Initiation of Experimental Liver Carcinogenesis by Chemicals: Are the Carcinogen Altered Hepatocytes Stimulated to Grow into Foci by Different Selection Procedures Identical?

  • A. Columbano
  • G. M. Ledda
  • P. M. Rao
  • S. Rajalakshmi
  • D. S. R. Sarma
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 52)


The multi-stage nature of the carcinogenic process has been demonstrated in many tissues including skin (1–3), liver (4, 5), mammary gland (6), urinary bladder (7) and vagina (8). However, the molecular mechanisms underlying this complex process are not yet understood clearly. The development of several experimental model systems for the sequential analysis of chemical carcinogenesis in rat liver has opened up new avenues for investigating this complex process. Our experimental approaches have two main objectives (i) to understand the mechanisms underlying the initiation phase of liver carcinogenesis and (ii) to characterize the initiated hepatocytes stimulated to grow into enzyme altered foci by some of the selection procedures. The experimental design was guided by the hypothesis shown in Figure 1.


Selection Regimen Orotic Acid Chemical Carcinogenesis Positive Focus Liver Carcinogenesis 


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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • A. Columbano
    • 1
  • G. M. Ledda
    • 1
  • P. M. Rao
    • 1
  • S. Rajalakshmi
    • 1
  • D. S. R. Sarma
    • 1
  1. 1.Department of PathologyUniversity of TorontoTorontoCanada

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