DNA Probes: Development of Libraries
DR. LOUIS M. KUNKEL: Dr. Latt has described some of the work currently in progress in our laboratory but has left to me the task of describing our work related to flow sorting and cloning the human X chromosome. The goal was to obtain a library of recombinant phage highly enriched for X chromosome DNA sequences. These phage would serve as sources of DNA possibly useful in linkage analysis with known X-linked loci or in the acquisition of the more than 100 known X-linked genes (52). I would like to describe briefly the construction of the X chromosome enriched library and our initial characterization of some of the human inserts from this library. Lastly, I would like to describe our initial efforts to obtain sequences of DNA which might possibly encode for X-linked proteins.
KeywordsSomatic Cell Hybrid Nick Translation Translocation Carrier Flow Histogram Recombinant Library
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