Regional Differences in in Vitro Prostaglandin Synthesis by the Rat Kidney

  • Josée Sraer
  • Wolfgang Siess
  • Fernand Dray
  • Raymond Ardaillou


Numerous studies over the last five years have been devoted to the study of in vitro prostaglandin (PG) production by different parts of the kidney from various species. Both fresh preparations1–5 and cultured cells6,7 have been studied. The techniques used in these reports were radioimmunoassay (RIA) without preliminary purification and radiometric thin-layer chromatography. Moreover, there has been no systematic study of PG production in every part of the kidney from a given species. Thus we decided to try to fill this gap by measuring PG production in a variety of structures from the rat kidney. We used, for this purpose, a precise quantitative technique—RIA after high-performance liquid chromatography (HPLC) purification.


Mesangial Cell Prostaglandin Synthesis Outer Medulla Glomerular Mesangial Cell Unidentified Peak 
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Copyright information

© Plenum Publishing Corporation 1983

Authors and Affiliations

  • Josée Sraer
    • 1
  • Wolfgang Siess
    • 2
  • Fernand Dray
    • 2
  • Raymond Ardaillou
    • 1
  1. 1.INSERM U64Hôpital TenonParisFrance
  2. 2.INSERM U207Institut PasteurParisFrance

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