Interspecific Somatic Protoplast Fusion Products in Cultivated Jute Species

  • P. M. Kumar
  • K. Das
  • R. R. Sinha
  • P. Mukherjee
  • S. K. Sen
Part of the Basic Life Sciences book series (BLSC, volume 22)


Accounts of success on isolation and culture of plant protoplasts and various genetic manipulation studies including fusion of plant protoplasts from distantly related species have been reported in the past (1, 2, 3). However, records of success amongst major crop plants are not many. Jute is an important fiber crop plant extensively grown in the eastern part of India and Bangladesh. The two cultivated species (Corchorus capsularis and C. olitorius) complement the needs of each other. Production of sexual hybrids is prevented by barriers of sexual incompatibility between the two species. We reported recently (4) that interspecific somatic hybrids may be possible to evolve. On further improving our technical approach, we are convinced that somatic hybrid cell lines have been produced which, however, failed to differentiate into complete plants till date.


Somatic Hybrid Mesophyll Protoplast Isoenzyme Pattern Plant Protoplast Viable Protoplast 


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  1. 1.
    K.N. Kao, F. Constable, M. R. Michayluk and O.L. Gamborg, Plant protoplast fusion and growth of intergeneric hybrid cells, Planta 120:215–227 (1974).CrossRefGoogle Scholar
  2. 2.
    G. Melchers, M.D. Sacristan and A.A. Holder, Somatic hybrid plants of potato and tomato regenerated from fused protoplasts, Carlsberg Res. Commun. 43:203–218 (1978).CrossRefGoogle Scholar
  3. 3.
    Y.Y. Gleba and F. Hoffmann, Hybrid cell lines of Arabidopsis thaliana + Brassica campestris. No evidence for specific chromosome elimination, Molec. Gen. Genet. 165:257–264 (1978).CrossRefGoogle Scholar
  4. 4.
    K. Das, P.M. Kumar and S.K. Sen, Progress in application of protoplast technology in tackling genetical problems of cultivated jute species, in: “Proc. of International Workshop on Improvement of Tropical Crops Through Tissue Culture,” A. S. Islam, ed., Dacca (1981).Google Scholar
  5. 5.
    R.U. Schenk and A.C. Hildebrandt, Medium and techniques for growth of monocotyledonous and dicotyledonous plant cell culture, Can. J. Bot. 50:199–201 (1972).CrossRefGoogle Scholar
  6. 6.
    K. Das, Ph.D. Thesis, Calcutta University (1982).Google Scholar
  7. 7.
    J.B. Power and E.C. Cocking, Isolation of leaf protoplasts. Macromolecule uptake and growth substance response, J. Exp. Bot. 21:64–70 (1970).CrossRefGoogle Scholar
  8. 8.
    P.J. Larkin, Purification and viability determinations of plant protoplasts, Planta 128:213–216 (1976).CrossRefGoogle Scholar
  9. 9.
    I. Potrykus, C.T. Harms and H. Lorz, Callus formation from cell culture protoplasts of corn (Zea mays L.), Theor. Appl. Genet. 54:209–214 (1979).CrossRefGoogle Scholar
  10. 10.
    B.J. Davis, Disc electrophoresis. II. Method and application to serum proteins, Ann. N.Y. Acad. Sci. 121:404–427 (1964).PubMedCrossRefGoogle Scholar
  11. 11.
    H. Binding, Regeneration von haploiden und diploiden pflanzen aus protoplasten von Petunia hybrida L., Z. Pflanzenphysiol. 74:327–356 (1974).CrossRefGoogle Scholar
  12. 12.
    M. Niizeki and F. Kita, Cell division of rice and soybean and their fused protoplasts, Japan J. Breed. 31:161–167 (1981).Google Scholar
  13. 13.
    P. Medgyesy, L. Menczel and P. Maliga, The use of cytoplasmic streptomycin resistance: chloroplast transfer from Nicotiana tabacum into Nicotiana sylvestris and isolation of their somatic hybrids, Mol. Gen. Genet. 179:693–698 (1980).CrossRefGoogle Scholar
  14. 14.
    Nagata and Takebe, Planta 99:12 (1971).CrossRefGoogle Scholar
  15. 15.
    Eriksson, Physiol. Plant. 18:976 (1965).CrossRefGoogle Scholar
  16. 16.
    Frearson et al., Dev. Biol. 33:130 (1973).PubMedCrossRefGoogle Scholar
  17. 17.
    D.F. Blaydes, Interaction of kinetin and various inhibitors in the growth of soybean tissue, Physiol. Plant. 19:738 (1966).CrossRefGoogle Scholar
  18. 18.
    T. Murashige and F. Skoog, A revised medium for rapid growth and bioassays with tobacco tissue culture, Physiol. Plant. 15:473 (1962).CrossRefGoogle Scholar
  19. 19.
    C.C. Chu, C.C. Wang, C.S. Sun, C. Hsu, K.C. Yin and C.Y. Chu, Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources, Scientia Sinica 18:659 (1975).Google Scholar
  20. 20.
    O.L. Gamborg, R.A. Miller and K. Ojima, Nutrient requirements of suspension cultures of soybean root cells, Exp. Cell Res. 50:151 (1968).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • P. M. Kumar
    • 1
  • K. Das
    • 1
  • R. R. Sinha
    • 1
  • P. Mukherjee
    • 1
  • S. K. Sen
    • 1
  1. 1.Programme in Genetical Research, Department of BotanyBose InstituteCalcuttaIndia

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