Progress in the Study of inVitro Neoplastic Transformation

  • Paul O. P. Ts’o
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 52)


Since the pioneering work of Berwald and Sachs1,2 in 1963–1965, much effort has been made to develop adequate and useful mammalian cell systems for the study of the basic mechanisms of neoplastic transformation in chemical carcinogenesis. This constitutes a major aspect of this chapter. More recently, advances have been made to characterize neoplastic transformation at the molecular level; i.e. the study on neoplastic transformation has advanced from the animal model to the cell system, and then to the molecular description. While this approach is still in its infancy, some promising results have begun to appear. The description of this future trend will be included at the end of this chapter.


Somatic Mutation Neoplastic Transformation Syrian Hamster Recessive Mutation Morphological Transformation 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Y. Berwald and L. Sachs,In vitro cell transformation with chemical carcinogens, Nature 200:1182–1184 (1963).Google Scholar
  2. 2.
    Y. Berwald and L. Sachs, In vitro transformation of normal cells to tumor cells by carcinogenic hydrocarbons, J. Natl. Cancer Inst. 35:641–661 (1965).PubMedGoogle Scholar
  3. 3.
    J.C. Barrett, N.E. Bias and P.O.P. Ts’o, A mammalian cellular system for the concomitant study of neoplastic transformation and somatic mutation, Mutation Res. 50: 121–136 (1978).PubMedCrossRefGoogle Scholar
  4. 4.
    J.C. Barrett, B.D. Crawford, D.L. Grady, L.D. Hester, P.A. Jones, W.F. Benedict, and P.O.P. Ts’o, The temporal acquisition of enhanced fibrinolytic activity by Syrian hamster embryo cells following treatment with benzo(a)pyrene. Cancer Res. 37: 3815–3823 (1977).PubMedGoogle Scholar
  5. 5.
    J.C. Barrett and P.O.P. Ts’o, Evidence for the progressive nature of neoplastic transformation in vitro, Proc. Nat. Acad. Sci. USA 75: 3297–3301 (1978).PubMedCrossRefGoogle Scholar
  6. 6.
    J.A. DiPaolo and P.J. Donovan, Properties of Syrian hamster cells transformed in the •presence of carcinogenic hydrocarbons, Exptl. Cell Res. 48: 361–377 (1967).CrossRefGoogle Scholar
  7. 7.
    J.A. DiPaolo, P.J. Donovan and R. Nelson, Quantitative studies of in vitro transformation by chemical carcinogens, J. Nat. Cancer Inst. 42: 867–874 (1969).PubMedGoogle Scholar
  8. 8.
    J.A. DiPaolo, R.L. Nelson, and P.J. Donovan, Morphological, oncogenic, and karyological characteristics of Syrian hamster embryo cells transformed in vitro by carcinogenic polycyclic hydrocarbons, Cancer Res. 31: 3573–3583 (1971).Google Scholar
  9. 9.
    J.C. Barrett, B.D. Crawford, L.O. Mixter, L. M. Schectman, P.O.P. Ts’o and R. Pollack, Correlation of in vitro growth properties and tumorigenicity of Syrian hamster cell lines, Cancer Res. 39: 1504–1510 (1979).PubMedGoogle Scholar
  10. 10.
    L. Foulds, The experimental study of tumor progression: A review, Cancer Res. 14: 327–339 (1954).PubMedGoogle Scholar
  11. 11.
    L. Foulds, “Neoplastic Development, Vol. 1”, Academic Press, London (1969) and “Neoplastic Development, Vol. 2”, Academic Press, London (1975).Google Scholar
  12. 12.
    J.C. Barrett and P.O.P. Ts’o, The relationship between somatic mutation and neoplastic transformation, Proc. Nat. Acad. Sci. USA 75: 3297–3301 (1978).PubMedCrossRefGoogle Scholar
  13. 13.
    J.C. Barrett, B.D. Crawford, and P.O.P. Ts’o, The role of somatic mutation in a multistage model of carcinogenesis, in: “Advances in Modern Environmental Toxicology, Vol. 1, Mammalian Cell Transformation by Chemical Carcinogenesis”, N. Mishra, V. Dunkel and M. Mehlman, eds., Senate Press, Inc., Princeton, N.J. (1981).Google Scholar
  14. 14.
    Brian D. Crawford, Ph.D. Thesis, School of Hygiene and Public Health, The Johns Hopkins University (1981).Google Scholar
  15. 15.
    David Morry, Ph.D. Thesis, School of Hygiene and Public Health, The Johns Hopkins University (1980).Google Scholar
  16. 16.
    J.C. Barrett, T. Tsutsui, and P.O.P. Ts’o, Neoplastic transformation induced by a direct perturbation of DNA, Nature 274: 229–232 (1978).PubMedCrossRefGoogle Scholar
  17. 17.
    T. Tsutsui, J.C. Barrett and P.O.P: Ts’o, Chromosomal aberrations, DNA damage and morphological transformation of synchronized Syrian hamster embryo cells: Effect of 5– bromodeoxyuridine and near ultraviolet radiation, Cancer Res. 39: 2356–2365 (1979).PubMedGoogle Scholar
  18. 18.
    S.L. Lin, M. Takii and P.O.P. Ts’o, Somatic mutation and neoplastic transformation induced by [Methyl—3H]thymidine, Radiat. Res., in press (1982).Google Scholar
  19. 19.
    M. Zajac and P.O.P. Ts’o, In vitro neoplastic transformation induced by DNase I encapsulated in liposomes, Eur. J. Cell Biol. 22:1585, p. 533, Abstract of Second International Congress on Cell Biology (1980).Google Scholar
  20. 20.
    S.A. Bruce and P.O.P. Ts’o, Senescence and neoplastic transformation of Syrian hamster embryo and adult fibroblasts in vitro, Eur. J. Cell Biol. 22: 1642, p. 552, Abstract of Second International Congress on Cell Biology (1980).Google Scholar
  21. 21.
    S. Nakano and P.O.P. Ts’o, Cellular differentiation and neoplasia: Characterization of subpopulations of cells that have neoplasia—related growth properties in Syrian hamster embryo cell cultures, Proc. Nat. Acad. Sci. USA 78: 4995–4999 (1981).Google Scholar
  22. 22.
    D.L. Grady, R.K. Moyzis and P.O.P. Ts’o, An analysis of plasma membrane proteins from normal and highly tumorigenic Syrian hamster embryo cells by two dimensional gel electrophoresis, J. Cell Biol. 91, No.2, Part 2, Abst. 15061 (1981).Google Scholar
  23. 23.
    R.K. Moyzis, D.L. Grady, D.W. Li, S.E. Mirvis and P.O.P. Ts’o, Extensive homology of nuclear ribonucleic acid and polysomal poly(adenylic acid) messenger ribonucleic acid between normal and neoplastically transformed cells, Biochemistry 19: 821–832 (1980).PubMedCrossRefGoogle Scholar
  24. 24.
    E.A. Arnold, W.S. Liaw and P.O.P. Ts’o, The use of cell cultures to assay the effects of chemicals on bone marrow, in:“Carcinogenesis: Fundamental Mechanisms and Environmental Effects,” B. Pullman, P.O.P. Ts’o and H. Gelboin, eds., D. Reidel Publishing Company, Dordrecht, Holland (1980).Google Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Paul O. P. Ts’o
    • 1
  1. 1.Division of Biophysics, School of Hygiene and Public HealthThe Johns Hopkins UniversityBaltimoreUSA

Personalised recommendations