In Vitro Adjuvant Effect of Endotoxin
Mishell and Dutton (1967) devised a method that initiates an immune response in cultures of free spleen cells from unimmunized mice. This method allows the investigation of biochemical and cellular events associated with induction and development of the immune response and enables the investigator to use an intrinsically simpler system for analysis than is true when intact animals are employed.
KeywordsBone Marrow Cell Spleen Cell Adjuvant Effect Adjuvant Action Bone Marrow Cell Culture
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- Kishimoto, S., Takahama, T., and Mizumachi, H., 1976, In vitro immune response to the 2,4,6-trinitrophenyl determinant in aged G57BL/6J mice: Changes in the humoral immune response to, avidity for the TNP determinant and responsiveness to LPS effect with aging, J. Immunol. 116:294.PubMedGoogle Scholar
- McGhee, J. R., Farrar, J.J., Michalek, S. M., Mergenhagen, S. E., and Rosenstreich, D. L., 1979, Cellular requirements for lipopolysaccharide adjuvanticity: A role for both T lymphocytes and macrophages for in vitro responses to particulate antigens, J. Exp. Med. 149:793.PubMedCrossRefGoogle Scholar
- Persson, U., 1977, Lipopolysaccharide-induced suppression of the primary immune response to a thymus-dependent antigen, J. Immunol. 118:789.Google Scholar