The Estimation of DNA by Absorption Cytophotometry on Smears of Trans-Rectal Fine Needle Aspiration of the Prostate

  • C. R. Bouffioux
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 53)


Mitosis, the predominant step of cell multiplication, occurs when a cell has doubled its metabolic and genetic contents.


Prostatic Cancer Chronic Prostatitis Aneuploid Cell Feulgen Reaction Prostatic Adenoma 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    R. Bassleer, Recherches sur les protéines nucléaires totales et les acides désoxyribonucléiques dans les fibroblastes cultives in vitro et dans les cellules tumorales d’Ehrlich. Une étude cytochimique quantitative réalisée par micro-interférométrie et cytophotométrie. (Thèse d’agrégation de l’Enseignement Supérieur). Arch. Biol. 79:2 181–325 (1968).Google Scholar
  2. 2.
    I. Cameron, Is the duration of DNA synthesis in somatic cells of mammals and birds a constant? J. Cell. Biol. 20:185–187 (1964).PubMedGoogle Scholar
  3. 3.
    L. Adams, S. Dahlgren, Cytophotométric measurements of the DNA content of lung tumours, Acta Path. Microbiol. Scand., 72:561–574 (1968).PubMedCrossRefGoogle Scholar
  4. 4.
    N. Atkin, Nuclear size in carcinoma of the cervix; its relation to DNA content and prognosis, Cancer 17:1391–1394 (1964).PubMedCrossRefGoogle Scholar
  5. 5.
    N. Inui, and K. Oota, DNA content of human tumor cell nucleus. A study on gastric carcinoma, with special reference to its histological features, Gann 56:567–574 (1965).PubMedGoogle Scholar
  6. 6.
    C. Leuchtenberg, R. Leuchtenberg and A. Davis, A microspectre— photometric study of the desoxyribose nucleic acid (DNA) content in cells of normal and malignant human tissues, Am. J. Path. 30:65–85 (1954).Google Scholar
  7. 7.
    W. Sandritter, M. Carl and W. Ritter, Cytophotometric measurements of the DNA content of human malignant tumors by means of Feulgen reaction, Acta Cytol. 10:26–30 (1966).PubMedGoogle Scholar
  8. 8.
    R. Feulgen and H. Rossenbeck, Mikroskopisch-chemischer Nachweis einer Nukleinsaüre vom Typus der Thymokleinsaüre und die darauf berukende elektive färbung von Zelkernen in microskopischen Präparten. Hoope Seylers. Z. Phys. Chem. 135:203–248 (1924).CrossRefGoogle Scholar
  9. 9.
    F. Kasten, The Feulgen reaction. An enigma in cytochemistry, Acta Histochem. 17:88–99 (1964).PubMedGoogle Scholar
  10. 10.
    A. Zetterberg and P. Esposti, Cytophotometric DNA analysis of aspirated cells from prostatic carcinoma, Cytol.(Baltimore) 20:46 (1976).Google Scholar
  11. 11.
    C. Bouffioux, Le cancer de la prostate, Acta Urol. Belg. 47, V. 344–348 (1979).Google Scholar
  12. 12.
    L. Persky and C. Leuchtenberger, Cytochemical studies of prostatic epithelium: I. The desoxyribose nucleic (DNA) content in individual cells, J. Urol.78:788–795 (1957).PubMedGoogle Scholar
  13. 13.
    E. Sprenger, L. Volk and W. Michaelis, Die Aussagekraft der Zelkern-DNS-Bestimmung bei der Diagnostik des Prostatakarzinoms, Beitr. Path.Bd. 153:370–378 (1974).CrossRefGoogle Scholar
  14. 14.
    P. Bichel, P. Frederiksen, T. Kjaer, P. Thommesen and L. Vindeløv, Flow micro-fluorometry and transrectal fine needle biopsy in the classfication of human prostatic carcinoma, Cancer 40:1206–1211 (1977).PubMedCrossRefGoogle Scholar
  15. 15.
    A. Tavares, J. Costa, A. de Carvalho and M. Reis, Tumour polyploidy and prognosis in carcinomas of the bladder and prostate, Brit. J. Cancer 20:438–441 (1966).PubMedCrossRefGoogle Scholar
  16. 16.
    T. Kjaer, P. Thommesen, P. Frederiksen and P. Bichel, DNA content in cells aspirated from carcinoma of the prostate treated with oestrogenic compounds, Urol. Res. 7:249–251 (1979).PubMedCrossRefGoogle Scholar
  17. 17.
    W. Leistenschneider and R. Nagel, Estracyt therapy of advanced prostatic cancer with special reference to control of therapy with cytology and DNA cytophotometry, Eur. Urol. 6:11–115 (1980).Google Scholar
  18. 18.
    L. Vindeløv, Flow microfluorometric analysis of nuclear DNA in cells from solid tumors and cell suspensions. A new method for rapid isolation and staining of nuclei. Virchows Arch. B. Cell Path. 24:227–242 (1977).Google Scholar
  19. 19.
    A. Zimmerman and F. Truss, Flow-through-Cytophotometry, Urol. Res. 7:1–3 (1979).CrossRefGoogle Scholar
  20. 20.
    B. Tribukait, P.L. Esposti and L. Rönström, Tumour ploidy for characterization of prostatic carcinoma: Flow cyto-fluoro-metric DNA studies using aspiration biopsy material, Scand. J. Urol. Nephrol., Suppl. 55:59–64 (1980)Google Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • C. R. Bouffioux
    • 1
  1. 1.Department of UrologyUniversity of LiègeBelgium

Personalised recommendations