Development of Zygotic Barley Embryos In Vitro

  • J. M. Dunwell
Part of the Basic Life Sciences book series (BLSC, volume 22)


Both mature and immature embryos have frequently been used as sources of material for studies of callus initiation and regeneration in cereals (1). Although they have proved to be the explants most amenable to culture, the substantial inter- and intraspecific differences in response remain an obstacle to any application of cell culture methods to variety improvement. As part of a continuing investigation into this problem a previous study conducted on three contrasting barley genotypes examined the influence of incubation temperature and medium composition on the growth of mature embryos in vitro (2), These results were discussed in relation to the known dormancy characteristics of some of these genotypes (1). It was found that there was a significant difference in the response of these genotypes to particular combinations of 2,4-D and sucrose present in the culture medium.


Culture Period Shoot Length Sucrose Concentration Immature Embryo Mature Embryo 
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Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • J. M. Dunwell
    • 1
  1. 1.John Innes InstituteNorwichUK

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