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Investigations of Renal Arachidonic Acid Metabolites by Radioimmunoassay

  • Francesco Pugliese
  • Giovanni Ciabattoni

Abstract

This chapter describes (1) the application of radioimmunoassay (RIA) techniques to the investigation of renal arachidonic acid metabolism in humans; (2) methodological problems encountered in the detection of unmetabolized prostaglandins (PGs) in human urine; (3) the relevance of these techniques to the study of physiology and pathophysiology of the renal PG system; and (4) evidence in favor of a renal origin of urinary prostacyclin (PGI2) breakdown product, i.e., 6-keto-PGF, and thromboxane (TX) B2 in humans. It is not our purpose to review all the modifications of RIA techniques that have been used over the years by different authors, but to present in detail the problems which are relevant to a correct RIA measurement of arachidonic acid metabolites in complex biological fluids.

Keywords

Plasma Renin Activity Mean Blood Pressure Arachidonic Acid Metabolite Renal Origin Urinary Prostaglandin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Frölich JC (ed) Methods in Prostaglandin Research, Advances in Prostaglandin Thromboxane Research, vol 5, 1978.Google Scholar
  2. 2.
    Moncada S, Gryglewski RJ, Bunting S, et al: An enzyme isolated from arteries transforms prostaglandin endoperoxides to an unstable substance that inhibits platelet aggregation. Nature 263: 663, 1976.PubMedCrossRefGoogle Scholar
  3. 3.
    Granström E: Radioimmunoassay of prostaglandins. Prostaglandins 15: 3–17, 1978.PubMedCrossRefGoogle Scholar
  4. 4.
    Handler JS, Perkins FM, Johnson JP: Studies of renal cell function using cell culture techniques. Am J Physiol 238: F1 - F9, 1980.PubMedGoogle Scholar
  5. 5.
    Foidart JB, Dechenne C, Mahieu P, et al: Tissue culture of normal glomeruli: Isolation and morphological characterization of two homogeneous cell lines. Invest Cell Pathol 2: 15, 1979.PubMedGoogle Scholar
  6. 6.
    Mc Giff JC, Wong PYK: Compartmentalization of prostaglandins and prostacyclin within the kidney: Implications for renal function. Fed Proc 38: 89, 1979.Google Scholar
  7. 7.
    Dunn MJ, Hood VL: Prostaglandins and the kidney. Am J Physiol 233: F169 - F184, 1977.Google Scholar
  8. 8.
    Patrono C, Wennmalm A, Ciabattoni G, et al: Evidence for an extra-renal origin of urinary prostaglandin E2 in healthy men. Prostaglandins 18: 623–629, 1979.PubMedCrossRefGoogle Scholar
  9. 9.
    Patrono C, Ciabattoni G, Pinca E, et al: Low dose aspirin and inhibition of Thromboxane B2 production in healthy subjects. Thromb Res 17: 317–327, 1980.PubMedCrossRefGoogle Scholar
  10. 10.
    Dunn MJ, Liard JF, Dray F: Basal and stimulated rates of renal secretion and excretion of prostaglandins E2, F and 13–14 dihydro-15-keto-F2a. Kidney Int 13: 136–143, 1978.CrossRefGoogle Scholar
  11. 11.
    Frölich JC, Wilson TW, Sweetman BJ, et al: Urinary prostaglandins: Identification and origin. J Clin Invest 55: 763–770, 1975.PubMedCrossRefGoogle Scholar
  12. 12.
    Änggârd E, Samuelsson B: Prostaglandins and related factors. Metabolism of prostaglandin E1 in guinea pig lung. The structures of two metabolites. J Biol Chem 239: 4097–4102, 1964.Google Scholar
  13. 13.
    Änggârd E, Larsson C, Samuelsson B: The distribution of 15-hydroxy-prostaglandin dehydrogenase and prostaglandin 6,13-reductase in tissue of the swine. Acta Physiol Scand 81: 396–404, 1971.PubMedCrossRefGoogle Scholar
  14. 14.
    Raz A: Interaction of prostaglandins with blood plasma proteins. Biochem J 130: 631–636, 1972.PubMedGoogle Scholar
  15. 15.
