Cellular Compartmentalization of the Biosynthesis and Function of PGE2 and PGI2 in the Renal Medulla
It is clear from studies conducted in a number of laboratories that the biosynthesis of prostaglandins in the kidney is compartmentalized (Fig. 1). Compartmentalization of synthesis is apparently associated with compartmentalization of function. For example, in the renal tubule there is evidence that prostaglandins are formed by the thin limb of Henle’s loop, at least in the hydronephrotic rabbit kidney,1 by the thick ascending limb,2 and by the cortical and medullary collecting tubules,3–6 and that prostaglandins affect ion or water transport7–9 and/or adenylate cyclase activities10,11 in each of these regions of the tubule. Thus one of the major goals of studies on renal prostaglandin biochemistry has been that of localization—to define which cells form prostaglandins, to determine which of the active prostaglandin derivatives are formed by each of these cells, and, finally, to determine the site and mechanism of action of prostaglandins formed at the different locales. The rationale for this general approach is that by synthesizing the information coming from biochemical studies of this type, it should be possible to explain the function of prostaglandins in the intact kidney.
KeywordsAdenylate Cyclase Tubule Cell Renal Medulla Intracellular cAMP Level PGE2 Synthesis
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