Plant Protoplasts: An Experimental System for Cell Biologists

  • L. C. Fowke
  • F. Constabel
Part of the Basic Life Sciences book series (BLSC, volume 22)


Protoplasts are spherical naked plant cells produced by the removal of the cell wall with digestive enzymes (Fig. 1). They are usually derived from either leaf tissue or from cell suspension cultures and have been isolated from a wide variety of plant species. Plant protoplasts can be cultured in defined media and will form a new cell wall, divide, and in many cases regenerate complete plants (e.g. 1, 2, 3, 4). Protoplasts provide a unique experimental system for studies of the structure and function of plant cells. The removal of the cell wall from plant cells, for example, has created new and exciting possibilities for exploring the structure, chemistry and function of the plant plasma membrane. Some of the more important applications in cell biology are listed in Table 1. This table includes only recent selected references and, therefore, does not contain a comprehensive list of relevant literature.


Protoplast Fusion Ergot Alkaloid Cell Wall Formation Cytoplasmic Organelle Membrane Fragment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • L. C. Fowke
    • 1
  • F. Constabel
    • 2
  1. 1.Department of BiologyUniversity of SaskatchewanSaskatoonCanada
  2. 2.National Research Council of CanadaPrairie Regional LaboratorySaskatoonCanada

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