Perfusion-Dependent Selective Induction of Prostaglandin Biosynthesis Activity in Rabbit Kidney Cortex
Renal prostaglandin release, induced by peptide hormones, involves a selective deacylation of esterified arachidonic acid in a tightly coupled process in which a major portion (25–50%) of the released arachidonate is converted to prostaglandins.1,2 In the ureter-obstructed kidney model employed in many of these studies, there is a time-dependent increase in prostaglandin release in response to bradykinin or angiotensin II stimulation. This enhanced prostaglandin release is the result of a de novo gradual synthesis of both a hormone-sensitive lipase and prostaglandin synthase enzymes during the perfusion.2,3 A major question yet to be resolved concerns the renal cellular site in which the newly synthesizing prostaglandin synthase activity is located. This report provides evidence that the newly synthesized prostaglandin synthase is found in the cortex of the perfused, ureter-obstructed kidney.
KeywordsArachidonic Acid Cortical Slice Rabbit Kidney Prostaglandin Release PGE2 Generation
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