Progress in the Study of inVitro Neoplastic Transformation
Since the pioneering work of Berwald and Sachs1,2 in 1963–1965, much effort has been made to develop adequate and useful mammalian cell systems for the study of the basic mechanisms of neoplastic transformation in chemical carcinogenesis. This constitutes a major aspect of this chapter. More recently, advances have been made to characterize neoplastic transformation at the molecular level; i.e. the study on neoplastic transformation has advanced from the animal model to the cell system, and then to the molecular description. While this approach is still in its infancy, some promising results have begun to appear. The description of this future trend will be included at the end of this chapter.
KeywordsSomatic Mutation Neoplastic Transformation Syrian Hamster Recessive Mutation Morphological Transformation
Unable to display preview. Download preview PDF.
- 1.Y. Berwald and L. Sachs,In vitro cell transformation with chemical carcinogens, Nature 200:1182–1184 (1963).Google Scholar
- 8.J.A. DiPaolo, R.L. Nelson, and P.J. Donovan, Morphological, oncogenic, and karyological characteristics of Syrian hamster embryo cells transformed in vitro by carcinogenic polycyclic hydrocarbons, Cancer Res. 31: 3573–3583 (1971).Google Scholar
- 11.L. Foulds, “Neoplastic Development, Vol. 1”, Academic Press, London (1969) and “Neoplastic Development, Vol. 2”, Academic Press, London (1975).Google Scholar
- 13.J.C. Barrett, B.D. Crawford, and P.O.P. Ts’o, The role of somatic mutation in a multistage model of carcinogenesis, in: “Advances in Modern Environmental Toxicology, Vol. 1, Mammalian Cell Transformation by Chemical Carcinogenesis”, N. Mishra, V. Dunkel and M. Mehlman, eds., Senate Press, Inc., Princeton, N.J. (1981).Google Scholar
- 14.Brian D. Crawford, Ph.D. Thesis, School of Hygiene and Public Health, The Johns Hopkins University (1981).Google Scholar
- 15.David Morry, Ph.D. Thesis, School of Hygiene and Public Health, The Johns Hopkins University (1980).Google Scholar
- 18.S.L. Lin, M. Takii and P.O.P. Ts’o, Somatic mutation and neoplastic transformation induced by [Methyl—3H]thymidine, Radiat. Res., in press (1982).Google Scholar
- 19.M. Zajac and P.O.P. Ts’o, In vitro neoplastic transformation induced by DNase I encapsulated in liposomes, Eur. J. Cell Biol. 22:1585, p. 533, Abstract of Second International Congress on Cell Biology (1980).Google Scholar
- 20.S.A. Bruce and P.O.P. Ts’o, Senescence and neoplastic transformation of Syrian hamster embryo and adult fibroblasts in vitro, Eur. J. Cell Biol. 22: 1642, p. 552, Abstract of Second International Congress on Cell Biology (1980).Google Scholar
- 21.S. Nakano and P.O.P. Ts’o, Cellular differentiation and neoplasia: Characterization of subpopulations of cells that have neoplasia—related growth properties in Syrian hamster embryo cell cultures, Proc. Nat. Acad. Sci. USA 78: 4995–4999 (1981).Google Scholar
- 22.D.L. Grady, R.K. Moyzis and P.O.P. Ts’o, An analysis of plasma membrane proteins from normal and highly tumorigenic Syrian hamster embryo cells by two dimensional gel electrophoresis, J. Cell Biol. 91, No.2, Part 2, Abst. 15061 (1981).Google Scholar
- 24.E.A. Arnold, W.S. Liaw and P.O.P. Ts’o, The use of cell cultures to assay the effects of chemicals on bone marrow, in:“Carcinogenesis: Fundamental Mechanisms and Environmental Effects,” B. Pullman, P.O.P. Ts’o and H. Gelboin, eds., D. Reidel Publishing Company, Dordrecht, Holland (1980).Google Scholar