium in small test-tubes, Morel observed that the colorless explant became green and enlarged slowly as a small flat bulblet looking exactly like the protocorm which develops from an embryo. These protocorm-like bodies further differentiated into clumps of protocorms, each developing into a new plant. Later, Morel (2) discovered that the protocorms, when cut into small pieces and subcultured, could regenerate more protocorms, and he estimated it was possible to obtain more than 4,000,000 plants in a year from a single bud. This clonal propagation technique created intense interest among orchid growers and had a tremendous impact on the orchid industry. Many leading orchid growers and nurseries around the world include tissue culture technique as a routine in their operations.

Keywords

Clonal Propagation Callus Tissue Coconut Water Coconut Milk Meristem Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • C. J. Goh
    • 1
  1. 1.Botany DepartmentNational University of SingaporeSingapore

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