Epithelium and Stroma in Prostatic Cancer and Hyperplasia

  • M. A. Blankenstein
  • J. C. Romijn
  • G. J. van Steenbrugge
  • F. H. Schröder
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 53)


The normal morphogenesis and cytodifferentiation of rodent prostatic epithelium is strongly dependent on the continuous association with mesenchymal cells of proper (i.e. urogenital) origin (1,2). The inductive capacity of urogenital stroma has been tested on different epithelia, and it was found that, in the presence of androgens, integumental epithelium was transformed by urogenital stroma into a glandular epithelium characteristic of the source of the stroma (1,3). Moreover, adult bladder epithelium could be induced to form prostate-like acini by embryonic stroma of urogenital origin (3)- From these observations McNeal (4) hypothesized that the formation of prostatic acini during the development of benign prostatic hyperplasia may be the result of a re-expression of embryonic inductive capacity. Cunha et al. (5) summarized the prerequisites for prostatic development to be i) the presence of androgens; ii) the presence of “inductive” stroma and iii) the ability of the epithelium to respond to the inductive influences.


Androgen Receptor Benign Prostatic Hyperplasia Prostatic Carcinoma Prostatic Acid Phosphatase Prostatic Epithelium 


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  1. 1.
    G.R. Cunha, Tissue interactions between epithelium and mesenchyme of urogenital and integumental origin, Anat. Rec. 172:529 (1972).PubMedCrossRefGoogle Scholar
  2. 2.
    I. Lasnitzki and T. Mizuno, Induction of the rat prostate gland by androgens in organ culture, J.Endocr. 74:47 (1977).PubMedCrossRefGoogle Scholar
  3. 3.
    G.R. Cunha and B. Lung, The importance of stroma in morphogenesis and functional activity of urogenital epithelium, In vitro 15:50 (1978).CrossRefGoogle Scholar
  4. 4.
    J.E. McNeal, Origin and evolution of benign prostatic enlargement, Invest.Urol. 15:340 (1978).PubMedGoogle Scholar
  5. 5.
    G.R. Cunha, B. Lung and B. Reese, Glandular epithelial induction by embryonic mesenchyme in adult bladder epithelium of Balb/c mice, Invest.Urol. 17:302 (1980).PubMedGoogle Scholar
  6. 6.
    W.I.P. Mainwaring, “The Mechanism of Action of Androgens,” Springer-Verlag, New York (1977).Google Scholar
  7. 7.
    N. Bruchovsky and J.D. Wilson, The conversion of testosterone to 5α-androstan-17β-ol-3-one by rat prostate in vivo and in vitro, J.Biol.Chem. 243:2012 (1968).PubMedGoogle Scholar
  8. 8.
    M. Menon, C.E. Tananis, M.G. McLoughlin, and P.C. Walsh, Androgen receptors in human prostatic tissue-review, Cancer Treatment Rep. 61:265 (1977).Google Scholar
  9. 9.
    L.L. Hicks and P.C. Walsh, A microassay for the measurement of androgen receptors in human prostatic tissue, Steroids 33:389 (1979).PubMedCrossRefGoogle Scholar
  10. 10.
    M. Snochowski, A. Pousette, P. Ekman, D. Bression, L. Andersson, B. Hogberg, and J.A. Gustafsson, Characterisation and measurement of androgen receptor in human benign prostatic hyperplasia and prostatic carcinoma, J.Clin.Endocr.Metab. 45:920 (1977).PubMedCrossRefGoogle Scholar
  11. 11.
    D.A.N. Sirett, S.K. Cowan, A.E. Janeczo, J.K. Grant, and E.S. Glen, Prostatic tissue distribution of 17β-hydroxy-5α-andros-tan-3-one and of androgen receptors in benign hyperplasia, J.Steroid Biochem. 13:723 (1980).PubMedCrossRefGoogle Scholar
  12. 12.
    P.K. Siiteri and J.D. Wilson, Dihydrotestosterone in prostatic hypertrophy, J.clin.Invest. 49:1737 (1970).PubMedCrossRefGoogle Scholar
