Immunological Studies with Tobacco Mosaic Virus Protein: Differential Activation of B Cell Subpopulations

  • Donna M. Rennick
  • Philip R. Morrow
  • Eli Benjamini
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 150)


Currently, information concerning the activation requirements of B lymphocytes in different stages of differentiation is limited. It has been known for some time that resting B lymphocytes at different levels of maturation may not respond to the same stimuli in the same way. Activation of immunocompetent lymphocytes from adult animals by T-dependent (TD) antigens can lead to antibody production and memory whereas stimulation of immature lymphocytes (neonatal) may under identical conditions result in tolerance.1–4 Furthermore, it appears that primary and memory B cells do not exhibit the same requirements for activation under certain experimental conditions. Although some of these results may be explained by quantitative differences, there is evidence for qualitative differences as well.5–9


Antibody Response Memory Regeneration Brucella Abortus Immunoglobulin Isotype Composite Binding 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    E. S. Metcalf and N. R. Klinman, In vitro tolerance induction of neonatal murine B cells, J. Exp. Med. 143:1327 (1977).Google Scholar
  2. 2.
    J. C. Cambier, E. S. Vitetta, J. W. Uhr and J. R. Kettman, B cell tolerance. II. Trinitrophenyl human gammaglobulininduced tolerance in adult and neonatal murine B cells responsive to thymus-dependent and independent forms of the same hapten, J. Exp.-Med. 145: 778 (1977).PubMedCrossRefGoogle Scholar
  3. 3.
    H. M. Etlinger and J. M. Chiir, Maturation of the lymphoid system. II. Characterization of the cellular levels of unresponsiveness induced in neonates by a T-dependent antigen that is an obligate immunogen in adults, J. Immunol. 122: 2564 (1979).PubMedGoogle Scholar
  4. 4.
    G. J. V. Nossal and B. L. Pike, Evidence for the clonal abortion theory of B lymphocyte tolerance, J. Exp. Med. 141: 904 (1975).PubMedGoogle Scholar
  5. 5.
    N. R. Klinman, J. L. Press and G. P. Segal, Overlap stimulation of primary and secondary B cells by cross-reactive determinants, J. Exp. Med. 138: 1276 (1973).PubMedCrossRefGoogle Scholar
  6. 6.
    D. H. Katz, The allogeneic effect, in: “Immunopotentiation,” Ciba Foundation Symposium No. 18 G. E. W. Wolstenholme and J. Knight, eds., Associated Scientific Publishers, Amsterdam (1973).Google Scholar
  7. 7.
    S. Cammisuli and C. Henry, Role of membrane receptors in the induction of an in vitro secondary anti-hapten response. II. Antigen-immunoglobulin receptor interaction is not required for B memory cell proliferation, Eur. J. Immunol. 8: 622 (1978).Google Scholar
  8. 8.
    S. Cammisuli, C. Henry and L. Wofsy, Role of membrane receptors in the induction of an in vitro secondary anti-hapten response. I. Differentiation of B memory cells to plasma cells is independent of antigen-immunoglobulin receptor interaction, Eur. J. Immunol. 8:656 (1978).Google Scholar
  9. 9.
    D. M. Rennick, P. R. Morrow, C. Y. Leung, R. J. Scibienski and E. Benjamini, Differential activation of primary and memory •B. lymphocytes, J. Immunol. 125: 1262 (1980).PubMedGoogle Scholar
  10. 10.
    S. Strober, Immune function cell surface characteristics and maturation of B cell subpopulations, Transplant. Rev. 24: 84 (1975).PubMedGoogle Scholar
  11. 11.
    M. V. Hobbs and T. L. Feldbush, Antigen modulation of the immune response. VI. Rate of large memory cell appearance in lymph nodes and thoracic duct lymph, Cell. Immunol. 50: 30 (1980).Google Scholar
  12. 12.
    H. G. Durkin and G. J. Thorbecke, Homing of immunologically committed lymph node cells to germinal centers in rabbits, Nature-New Biol. 238: 53 (1972).PubMedGoogle Scholar
  13. 13.
    S. P. Lerman, T. J. Romano, J. J. Mond, M. Heidelberger and G. J. Thorbecke, Induction of primary and inhibition of secondary antibody response to hapten by hapten conjugates of type III pneumococcal polysaccharide, Cell. Immunol. 15: 321 (1975).Google Scholar
  14. 14.
    W. E. Paul, B. Benacerraf, B. W. Siskind, E. A. Goidl and R. A. Reisfeld, The anamnestic antibody response to type III specific pneumococcal polysaccharide, J. Exp. Med. 130: 77 (1969).PubMedCrossRefGoogle Scholar
  15. 15.
    H. Braley-Mullen, Secondary IgG responses to type 3 pneumococcal polysaccharide. III. T cell requirement for development of B memory cells, Eur. J. Immunol. 7: 775 (1977).PubMedCrossRefGoogle Scholar
  16. 16.
    T. V. Tittle and M. B. Rittenberg, Expression of IgG memory response in vitro to thymus-dependent and thymus-independent antigens, Cell. Immunol. 35: 180 (1978).Google Scholar
  17. 17.
    D. T. Umetsu, J. M. Chapman-Alexander and G. J. Thorbecke, Cross-priming of murine B cells with TNP conjugates of hemocyanin and ficoll: characteristics of primed B cells responding to both antigens. J. Immunol. 123: 396 (1979).PubMedGoogle Scholar
  18. 18.
    T. V. Tittle and M. B. Rittenberg, Distinct subpopulations of IgG memory B cells respond to different molecular forms of the same hapten, J. Immunol. 121: 936 (1978).PubMedGoogle Scholar
  19. 19.
    E. Benjamini, Immunochemistry of the tobacco mosaic virus protein, in: “Immunochemistry of Proteins,” M. Z. Atassi, ed., Plenum Press, New York (1977).Google Scholar
  20. 20.
    R. B. Merrifield, Solid phase peptide synthesis. III. An improved synthesis of bradykinin, Biochem. 3: 1385 (1964).CrossRefGoogle Scholar
  21. 21.
    F. J. Fearney, C. Y. Leung, J. D. Young, and E. Benjamini, The specificity of antibodies to a peptide determinant of the tobacco mosaic virus protein induced by immunization with the peptide conjugate, Biochem. Biophys. Acta 243: 509 (1971).Google Scholar
  22. 22.
    T. T. Tsu and L. A. Herzenberg, Solid-phase radioimmune assays,in: “Selected Methods in Cellular Immunology,” B. B. Mishell and S. M. Shiigi, eds., W. H. Freeman and Co., San Francisco (1980).Google Scholar
  23. 23.
    G. K. Lewis and J. W. Goodman, Carrier-directed anti-hapten responses by B-cell subsets, J. Exp. Med. 146: 1 (1977).PubMedCrossRefGoogle Scholar
  24. 24.
    H. S. Boswell, A Ahmed, I. Scher and A. Singer, Role of assessory cells in B cell activation. II. The interaction of B cells with accessory cells results in the exclusive activation of an Lyb5+ B cell population, J. Immunol. 125: 1340 (1980).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Donna M. Rennick
    • 1
  • Philip R. Morrow
    • 1
  • Eli Benjamini
    • 1
  1. 1.Department of Medical Microbiology, School of MedicineUniversity of CaliforniaDavisUSA

Personalised recommendations