Inheritance of Oligomycin Resistance in Tissue Culture Cells

  • Jerome M. Eisenstadt
  • Mary C. Kuhns


The mitochondrial ATPase complex has been resolved into the soluble F1, having the catalytic site for ATP synthesis, and the hydrophobic membrane-integrated F0, involved in proton translocation (Senior, 1973; Koslov and Skulacher, 1977). The F0 has been studied with the use of oligomycin and dicyclohexylcarbodiimide (DCCD), which inhibit enzyme activity of the complex by acting on a component of F0, (Bulos and Racker, 1968; Roberton et al., 1968; Sebald et al., 1976, 1979; Kiehl and Hatefi, 1980).


Mitochondrial Genome Neurospora Crassa Aspergillus Nidulans Tissue Culture Cell Cloning Efficiency 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Anderson, S., Bankier, A. T., Barrell, B. G., de Bruijn, M. H. L., Coulson, A. R., Drouin, J., Eperon, I. C., Nierlich, D. P., Roe, B. A., Sanger, F., Schreier, P. H., Smith, A. J. H., Staden, R., and Young, I. G., 1981, Sequence and organization of the human mitochondrial genome, Nature 290:457–465.PubMedCrossRefGoogle Scholar
  2. Avner, P. R., Coen, D., Dujon, B., and Slonimski, P. P., 1973, Mitochondrial genetics. IV. Allelism and mapping studies of oligomycin resistant mutants in S. cerevisiae, Mol. Gen. Genet. 125:9–52.PubMedCrossRefGoogle Scholar
  3. Breen, G. A. M., and Scheffler, I. E., 1980, Cytoplasmic inheritance of oligomycin resistance in Chinese hamster ovary cells, J. Cell. Biol. 86:723–729.PubMedCrossRefGoogle Scholar
  4. Bulos, B., and Racker, E., 1968, Partial resolution of the enzymes catalyzing oxidative phosphorylation, J. Biol. Chem. 243:3891–3900.PubMedGoogle Scholar
  5. Bunn, C. L., Wallace, D. C., and Eisenstadt, J. M., 1977, Cytoplasmic inheritance of chloramphenicol resistance in mouse tissue culture cells, Proc. Natl. Acad. Sci. USA 71:1681–1685.CrossRefGoogle Scholar
  6. Cattel, K. J., Knight, I. G., Lindop, G. R., and Beechey, R. B., 1970, The isolation of dicyclohexyl-carbodiimide binding proteins from mitochondrial membranes, Biochem. J. 117:1011–1013.Google Scholar
  7. Criddle, R. S., Arulanadan, C., Edwards, T., Johnson, R., Scharf, S., and Enns, R., 1979, Investigation of the oligomycin binding protein in yeast mitochondrial ATPase, in: Genetics and Biogenesis of Chloroplasts and Mitochondria (T. Bucher, ed.), Elsevier North-Holland, Amsterdam, pp. 151–157.Google Scholar
  8. Edwards, D. L., and Urger, B. W., 1978, Nuclear mutations conferring oligomycin resistance in Neurospora crassa, J. Biol. Chem. 253:4254–4258.PubMedGoogle Scholar
  9. Hensgens, L. A. M., Grivell, L. A., Borst, P., and Bos, J. L., 1979, Nucleotide sequence of the mitochondrial structural gene for subunit 9 of yeast ATPase complex, Proc. Natl. Acad. Sci. USA 765:1663–1667.CrossRefGoogle Scholar
  10. Howell, N., and Sager, R., 1979, Cytoplasmic genetics of mammalian cells: Conditional sensitivity to mitochondrial inhibitors and isolation of new mutant phenotypes, Somat. Cell Genet. 5:833–845.PubMedCrossRefGoogle Scholar
  11. Jackl, G., and Sebald, W., 1975, Identification of two products of mitochondrial protein synthesis associated with mitochondrial adenosine triphosphatase from Neurospora Crassa, Eur. J. