Advertisement

DNA Probes: Development of Libraries

  • Henry F. Epstein
  • Stewart Wolf
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 154)

Abstract

DR. LOUIS M. KUNKEL: Dr. Latt has described some of the work currently in progress in our laboratory but has left to me the task of describing our work related to flow sorting and cloning the human X chromosome. The goal was to obtain a library of recombinant phage highly enriched for X chromosome DNA sequences. These phage would serve as sources of DNA possibly useful in linkage analysis with known X-linked loci or in the acquisition of the more than 100 known X-linked genes (52). I would like to describe briefly the construction of the X chromosome enriched library and our initial characterization of some of the human inserts from this library. Lastly, I would like to describe our initial efforts to obtain sequences of DNA which might possibly encode for X-linked proteins.

Keywords

Somatic Cell Hybrid Nick Translation Translocation Carrier Flow Histogram Recombinant Library 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Henry F. Epstein
    • 1
  • Stewart Wolf
    • 2
    • 3
  1. 1.The Jerry Lewis Neuromuscular Disease Research CenterBaylor College of MedicineHoustonUSA
  2. 2.Totts Gap Medical Research LaboratoriesBangorUSA
  3. 3.Temple UniversityPhiladelphiaUSA

Personalised recommendations