Abstract
A perusal of the literature on endotoxins in the past decade makes it clear that a renewed interest has developed to explain the multiple pathophysiological and immunological host responses to lipopolysac-charide endotoxin (LPS). In great measure this was due to the recognition that many of these responses were under genetic control. The genetic approach taken by numerous workers arose from the discovery of the mutant C3H/HeJ mouse strain, which is highly resistant to the toxic effects of LPS and which displays cellular responses both in vivo and in vitro that are diametrically opposite to those from normally susceptible mice (Sultzer, 1968, 1969). The availability of this inbred strain gave rise to a variety of comparative studies so that a rather extensive literature on this subject has accumulated and continues to do so. However, within the allotted space for this article, I will only attempt to highlight those aspects of the work with this strain that have dealt with the activation of lymphocytes by LPS, and to discuss in some detail the newer studies originating from the use of the C3H/HeJ mouse that have established that various proteins associated with the LPS in the outer membrane of gram-negative bacteria are potent activators of immunocompetent cells.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Andersson, J., Möller, G., and Sjöbcrg, O., 1972a, Selective induction of DNA synthesis in T and B lymphocytes, Cell. Immunol. 4:381.
Andersson, J., Sjöberg, O., and Möller, G., 1972b, Induction of immunoglobulin and antibody synthesis in vitro by lipopolysaccharides, Eur. J. Immunol. 2:349.
Andersson, J., Coutinho, A., and Melchers, F., 1977, Frequencies of mitogen-reactive B cells in the mouse. I. Distribution in different lymphoid organs from different inbred strains of mice at different ages, J Exp. Med. 145:1511.
Betz, S. J., and Morrison, D. C., 1977, Chemical and biological properties of a protein rich fraction of bacterial lipopolysaccharides. I. The in vitro murine lymphocyte response, J. Immunol. 119:1475.
Bick, P. H., Persson, U., Smith, E., Möller, G., and Hammarström, L., 1977, Genetic control of lymphocyte activation: Lack of response to low doses of concanavalin A in lipopolysaccharide-nonresponder mice, J. Exp. Med. 146:1146.
Braley-Mullen, H., Hayes, N., and Sanders, M., 1977, Suppression of IgM responses to type III pneumococcal polysaccharide by lipopolysaccharide (LPS), Cell. Immunol. 30: 300.
Braun, V., 1977, Lipoprotein from the outer membrane of Escherichia coli as an antigen, immunogen, and mitogen, in: Microbiology 1977 (D. Schlessinger, ed.), pp. 251–261, American Society for Microbiology, Washington, D.C.
Chedid, L., and LeGarrec, Y., 1980, Transfer of cells or of serum factors in nonspecific resistance to infection, in: Microbiology 1980 (D. Schlessinger, ed.), pp. 19–24, American Society for Microbiology, Washington, D.C.
Coutinho, A., 1976, Genetic control of B-cell responses. II. Identification of the spleen B-cell defect in C3H/HeJ mice, Scand. J. Immunol. 5:129.
Coutinho, A., and Meo, T., 1978, Genetic basis for unresponsiveness to lipopolysaccha-ride in C57B1/10/Cr mice, Immunogenetics 7:17–24.
Coutinho, A., Forni, L., and Watanabe, T., 1978, Genetic and functional characterization of an antiserum to the lipid-A-specific triggering receptor on murine B-cells, Eur. J. Immunol. 8:63.
Eisenstein, T. K., and Angerman, C. R., 1978, Studies on the protective capacity and immunogenicity of lipopolysaccharide, acetone-killed cells, and a ribosome-rich extract of Salmonella typhimunum in C3H/HeJ and CD-I mice, J. Immunol. 121:1010.
Eisenstein, T. K., and Sultzer, B. M., 1981, Salmonella vaccines: Protection by endotoxin protein and lipopolysaccharide in two different mouse strains, in: Bacterial Vaccines, Vol. 4.(J. Robbins, J. Hill, and J. Sadoff, eds.), pp. 423–128, Thieme-Stratton, New York.
Eisenstein, T. K., Deakins, L. W., and Sultzer, B. M., 1980, The C3HeB/FeJ mouse, a strain in the C3H lineage which separates Salmonella susceptibility and immunizability from mitogenic responsiveness to lipopolysaccharide, in: Genetic Control of Natural Resistance to Infection and Malignancy (E. Shamere, P. A. R. Kongsharer, and M. Landy, eds.), pp. 115–120, Academic Press, New York.