    Gill JR, Frölich JC, Bowden RE, et al: Bartter’s syndrome: A disorder characterized by high urinary prostaglandins and a dependence of hyperreninemia on prostaglandin synthesis. Am J Med 61: 43–45, 1976.PubMedCrossRefGoogle Scholar
  16. 16.
    Abe K, Yasujima M, Chiba S, et al: Effect of furosemide on urinary excretion of prostaglandin E in normal volunteers and patients with essential hypertension. Prostaglandins 14: 513–521, 1977.Google Scholar
  17. 17.
    Zia P, Speckart P, Zipser R, et al: A radioimmunoassay of prostaglandin E for human plasma and urine. Prostaglandins 13: 1005, 1977.Google Scholar
  18. 18.
    Dray F: Bartter’s syndrome: Contrasting patterns of prostaglandin excretion in children and adults. Clin Sci Mol Med 54: 115–118, 1978.PubMedGoogle Scholar
  19. 19.
    Scherer B, Schnermann J, Sofroniew M, et al: Prostaglandin (PG) analysis in urine of humans and rats by different radioimmunoassays: Effect on PG excretion by PG synthetase inhibitors, laparotomy and furosemide. Prostaglandins 15: 255–256, 1978.PubMedCrossRefGoogle Scholar
  20. 20.
    Tan SY, Sweet P, Mulrow PJ: Impaired renal production of prostaglandin E2: A newly identified lesion in human essential hypertension. Prostaglandins 15: 139–149, 1978.PubMedCrossRefGoogle Scholar
  21. 21.
    Ciabattoni G, Pugliese F, Spaldi M, et al: Radioimmunoassay measurement of prostaglandin E2 and Fla in human urine. J Endocrinol Invest 2: 173–182, 1979.PubMedGoogle Scholar
  22. 22.
    Brater DC, Beck JM, Adams BV, et al: Effects of indomethacin on furosemide-stimulated urinary PGE2 excretion in man. Eur J Pharmacol 65: 213–219, 1980.PubMedCrossRefGoogle Scholar
  23. 23.
    Seyberth HW, Müller H, Erlenmeier T, et al: Mass spectrometric determination of urinary prostaglandins in preterm infants. Eur J Clin Pharmacol 18: 89–93, 1980.PubMedCrossRefGoogle Scholar
  24. 24.
    Dusing R, Bartter FC, Gill JR, et al: Effects of moderate short-term potassium depletion in normal humans. The role of prostaglandins. Prostaglandins 20: 971–979, 1980.PubMedCrossRefGoogle Scholar
  25. 25.
    Haluska PV, Margolius HS, Allen H: Urinary Excretion of prostaglandin E like material and kallikrein: Effects of furosemide. Prostaglandins 18: 359–368, 1979.Google Scholar
  26. 26.
    Ciabattoni G, Pugliese F, Pinca E, et al: Biologic and methodologic variables affecting urinary prostaglandin measurement. Adv Prost Thromb Res 6: 207–212, 1980.Google Scholar
  27. 27.
    Ciabattoni G, Pugliese F, Cinotti GA, et al: Methodologie problems in the radioimmunoassay of prostaglandin E2 and F2a, in human urine, in Albertini A, Da Prada M, Peskar BA (eds): Radioimmunoassay of Drugs and Hormones in Cardiovascular Medicine. Amsterdam, New York, Oxford, Elsevier/North-Holland, 1979, p 265.Google Scholar
  28. 28.
    Patrono C, Ciabattoni G, Cinotti GA, et al: Characterization of human urinary prostaglandin-like immunoreactivity. Prostaglandins 15: 700–701, 1978.CrossRefGoogle Scholar
  29. 29.
    Templeton AA, Cooper I, Kelly RW: J Reprod Fertil 52: 147–150, 1978.PubMedCrossRefGoogle Scholar
  30. 30.
    Sun FF, Taylor BM, Sutter DM, et al: Metabolism of prostacyclin. III. Urinary metabolite profile of 6-keto-PGFic, in rat. Prostaglandins 17: 753–759, 1979.PubMedCrossRefGoogle Scholar
  31. 31.
    Bianchi-Porro G, Caruso I, Ciabattoni G, et al: Comparative effects of aspirin and diflunisal on platelet and gastric prostaglandin synthetase in humans. Br J Pharmacol 72: 141–142, 1981.Google Scholar
  32. 32.
    Di Munno O, Bombardieri S, Pasero G, et al: The synovial prostaglandin system in chronic inflammatory arthritis. Differential effects of steroidal and non steroidal antiinfiammatory drugs. Br J Pharmacol, 73: 893–901, 1981.PubMedGoogle Scholar
