Current Studies on Plant Cell Transformation

  • D. Pental
  • E. C. Cocking
Part of the Basic Life Sciences book series (BLSC, volume 22)


As recently discussed (1) a generalized strategy for plant cell transformation requires the construction of a genetic vehicle which is capable either of integrating into the plant genome, or of independently replicating in the plant cell. The minimum attributes of such a vehicle would be the presence of a selectable plant marker and a site for integration of a passenger sequence.


Nitrate Reductase Somatic Hybrid Protoplast Fusion Crown Gall Plant Protoplast 
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  1. 1.
    E.C. Cocking, M.R. Davey, D. Pental and J.B. Power, Aspects of plant genetic manipulation, Nature 293:265 (1981).CrossRefGoogle Scholar
  2. 2.
    J. Draper, M.R. Davey, J.P. Freeman, E.C. Cocking and B.J. Cox, Ti plasmid homologous sequences present in tissues from Agrobacterium plasmid-transformed Petunia protoplasts, Plant Cell Physiol. 23(3):92 (1982).Google Scholar
  3. 3.
    F.A. Krens, L. Molendijk, G.J. Wullems and R.A. Schilperoort, In vitro transformation of plant protoplasts with Ti-plsamid DNA, Nature 298:72 (1982).CrossRefGoogle Scholar
  4. 4.
    M.-D. Chilton, D.A. Tepfer, A. Petit, C. David, F. Casse-Delbart and J. Tempe, Agrobacterium rhizogenes inserts T-DNA into the genomes of the host plant root cells, Nature 295:432 (1982).CrossRefGoogle Scholar
  5. 5.
    E.C. Cocking, Opportunities from the use of protoplasts, Phil. Trans. R. Soc. Lond. B 292:557 (1981).CrossRefGoogle Scholar
  6. 6.
    D.J. Garfinkel, R.B. Simpson, L.W. Ream, F.F. White, M.P. Gordon and E.W. Nester, Genetic analysis of crown gall: fine structure map of the T-DNA by site-directed mutagenesis, Cell 27:143 (1981).PubMedCrossRefGoogle Scholar
  7. 7.
    A.J.M. Matze and M-D. Chilton, Site-specific insertion of genes into T-DNA of the Agrobacterium tumor-inducing plasmid: an approach to genetic engineering of high plant cells, J. of Mol. and Appl. Genetics 1:39 (1981).Google Scholar
  8. 8.
    M.R. Davey, J.P. Freeman, J. Draper and E.C. Cocking, The use of protoplasts for transformation studies with Agrobacterium tumefaciens and its plasmids, in: “Tissue Culture Methods for Plant Pathologists,” D.S. Ingram and J.P. Helgeson, eds., Blackwell, Oxford (1980).Google Scholar
  9. 9.
    D. Pental, S. Cooper-Bland, K. Harding, E.C. Cocking and A.J. Muller, Cultural studies on nitrate reductase deficient Nicotiana tabacum mutant protoplasts, Z. Pflanzenphysiol. 105:219 (1982).Google Scholar
  10. 10.
    A. Kumar, E.C. Cocking, W.A. Bovenberg and A.K. Kool, Restriction endonuclease analysis of chloroplast DNA in interspecies hybrids of Petunia, Theor. and Appl. Genet. (in press) (1982).Google Scholar
  11. 11.
    A. Kumar, D. Wilson and E.C. Cocking, Polypeptide composition of Fraction 1 protein of the somatic hybrid between P. parodii and P. parviflora, Biochem. Genetics 19:255 (1981).CrossRefGoogle Scholar
  12. 12.
    G. Belliard, F. Vedel and G. Pelletier, Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusion, Nature 281:401 (1979).CrossRefGoogle Scholar
  13. 13.
    P. Lebacq and F. Vedel, Sal 1 restriction enzyme analysis of chloroplast and mitochondrial DNAs in the genus Brassica, Plant Sci. Letts. 23:1 (1981).CrossRefGoogle Scholar
  14. 14.
    J.A. Wright, W.H. Lewis, C.L.J. Parfett, Somatic cell genetics: a review of drug resistance, lectin resistance and gene transfer in mammalian cells in culture, Can. J. Genet. and Cytol. 22:443 (1980).Google Scholar
  15. 15.
    H. Lorz, J. Paszhowski, C. Dierks-Ventling and I. Potrykus, Isolation and characterization of cytoplasts and miniprotoplasts derived from protoplasts of cultured cells, Physiologia Plantarum 53:386 (1981).CrossRefGoogle Scholar
  16. 16.
    P.C. Bilkey, M.R. Davey and E.C. Cocking, Isolation, origin and properties of enucleate plant microplasts, Protoplasma 110:147 (1982).CrossRefGoogle Scholar
  17. 17.
    L. Ferenczy, Microbial protoplast fusion, _in: “Genetics as a Tool in Microbiology,” S.W. Glover and D.A. Hopwood, eds., Cambridge University Press, Cambridge, 31st Symposium Society for Exp. Biol. (1981).Google Scholar
  18. 18.
    S. Hasezawar, T. Nagata and K. Syono, Transformation of Vinca protoplasts mediated by Agrobacterium spheroplasts, Mol. Gen. Genet. 182:206 (1981).CrossRefGoogle Scholar
  19. 19.
    W. Schaffner, Direct transfer of cloned genes from bacteria to mammalian cells, Proc. Natl. Acad. Sci. USA 77:2163 (1980).PubMedCrossRefGoogle Scholar
  20. 20.
    R.M. Sandri-Goldin, A.L. Goldin, M. Levine and J.C. Glorioso, High-frequency transfer of cloned herpes simplex virus type 1 sequences to mammalian cells, Mole. and Cell Biology 1:743 (1981).Google Scholar
  21. 21.
    P. Nicholls, Liposomes — as artificial organelles, topochemical matrices, and therapeutic carrier systems, in: “International Review of Cytology,” Suppl. 12:237 (1981).Google Scholar
  22. 22.
    J.F. Makins and G. Holt, Liposome mediated transformation of Streptomyces by chromosomal DNA, Nature 293:671 (1981).PubMedCrossRefGoogle Scholar
  23. 23.
    A. Jiminez and J. Davies, Expression of a transposable antibiotic resistance element in Saccharomyces, Nature 287:869 (1980).CrossRefGoogle Scholar
  24. 24.
    F. Coleber-Garapin, F. Horodniceanu, P. Kourilisky and A.-C. Garapin, A new dormant hybrid selection marker for higher embryotic cells, J. Molec. Biol. 150:1 (1981).CrossRefGoogle Scholar
  25. 25.
    T.-Y. Lu, D. Pental and E.C. Cocking, Plant regeneration from cotyledon protoplasts, Z. Pflanzenphysiol. (in press) (1982).Google Scholar
  26. 26.
    Z.-H. Xu, M.R. Davey and E.C. Cocking, Isolation and sustained division of Phaseolus aureus (Mung bean) root protoplasts, Z. Pflanzenphysiol. 104:289 (1981).Google Scholar
  27. 27.
    M. Wigler, R. Sweet, G.K. Sim, B. Wold, A. Pellicer, E. Lacey, T. Maniatis, S. Silverstein and R. Axel, Transformation of mammalian cells with genes from prokaryotes and eukaryotes, Cell 16:177 (1979).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • D. Pental
    • 1
  • E. C. Cocking
    • 1
  1. 1.Plant Genetic Manipulation Group, Department of BotanyUniversity of NottinghamNottinghamUK

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