Esquivei, P. S., Kong, Y. M., and Rose, N. R., 1978, Evidence for thyroglobulin-reactive T cells in good responder mice, Cell. Immunol. 37:14.
Forni, L., and Coutinho, A., 1978, An antiserum which recognizes lipopolysaccharide-re-active B cells in the mouse, Eur. J. Immunol. 8:56.
Gery, I., Kruger, J., and Spiesel, S. Z., 1972, Stimulation of B-lymphocytes by endotoxin: Reactions of thymus-deprived mice and karyotypic analysis of dividing cells in mice bearing T6T6 thymus grafts, J Immunol. 108:1088.
Glode, L. M., and Rosenstreich, D. L., 1976, Genetic control of B cell activation by bacterial lipopolysaccharide is mediated by multiple distinct genes or alleles, J. Immunol. 117:2061.
Glode, L. M., Mergenhagen, S. E., and Rosenstreich, D. L., 1976, Significant contribution of spleen cells in meditating the lethal effects of endotoxin in vivo, Infect. Immun. 14:626.
Goodman, G. W., and Sultzer, B. M., 1979a, Further studies on the activation of lymphocytes by endotoxin protein, J. Immunol. 122:1329.
Goodman, G. W., and Sultzer, B. M., 1979b, Endotoxin protein is a mitogen and polyclonal activator of human B-lymphocytes, J. Exp. Med. 149:713.
Goodman, G. W., and Sultzer, B. M., 1979c, Characterization of the chemical and physical properties of a novel B-lymphocyte activator, endotoxin protein, Infect. Immun. 24:685.
Goodman, M. G., and Weigle, W. O., 1979, T-Cell regulation of polyclonal B-cell responses. I. Helper effects of T-cells, J Immunol. 122:2548.
Goodman, M. G., Parks, D. E., and Weigle, W. O., 1978, Immunologic responsiveness of the C3H/HeJ mouse: Differential ability of the butanol-extracted lipopolysaccharide (LPS) to evoke LPS-mediated effects, J Exp. Med. 147:800.
Gregory, S. H., Zimmerman, D. H., and Kern, M., 1980, The lipid A moiety of lipopolysaccharide is specifically bound to B-cell subpopulations of responder and nonresponder animals, J Immunol. 125:102.
Hämmerling, I., Chin, A. F., Abbot, J., and Scheid, M. C., 1975, The ontogeny of murine B-lymphocytes. I. Induction of phenotypic conversion of la to Ia+ lymphocytes, J. Immunol. 115:1425.
Hoffman, M. K., Galanos, C., Koenig, S., and Oettgen, H. F., 1977, B-Cell activation by lipopolysaccharide: Distinct pathways for induction of mitosis and antibody production, J. Exp. Med. 146:1640.
Kabir, S., and Rosenstreich, D. L., 1977, Binding of bacterial endotoxin to murine spleen lymphocytes, Infect. Immun. 15:156.
Kong, Y. M., El-Rehewy, M., Giraldo, A. A., Esquivei, P. S., Jeffries, C. D., Rose, N. R., and David, C. S., 1978, The effect of LPS responsiveness on induction of autoimmune thyroiditis, Fed. Proc. 37:1380.
Kreisler, J. M., and Möller, G., 1974, Effect of PPD on the specific immune response to heterologous red cells in vitro, J. Immunol. 112:151.
Kuusi, N., Nurminen, M., Saxen, H., Valtonen, M., and Makela, P. H., 1979, Immunization with major outer membrane proteins in experimental salmonellosis of mice, Infect. Immun. 25:857.
Levy, G. A., and Edgington, T. S., 1980, Lymphoid procoagulant activity and mitogenesis in the G3H/HeJ mouse: Discordant response to lipopolysaccharide stimulation, J. Immunol. 124:2665.
Männel, D. N., Rosenstreich, D. L., and Mergenhagen, S. E., 1979, Mechanism of lipo-polysaccharidc-induced tumor necrosis: Requirement for lipopolysaccharide sensitive lymphoreticular cells, Infect. Immun. 24:573.
Melchers, F., 1977, B lymphocyte development in fetal liver. I. Development of reactivities to B-cell mitogens in vivo and in vitro, Eur. J. Immunol. 7:476.