  13. 13.
    R.F. Morfin, S. DiStefano, J.P. Bercovici, and H.H. Floch, Comparison of testosterone, 5α-dihydrotestosterone and 5 -androstane-3β, 17p-diol metabolisms in human normal and hyperplastic prostates, J.Steroid Biochem. 9:245 (1978).PubMedCrossRefGoogle Scholar
  14. 14.
    J.C. Romijn, K. Oishi, J. Bolt-de Vries, H.U. Schweikert, E. Mulder, and F.H. Schröder, Androgen metabolism and androgen receptors in separated epithelium and stroma of the human prostate, in:”Steroid Receptors, Metabolism and Prostatic Cancer,” F.H. Schröder and H.J. de Voogt, eds., Exerpta Medica, Amsterdam, (1980) p. 134.Google Scholar
  15. 15.
    L.M. Franks, P.N. Riddle, A.W. Carboneil, and G.O. Gey, A comparative study of the ultrastructure and lack of growth capacity of adult human prostate epithelium mechanically separated from its stroma, J.Pathol. 100:113 (1970).PubMedCrossRefGoogle Scholar
  16. 16.
    R.A. Cowan, S.K. Cowan, C.A. Giles, and J.K. Grant, Prostatic distribution of sex hormone-binding globulin and cortisol-binding globulin in benign hyperplasia, J.Endocr. 71:121 (1976).PubMedCrossRefGoogle Scholar
  17. 17.
    S.R. Helms, R.I. Brazeal, A.J. Bueschen, and T.G. Pretlow, Separation of cells with histochemically demonstrable acid phosphatase activity from suspensions of human prostatic cells, Am.J.Pathol. 80:79 (1975).PubMedGoogle Scholar
  18. 18.
    T.G. Pretlow, M.G. Brattain, and J.I. Kreisberg, Separation and characterization of epithelial cells from prostates and prostatic carcinomas: a review, Cancer Treatment Rep. 61:157 (1977).Google Scholar
  19. 19.
    K. Oishi, J.C. Romijn, and F.H. Schröder, Cell separation and characterization of epithelial cells from human benign prostatic hyperplasia. The prostate, In press, (1981).Google Scholar
  20. 20.
    M.M. Webber, Normal and benign human prostatic epithelium in culture. I. Isolation. In vitro 15:967 (1979).PubMedCrossRefGoogle Scholar
  21. 21.
    J.F. Lechner, M.S. Babcock, M. Marnell, K.S. Narayau, and M.E. Kaighn, Normal human prostate epithelial cell cultures, Methods in cell biology 21B:195 (1980).PubMedCrossRefGoogle Scholar
  22. 22.
    D.S. Coffey, The biochemistry and physiology of the prostate and seminal vesicles, in:”Campbells Urology,” 4th edition, H. Harrison, R.F. Gittes, A.D. Perlmutter, T.A Stamey, and P.C. Walsh, eds., Saunders, Philadelphia, (1978), Vol.1, p. 161.Google Scholar
  23. 23.
    J.A. Serrano, W.A. Shannon, N.J. Sternberger, H.L. Wasserkrug, A.A. Serrano, and A.A. Seligman, The cytochemical determination of prostatic acid phosphatase using a new substrate:phosphoryl-choline, J.Histochem Cytochem. 24:1046 (1976).PubMedCrossRefGoogle Scholar
  24. 24.
    A.C. Jöbsis, G.P. de Vries, R.R.H. Anholt, and G.T.B. Sanders, Demonstration of the prostatic origin of metastases. An immunohistochemical method for formalin-fixed embedded tissue, Cancer 41:1788 (1978).PubMedCrossRefGoogle Scholar
  25. 25.
    H.R. Herschman, Prostatic acid phosphatase and cancer, T.I.B.S. 5:82 (1980).Google Scholar
  26. 26.
    R.W. Ban, J.F. Cooper, H. Imfeld, and A. Foti, Hormonal effects on prostatic acid phosphatase synthesis in tissue culture, Invest.Urol. 11:308 (1974).PubMedGoogle Scholar
  27. 27.
    M.M. Webber, In vitro models for prostatic cancer, in: ”Models for Prostatic Cancer,” G.P. Murphy, ed., Progr.clin.biol.Res. 37, Alan R. Liss, New York, (1980), p. 133.Google Scholar
  28. 28.
    P. Chaisiri, M.E. Harper, R.W. Blaney, W.P. Peeling, and K. Griffiths, Plasma spermidine concentrations in patients with tumours of the breast or prostate or testis, Clin.Chem. Acta 104:367 (1980).CrossRefGoogle Scholar
