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Regional Differences in in Vitro Prostaglandin Synthesis by the Rat Kidney

  • Josée Sraer
  • Wolfgang Siess
  • Fernand Dray
  • Raymond Ardaillou

Abstract

Numerous studies over the last five years have been devoted to the study of in vitro prostaglandin (PG) production by different parts of the kidney from various species. Both fresh preparations1–5 and cultured cells6,7 have been studied. The techniques used in these reports were radioimmunoassay (RIA) without preliminary purification and radiometric thin-layer chromatography. Moreover, there has been no systematic study of PG production in every part of the kidney from a given species. Thus we decided to try to fill this gap by measuring PG production in a variety of structures from the rat kidney. We used, for this purpose, a precise quantitative technique—RIA after high-performance liquid chromatography (HPLC) purification.

Keywords

Mesangial Cell Prostaglandin Synthesis Outer Medulla Glomerular Mesangial Cell Unidentified Peak 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Publishing Corporation 1983

Authors and Affiliations

  • Josée Sraer
    • 1
  • Wolfgang Siess
    • 2
  • Fernand Dray
    • 2
  • Raymond Ardaillou
    • 1
  1. 1.INSERM U64Hôpital TenonParisFrance
  2. 2.INSERM U207Institut PasteurParisFrance

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