Investigations on Renal Prostaglandins by Gas Chromatography—Mass Spectrometry

  • Ingrid W. Reimann
  • Christine Fischer
  • Bernd Rosenkranz
  • Jürgen C. Frölich


The large number of arachidonic acid metabolites present in the mammalian kidney and urine require highly sensitive and specific analytical methods for the determination of very small amounts of chemically closely related prostanoids. Gas chromatography—mass spectrometry (GCMS) combines a high degree of specificity and sensitivity, especially if preceded by high-performance liquid chromatographic (HPLC) purification and preseparation of biological samples. A further improvement of sensitivity is added by using glass capillary columns instead of the “classical,” standard packed columns. Elucidation of the role of arachidonic acid metabolites in renal physiology and pathophysiology necessitates both a highly sophisticated analytical method as well as special marker prostaglandins (PGs) to serve as indicators for the different renal functions.


Nephrogenic Diabetes Insipidus Renin Release Arachidonic Acid Metabolite Central Diabetes Insipidus Renal Prostaglandin 
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Copyright information

© Plenum Publishing Corporation 1983

Authors and Affiliations

  • Ingrid W. Reimann
    • 1
  • Christine Fischer
    • 1
  • Bernd Rosenkranz
    • 1
  • Jürgen C. Frölich
    • 1
  1. 1.Dr. Margarete Fischer-Bosch-Institut für Klinische PharmakologieStuttgartFederal Republic of Germany

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