Morrison, D. C., and Betz, S. J., 1977, Ghemical and biological properties of a protein rich fraction of bacterial lipopolysaccharide. II. The in vitro peritoneal mast cell response, J. Immunol. 119:1790.
Morrison, D. C., and Leive, L. 1975, Fractions of lipopolysaccharide from Escherichia coli Ol 11:B4 prepared by two extraction procedures, J. Biol. Chem. 250:2911.
Morrison, D. C., and Ryan, J. L., 1979, Bacterial endotoxins and host immune responses, Adv. Immunol. 28:294.
Morrison, D. C., Betz, S. J., and Jacobs, D. M., 1976, Isolation of a lipid A bound polypeptide responsible for “LPS-initiated” mitogenesis of C3H/HeJ spleen cells, J. Exp. Med. 144:840.
Morrison, D. C., Wilson, M. E., Razuiddin, S., Betz, S. J., Gurry, B. J., Dades, Z., and Munkenbeck, P., 1980, Influence of lipid A-associated protein on endotoxin stimulation of nonlymphoid cells, in: Microbiology 1980 (D. Schlessinger, ed.), pp. 30–35, American Society for Microbiology, Washington, D.C.
Nilsson, B. S., Sultzer, B. M., and Bullock, W. W., 1973, Purified protein derivative of tuberculin induces immunoglobulin production in normal mouse spleen cells, J. Exp. Med. 137:127.
Norcross, M. A., and Smith, R. T., 1977, Regulation of B-cell proliferative responses to lipopolysaccharide by a subclass of thymus T-cells, J. Exp. Med. 145:1299.
Nygren, H., Dahlen, G., and Möller, G., 1979, Bacterial lipopolysaccharides bind selectively to lymphocytes from lipopolysaccharide high-responder mouse strains, Scand. J. Immunol. 10:555.
Peavy, D. L., Adler, W. H., and Smith, R. T., 1970, The mitogenic effects of endotoxin and staphylococcal enterotoxin B on mouse spleen cells and human peripheral lymphocytes, J. Immunol. 105:1453.
Primi, D., Hammarström, L., Möller, G., Smith, C. I. E., and Uhr, J., 1979, Gon-A-activated T-cells scerete factors with polyclonal B-cell-activating properties, Scand. J. Immunol. 9:467.
Rosenstreich, D. L., and McAdam, K. P. W. J., 1979, Lymphoid cells in endotoxin-induced production of the amyloid-related serum amyloid A protein, Inject. Immun. 23: 181.
Rosenstreich, D. L., Glode, L. M., Wahl, L. M., Sandberg, A. L., and Mcrgenhagen, S. E., 1977, Analysis of the cellular defects of endotoxin-unresponsive C3H/HeJ mice, in: Microbiology 1977 (D. Schlessinger, ed.), pp. 314–320, American Society for Microbiology, Washington, D.C.
Rudbach, J. A., and Reed, N. D., 1977, Immunological responses of mice to lipopolysac-charide: Lack of secondary responsiveness by C3H/HeJ mice, Infect. Immun. 16:513.
Skidmore, B. J., Morrison, D. C., Chiller, J. M., and Weigle, W. O., 1975, Immunologic properties of bacterial lipopolysaccharide (LPS). II. The unresponsiveness of C3H/HeJ mouse spleen cells to LPS-induced mitogenesis is dependent on the method used to extract LPS, J. Exp. Med. 142:1488.
Skidmore, B. J., Chiller, J. M., and Weigle, W. O., 1977, Immunologic properties of bacterial lipopolysaccharide (LPS). IV. Cellular basis of the unresponsiveness of C3H/HeJ mouse spleen cells to LPS-induced mitogenesis, J. Immunol. 118:274.
Slowe, A., and Waldmann, H., 1975, The “intrinsic adjuvanticity” and immunogenicity of trinitrophenylated lipopolysaccharide, Immunology 29:825.
Sultzer, B. M., 1968, Genetic control of leucocyte responses to endotoxin, Nature (London) 219:1253.
Sultzer, B. M., 1969, Genetic factors in leucocyte responses to endotoxin: Further studies in mice, J. Immunol. 103:32.
Sultzer, B. M., 1972, Genetic control of host responses to endotoxin, Infect. Immun. 5:107.
Sultzer, B. M., 1973, Mitogenic and immune responses to endotoxin: A deficiency in C3H/HeJ mice, Abstr. Annu. Meet. Am. Soc. Microbiol. p. 85, M69.
Sultzer, B. M., 1976, Genetic analysis oflymphocyte activation by lipopolysaccharide endotoxin, Infect. Immun. 13:1579.
Sultzer, B. M., and Goodman, G. W., 1976, Endotoxin protein: A B-cell mitogen and polyclonal activator of C3H/HeJ lymphocytes, J. Exp. Med. 144:821.
Sultzer, B. M., and Nilsson, B. S., 1972, PPD-tuberculin—A B-cell mitogen, Sature Sew Biol. 240:198.
Sultzer, B. M., Nilsson, B. S., and Kirschenbaum, D., 1977, Nonspecific stimulation of lymphocytes by tuberculin, Inject. Immun. 15:799.
Sultzer, B. M., Goodman, G. W., and Eisenstein, T. K., 1980, Endotoxin protein as an immunostimulant, in: Microbiology 1980 (D. Schlessinger, ed.), pp. 61–65, American Society for Microbiology, Washington, D.C.
Symons, D. B. A., and Clarkson, C. A., 1979, The binding of LPS to the lymphocyte surface, Immunology 38:503.
Tanabe, M. J., and Nakano, M., 1979a, Dysfunction of thymocytes of C3H/HeJ mice in blastogenic response to antithymocyte serum in vitro and its repair with lipopolysaccharide induced mediator, Microbiol. Immunol. 23:287.
Tanabe, M. J., and Nakano, M., 1979b, Lipopolysaccharide-induced mediators assisting the proliferative response of C3H/HeJ thymocytes to concanavalin A, Microbiol. Immunol. 23:1097.
Vallera, D. A., Gamble, C. E., and Schmidtke, J. R., 1980, Lipopolysaccharide-induced immunomodulation of the generation cell-mediated cytoxicity. II. Evidence for the involvement of a regulatory B lymphocyte, J. Immunol. 124:641.
Watanabe, T., and Ohara, J., 1981, Functional nuclei of LPS-nonrespondcr C3H/HeJ mice after transfer into LPS-responder C3H/HeN cells by cell fusion, Nature (London) 290:58.
Watson, J., 1977, Differentiation of B lymphocytes in C3H/HeJ mice: The induction of la antigens by lipopolysaccharide, J. Immunol. 118:1103.
Watson, J., and Riblet, R., 1974, Genetic control of responses to bacterial lipopolysaccharide in mice. I. Evidence for a single gene that influences mitogenic and immunogenic responses to lipopolysaccharides, J. Exp. Med. 140:1147.
Watson, J., and Riblet, R., 1975, Genetic control of responses to bacterial lipopoly-saccharides in mice. II. A gene that influences the activity of a membrane receptor for lipopolysaccharides, J. Immunol. 114:1462.
Watson, J., Riblet, R., and Taylor, B., 1977, The response to lipopolysaccharides in recombinant inbred lines of mice, J Immunol. 118:1604.
Watson, J., Kelly, K., Largen, M., and Taylor, B. A., 1978a, The genetic mapping of defective LPS response gene in C3H/HeJ mice, J. Immunol. 120:422.
Watson, J., Largen, M., McAdam, K. P. W. J., and Taylor, B. A., 1978b, Genetic control of endotoxic responses in mice, J. Exp. Med. 147:39.
Watson, J., Kelly, K., and Whitlock, C., 1980, Genetic control of endotoxin sensitivity, in: Microbiology 1980 (D. Schlessinger, ed.), pp. 4–10, American Society for Microbiology, Washington, D.C.
Yoshida, T., Hidekichi, S., and Cohen, S., 1973, The protection of migration inhibition factor by B and T cells of the guinea pig, J. Exp. Med. 38:784.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1983 Plenum Press, New York
About this chapter
Cite this chapter
Sultzer, B.M. (1983). Lymphocyte Activation by Endotoxin and Endotoxin Protein. In: Nowotny, A. (eds) Beneficial Effects of Endotoxins. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4364-6_11
Download citation
DOI: https://doi.org/10.1007/978-1-4684-4364-6_11
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-4366-0
Online ISBN: 978-1-4684-4364-6
eBook Packages: Springer Book Archive