Investigations of Renal Arachidonic Acid Metabolites by Radioimmunoassay

  • Francesco Pugliese
  • Giovanni Ciabattoni


This chapter describes (1) the application of radioimmunoassay (RIA) techniques to the investigation of renal arachidonic acid metabolism in humans; (2) methodological problems encountered in the detection of unmetabolized prostaglandins (PGs) in human urine; (3) the relevance of these techniques to the study of physiology and pathophysiology of the renal PG system; and (4) evidence in favor of a renal origin of urinary prostacyclin (PGI2) breakdown product, i.e., 6-keto-PGF, and thromboxane (TX) B2 in humans. It is not our purpose to review all the modifications of RIA techniques that have been used over the years by different authors, but to present in detail the problems which are relevant to a correct RIA measurement of arachidonic acid metabolites in complex biological fluids.


Plasma Renin Activity Mean Blood Pressure Arachidonic Acid Metabolite Renal Origin Urinary Prostaglandin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Plenum Publishing Corporation 1983

Authors and Affiliations

  • Francesco Pugliese
    • 1
  • Giovanni Ciabattoni
    • 2
  1. 1.Il Clinica MedicaUniversity of RomeRomeItaly
  2. 2.Department of PharmacologyCatholic University School of MedicineRomeItaly

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