Tissue Culture Technology for Long-Term Storage and Propagation of Potato (Solanum Tuberosum L.) Germplasms

  • L. E. Heszky
  • K. Enyingi
  • I. Szabo
Part of the Basic Life Sciences book series (BLSC, volume 22)


The in vitro culture of potato (Solanum tuberosum L.) shoot meristems began nearly thirty years ago. During this period the basic techniques and culture conditions have been improved upon. They have been partially suitable for virus elimination. However, they were unsuitable for the in vitro maintenance, long-term storage and vegetative propagation. The induction of multiple shoot development was successful in 1976 (1), but through a callus phase and, hence, had no practical value. With regard to long-term storage satisfactory results in several species of Solanum have been obtained by utilization of meristem and shoot culture techniques (2). In addition cryopreservation methods have also been employed (3, 4). However, until the late seventies: 1) no method was available for increasing the multiplication rate without bringing about some genetical changes; 2) no method was available for unlimited storage without genetical changes; and 3) no culture media were utilizable for a large number of varieties and genetic resources alike without eliminating the genetic and physiological differences.


Indole Acetic Acid Solanum Tuberosum Vegetative Propagation Culture Vessel Indole Acetic Acid 
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  1. 1..
    S. Roest and G.S. Bokelmann, Vegetative propagation of Solanum tuberosum L. in vitro, Potato Res. 19:173–178 (1976).CrossRefGoogle Scholar
  2. 2.
    R. Westcott, G.G. Henshaw and W.M. Roca, Tissue culture storage of potato germplasm: culture initiation and plant regeneration, Plant Sci. Lett. 9:309–315 (1977).CrossRefGoogle Scholar
  3. 3.
    Y.P.S. Bajaj, Technology and prospects of cryopreservation of germplasm, Euphytica 28:267–285 (1979).CrossRefGoogle Scholar
  4. 4.
    Y.P.S. Bajaj, Regeneration of plants from potato meristems freeze-preserved for 24 months, Euphytica 30:141–146 (1981).CrossRefGoogle Scholar
  5. 5.
    M.S. Swaminathan and H.W. Howard, Cytology and genetics of potato (Solanum tuberosum L.) and related species, Bibliographia genetica AG:A–192 (1953).Google Scholar
  6. 6.
    I.R. Denton, R.J. Westcott and B.V. Ford-Lloyd, Phenotypic variation of Solanum tuberosum L. cv. Dr. McIntosh regenerated directly from shoot-tip culture, Potato Res. 20:131–137 (1977).CrossRefGoogle Scholar
  7. 7.
    F.C. Mellor and Stace-Smith, Virus-free potatoes by tissue culture, in: “Applied and Fundamental Aspects of Plant Cell, Tissue and Organ Culture,” J. Reinert and Y.P.S Bajaj, eds., Springer-Verlag, Heidelberg-Berlin-New York (1977).Google Scholar
  8. 8.
    W.M. Roca, N.O. Espinoza, M.R. Roca and J. E. Bryan, A tissue culture method for the rapid propagation of potatoes, American Potato J. 55:691–706 (1978).CrossRefGoogle Scholar
  9. 9.
    W.M. Roca, J.E. Bryan and M.R. Roca, Tissue culture for the international transfer of potato genetic resources, American Potato J. 56:1–11 (1979).CrossRefGoogle Scholar
  10. 10.
    P.B. Goodwin, Y.C. Kim and T. Adisarwanto, Propagation of potato by shoot-tip culture. I. Shoot multiplication, Potato Res. 23:9–18 (1980a).CrossRefGoogle Scholar
  11. 11.
    P.B. Goodwin, Y.C. Kim and T. Adisarwanto, Propagation of potato by shoot-tip culture. II. Rooting of proliferated shoots, Potato Res. 23:19–24 (1980b).CrossRefGoogle Scholar
  12. 12.
    P.B. Goodwin and T. Adisarwanto, Propagation of potato by shoot-tip culture in petri dishes, Potato Res. 23:445–448 (1980c).CrossRefGoogle Scholar
  13. 13.
    P B. Goodwin and G. Brown, Field performance of potato shoot-tips proliferated in culture, Potato Res. 23:449–452 (1980d).CrossRefGoogle Scholar
  14. 14.
    S. Roest and G.S. Bokelmann, In vitro adventitious bud techniques for vegetative propagation and mutation breeding of potato (Solanum tuberosum L.). I. Vegetative propagation in vitro through adventitious shoot formation, Potato Res. 23:167–181 (1980).CrossRefGoogle Scholar
  15. 15.
    F.J. Novak, J. Zadina, V. Horackova and J. Maskova, The effect of growth regulators on meristem tip development and in vitro multiplication of Solanum tuberosum L. plants, Potato Res. 23:155–166 (1980).CrossRefGoogle Scholar
  16. 16.
    K.C. Tao, W.T. Yin and H.Y. Cheng, Meristem culture of potatoes and production of virus-free seed potatoes, in: “Plant Tissue Culture,” Proc. Symp. on Plant Tissue Culture, Science Press, Peking (1978).Google Scholar
  17. 17.
    G. Morel and J.F. Muller, La culture in vitro du meristeme apical de la pomme de terre, Comptes Rendus 258:5250–5252 (1964).Google Scholar
  18. 18.
    T. Murashige and F. Skoog, A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plantarum 15:473–497 (1962).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • L. E. Heszky
    • 1
  • K. Enyingi
    • 2
  • I. Szabo
    • 3
  1. 1.Department of Scientific Research and EducationMinistry for Agriculture and FoodBudapest 55Hungary
  2. 2.Department of Potato BreedingUniversity of AgricultureKeszthelyHungary
  3. 3.Potato Research StationKisvardaHungary

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