Tissue Culture Technology for Long-Term Storage and Propagation of Potato (Solanum Tuberosum L.) Germplasms
The in vitro culture of potato (Solanum tuberosum L.) shoot meristems began nearly thirty years ago. During this period the basic techniques and culture conditions have been improved upon. They have been partially suitable for virus elimination. However, they were unsuitable for the in vitro maintenance, long-term storage and vegetative propagation. The induction of multiple shoot development was successful in 1976 (1), but through a callus phase and, hence, had no practical value. With regard to long-term storage satisfactory results in several species of Solanum have been obtained by utilization of meristem and shoot culture techniques (2). In addition cryopreservation methods have also been employed (3, 4). However, until the late seventies: 1) no method was available for increasing the multiplication rate without bringing about some genetical changes; 2) no method was available for unlimited storage without genetical changes; and 3) no culture media were utilizable for a large number of varieties and genetic resources alike without eliminating the genetic and physiological differences.
KeywordsIndole Acetic Acid Solanum Tuberosum Vegetative Propagation Culture Vessel Indole Acetic Acid
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