  29. 29.
    R.D. Williams, D.L. Bronson, A.Y. Elliott, G.W. Gehrke, K. Kuob, and E.E. Fraley, Biochemical markers of cultured human prostatic epithelium, J.Urol. 119:768 (1978).PubMedGoogle Scholar
  30. 30.
    J.C. Romijn, K. Oishi, and F.H. Schröder, Investigations on separated epithelium and stroma of human prostatic tissue, The Prostate 1:118 (1980).Google Scholar
  31. 31.
    M. Krieg, G. Klötzl, J. Kaufmann, and K.D. Voigt, Stroma of human benign prostatic hyperplasia: preferential tissue for androgen metabolism and oestrogen binding, Acta endocr. 96:422 (1981).PubMedGoogle Scholar
  32. 32.
    F.K. Habib, A.L. Tesdale, G.D. Chisholm, and A. Busuttil, Prostatic retropubic and transurethral specimens. A comparison of androgen metabolism in the respective stromal and epithelial components, The Prostate 1:117 (1980).Google Scholar
  33. 33.
    N. Bruchovsky and G. Lieskovsky, Increased ratio of 5α-reductase: 3α(B)-hydroxysteroid dehydrogenase activities in the hyperplastic human prostate, J.Endocr. 80:289 (1979).PubMedCrossRefGoogle Scholar
  34. 34.
    R.P. Wilkin, N. Bruchovsky, T.K. Shnitka, P.S. Rennie, and T.L. Comeau, Stromal 5α-reductase activity is elevated in benign prostatic hyperplasia, Acta endocr. 94:284 (1980).PubMedGoogle Scholar
  35. 35.
    H.U. Schweikert, H.J. Hein, J.C. Romijn, and F.H. Schröder, Testosterone metabolism of fibroblasts grown from prostatic carcinoma, benign prostatic hyperplasia and skin fibroblasts. Invest, Urol. In Press (1981).Google Scholar
  36. 36.
    M. Krieg, I. Grobe, K.D. Voigt, E. Altenähr, and H. Klosterhalfen, Human prostatic carcinoma: significant differences in its androgen binding and metabolism compared to the human benign prostatic hypertrophy, Acta endocr. 88:397 (1978)PubMedGoogle Scholar
  37. 37.
    L.P. Pertschuk, E.H. Tobin, P. Tanapat, E. Gaetjens, A.C. Carter, N.D. Bloom, R.J. Macchia, and K.B. Eisenberg, Histochemical analysis of steroid hormone receptors in breast and prostatic carcinoma, J. Histochem. Cytochem. 28:799 (1980).PubMedCrossRefGoogle Scholar
  38. 38.
    L.P. Pertschuk, D.T. Zava, E.H. Tobin, D.J. Brigati, E. Gaetjens, R.J. Macchia, G.J. Wise, H.S. Wax, and D.S. Kim, Histochemical detection of steroid hormone receptors in the human prostate, in:”Prostate Cancer and Hormone Receptors,” G.P. Murphy and A.A. Sandberg, eds., Alan R. Liss, New York, (1980), p. 113.Google Scholar
  39. 39.
    G.C. Chamness, W.D. Mercer, and W.L. McGuire, Are histochemical methods for estrogen receptor valid?, J. Histochem. Cytochem. 28:792 (1980).PubMedCrossRefGoogle Scholar
  40. 40.
    J.B. Murphy, R.C. Emmott, L.L. Hicks, and P.C. Walsh, Estrogen receptors in the human prostate, seminal vesicle, epididymis, testis, and genital skin: a marker for estrogen-responsive tissues?, J.clin.Endocr. Metab. 50:938 (1980).PubMedCrossRefGoogle Scholar
  41. 41.
    R.A. Cowan, S.K. Cowan, and J.K. Grant, Binding of methyl-trienolone (R 1881) to a progesterone receptor-like component of human prostatic cytosol, J.Endocr. 74:281 (1977).PubMedCrossRefGoogle Scholar
  42. 42.
    E.J. Keenan, E.D. Kemp, E.E. Ramsey, L.B. Garrison, H.D. Pearse, and C.V. Hodges, Specific binding of prolactin by prostate gland of rat and man, J.Urol. 122:43 (1979).PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • M. A. Blankenstein
    • 1
  • J. C. Romijn
    • 1
  • G. J. van Steenbrugge
    • 1
  • F. H. Schröder
    • 1
  1. 1.Erasmus UniversityRotterdamThe Netherlands

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