Regulation of Prostaglandin Biosynthesis in Cultured Renal Medullary Interstitial Cells

  • Randall Mark Zusman


Advances in the understanding of arachidonic acid metabolism have led to the isolation and identification of the prostaglandins (PGs), thromboxanes, prostacyclin, and leukotrienes. It is now known that virtually every cell of mammalian organisms is capable of initiating the metabolism of arachidonic acid, which was originally thought to be a unique product of the seminal vesicle. In every cell type the initiation of the synthetic cascade involves the release of arachidonic acid from its cellular storage pool, predominantly phospholipids and triglycerides, and conversion to the prostaglandin endoperoxides PGG2 and PGH2 by the cyclooxygenase enzyme. The subsequent synthesis of prostaglandins E2 and F, thromboxane A2, or prostacyclin (PGI2) is dependent upon the presence or absence of the specific enzymes responsible for the conversion of PGG2 and PGH2 to the respective end products. Thus an understanding of the regulation of prostaglandin biosynthesis at the cellular level is dependent upon the study of the rate-limiting step in the biosynthetic cascade. This rate-limiting reaction is the release of arachidonic acid from the cellular storage pool. Once liberated from the complex lipids within the cellular matrix and cell wall, the free arachidonate enters the cytoplasm and is rapidly metabolized. An understanding of the factors which affect the rate of arachidonic acid release from the phospholipid—triglyceride storage pool must therefore be the focus of an analysis of the cellular regulation of prostaglandin biosynthesis.


Arachidonic Acid Angiotensin Converting Enzyme Inhibitor Phospholipase Activity Arachidonic Acid Release PGE2 Synthesis 
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Copyright information

© Plenum Publishing Corporation 1983

Authors and Affiliations

  • Randall Mark Zusman
    • 1
    • 2
  1. 1.Medical Services, Cardiac and Hypertension UnitsMassachusetts General HospitalBostonUSA
  2. 2.Department of MedicineHarvard Medical SchoolBostonUSA

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