Protoplast, Cytoplast and Sub-Protoplast from Ripening Tomato Fruits: Their Nature and Fusion Properties
Since the achievement of the fusion of heterotypic protoplasts to bring about somatic hybridization (1), the feasibility of using liposomes (2), vacuoplasts (3) and various types of vesicles (4) for chromosome or organelle transfer is being gradually appreciated. Such protoplast units may be variously produced by differential centrifugation (3) or by synthetic methods (5). The ripening fruits of tomato normally produce various types of protoplasts which may be equally useful (6). The present investigation is addressed to the structure and diversity of these protoplasmic units and their spontaneous and induced fusion properties. The staining reactions with vital basic and acidic dyes also reveal their potential use as marker or fusogen.
KeywordsMethylene Blue Somatic Hybridization Mesophyll Protoplast Fusion Frequency Spontaneous Fusion
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