  33. 33.
    Oliw E, Lunden I, Sjöquist B, et al: Determination of 6-keto-prostaglandin Fla, in rabbit kidney and urine and its relation to sodium balance. Acta Physiol Scand 105: 359, 1979.PubMedCrossRefGoogle Scholar
  34. 34.
    Patrono C, Ciabattoni G, Cinotti GA, et al: Prostacyclin and renin release in man. Clin Res 27: 426A, 1979.Google Scholar
  35. 35.
    Patrono C, Cinotti GA, Ciabattoni G, et al: Prostaglandins, thromboxane and renal physiology: Introduction, in Remuzzi G, Mecca G, De Gaetano G (eds): Hemostasis, Prostaglandins and Renal Disease. New York, Raven Press, 1980, p 151.Google Scholar
  36. 36.
    Grenier F, Smith W: Formation of 6-keto-PGF1a, by collecting tube cells isolated from rabbit renal papillae. Prostaglandins 16: 759–771, 1978.CrossRefGoogle Scholar
  37. 37.
    Hassid A, Dunn M: Microsomal prostaglandin biosynthesis of human kidney. J Biol Chem 255: 2472–2475, 1979.Google Scholar
  38. 38.
    Ciabattoni G, Pugliese F, Cinotti GA, et al: Characterization of furosemide induced activation of the renal prostaglandin system. Eur J Pharmacol 60: 181–187, 1979.PubMedCrossRefGoogle Scholar
  39. 38.
    Ciabattoni G, Pugliese F, Cinotti GA, et al: Characterization of furosemide induced activation of the renal prostaglandin system. Eur J Pharmacol 60: 181–187, 1979.PubMedCrossRefGoogle Scholar
  40. 40.
    O’Grady J, Warrington S, Moti MJ, et al: Effects of intravenous infusion of prostacyclin (PGI2) in man. Prostaglandins 19: 319–332, 1980.PubMedCrossRefGoogle Scholar
  41. 41.
    Rosenkranz B, Fischer C, Weimer KE, et al: Metabolism of prostacyclin and 6-keto-PGF1, in man. J Biol Chem 255: 10194–10198, 1980.PubMedGoogle Scholar
  42. 42.
    Dusting GJ, Moncada S, Vane JR: Br J Pharmacol 64: 315–320, 1978.PubMedGoogle Scholar
  43. 43.
    Demers LM, Derck DD: A radioimmunoassay for 6-keto-PGF1a. Adv Prost Thromb Res 6: 193–198, 1980.Google Scholar
  44. 44.
    Hensby CN, Fitzgerald GA, Friedman LA, et al: Measurement of 6-oxo-PGF1 in human plasma using gas chromatography—mass spectrometry. Prostaglandins 18: 731–736, 1979.PubMedCrossRefGoogle Scholar
  45. 45.
    Fitzgerald GA, et al: poster presentation at the Winter Prostaglandin Conference, Clearwater, Florida, March 1–5, 1981.Google Scholar
  46. 46.
    Ciabattoni G, Pugliese F, Cinotti GA, et al: Renal effects of anti-inflammatory drugs. Eur J Rheumatol 3: 210–221, 1980.Google Scholar
  47. 47.
    Morrison AR, Nishikawa K, Needleman P: Thromboxane A2 biosynthesis in the ureter obstructed isolated perfused kidney of the rabbit. J Pharmacol Exp Ther 205: 1–8, 1978.PubMedGoogle Scholar
  48. 48.
    Larsson C: The renal prostaglandin system; localization and some biological effects. Contrib Nephrol 12: 82, 1978.PubMedGoogle Scholar
  49. 49.
    Hassid A, Konieczkowski M, Dunn MJ: Prostaglandin synthesis in isolated rat kidney glomeruli. Proc Nat Acad Sci USA 76: 1155–1159, 1979.PubMedCrossRefGoogle Scholar
  50. 50.
    Burch JW, Stanford N, Majerus PW: Inhibition of platelet prostaglandin synthetase by oral aspirin. J Clin Invest 61: 314–319, 1978.PubMedCrossRefGoogle Scholar
  51. 51.
    Patrignani P, Cattani P, Minuz P, et al: Low dose aspirin: How low, how often? Clin Res 29: 343A, 1981.Google Scholar

Copyright information

© Plenum Publishing Corporation 1983

Authors and Affiliations

  • Francesco Pugliese
    • 1
  • Giovanni Ciabattoni
    • 2
  1. 1.Il Clinica MedicaUniversity of RomeRomeItaly
  2. 2.Department of PharmacologyCatholic University School of MedicineRomeItaly

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