Biosynthesis of Prostaglandins and Thromboxanes in the Dog Kidney

  • Takeshi Okahara
  • Masahito Imanishi
  • Kenjiro Yamamoto


It has been postulated that renal tissue may convert arachidonic acid (AA) to several prostaglandins (PGs) as well as thromboxane (TX) A2, because this tissue contains a comparatively large amount of AA1 and also significant amounts of the cyclooxygenase enzyme in the various areas of the kidney.2 Actually, PGE2 and PGF, known as renal PG, are mainly synthesized in the medulla of all the laboratory animals examined and in humans.3,4 The biosynthesis of PGI2 has been demonstrated in the cortex of rats and rabbits.5,6 TXA2, another AA metabolite, was detected only in kidneys with an obvious pathological status.7,8 Although there is considerable agreement with regard to the compartmentalization of PGI2 and the virtual absence of TXA2 biosynthesis within the kidney, recent experiments revealed that PGI2 was also a renomedullary PG, as are PGE2 and PGF,4,9–12 and that human renal microsomes synthesized significant amounts of TXA2, measured as TXB2.4


Arachidonic Acid Secretion Rate Ureteral Obstruction Arachidonic Acid Metabolite Rabbit Kidney 
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  1. 1.
    Comai K, Farber SJ, Paulsrud JR: Analysis of renal medullary lipid droplets from normal, hydronephrotic and indomethacin-treated rabbits. Lipids 10: 555–561, 1975.PubMedCrossRefGoogle Scholar
  2. 2.
    Smith WL, Bell TG: Immunohistochemical localization of the prostaglandin-forming cyclooxygenase in renal cortex. Am J Physiol 235: F451 - F457, 1978.PubMedGoogle Scholar
  3. 3.
    Dunn MJ, Hood VL: Prostaglandins and the kidney. Am J Physiol 233: F169 - F184, 1977.Google Scholar
  4. 4.
    Hassid A, Dunn MJ: Microsomal prostaglandin biosynthesis of human kidney. J Biol Chem 255: 2472–2475, 1980.PubMedGoogle Scholar
  5. 5.
    Whorton AR, Smigel M, Oates JA, et al: Regional differences in prostacyclin formation by the kidney. Biochim Biophys Acta 529: 176–180, 1978.PubMedGoogle Scholar
  6. 6.
    Zenser TV, Herman CA, Gorman RR, et al: Metabolism and action of the prostaglandin endoperoxide PGH2 in rat kidney. Biochem Biophys Res Commun 79: 357–363, 1977.PubMedCrossRefGoogle Scholar
  7. 7.
    Morrison AR, Nishikawa K, Needleman P: Unmasking of thromboxane A2 synthesis by ureteral obstruction in the rabbit kidney. Nature 267: 259–260, 1977.PubMedCrossRefGoogle Scholar
  8. 8.
    Morrison AR, Nishikawa K, Needleman P: Thromboxane A2 biosynthesis in the ureter-obstructed isolated perfused kidney of the rabbit. J Pharmacol Exp Ther 205: 1–8, 1978.PubMedGoogle Scholar
  9. 9.
    Silberbauer K, Sinzinger H: Cortex and medulla of rat kidney generate different amounts of PGI2-like activity. Thromb Res 13: 1111–1118, 1978.PubMedCrossRefGoogle Scholar
  10. 10.
    Oliw E, Lunden I, Sjöquist B, et al: Determination of 6-keto-prostaglandin Fla in rabbit kidney and urine and its relation to sodium balance. Acta Physiol Scand 105: 359–366, 1979.CrossRefGoogle Scholar
  11. 11.
    Satoh H, Satoh S: Prostaglandin formation by microsomes of dog kidney. Biochem Biophys Res Commun 14: 1266–1272, 1980.CrossRefGoogle Scholar
  12. 12.
    Grenier FC, Smith WL: Formation of 6-keto-PGF1. by collecting tubule cells isolated from rabbit renal papillae. Prostaglandins 16: 759–772, 1978.CrossRefGoogle Scholar
  13. 13.
    Okahara T, Manchandia M, Michelakis AM, et al: The renin—angiotensin system and renal prostaglandin E2 release in dogs. Proc Soc Exp Biol Med 166: 57–63, 1981.PubMedGoogle Scholar
  14. 14.
    Moncada S, Vane JR: Pharmacology and endogenous roles of prostaglandin endoperoxides, thromboxane A2, and prostacyclin. Pharmacol Rev 30: 293–331, 1979.Google Scholar
  15. 15.
    Barger AC, Herd JA: Renal vascular anatomy and distribution of blood flow, in Orloff J, Berliner RW (eds): Renal Physiology. Washington, DC, American Physiological Society, 1973, p 249.Google Scholar
  16. 16.
    Dunn MJ, Liard JF, Dray F: Basal and stimulated rates of renal secretion and excretion of prostaglandin E2, F. and 13,14-dihydro-15-keto-Fa in the dog. Kidney Int 13: 136–143, 1978.CrossRefGoogle Scholar
  17. 17.
    Wong PY-K, McGiff JC, Cagen L, et al: Metabolism of prostacyclin in rabbit kidney. J Biol Chem 254: 12–14, 1979.PubMedGoogle Scholar
  18. 18.
    Okahara T, Abe Y, Imanishi M, et al: Effect of calcium on prostaglandin E2 release in dogs. Am J Physiol 241: F77 - F84, 1981.PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1983

Authors and Affiliations

  • Takeshi Okahara
    • 1
  • Masahito Imanishi
    • 1
  • Kenjiro Yamamoto
    • 1
  1. 1.Department of PharmacologyOsaka City University Medical SchoolOsakaJapan

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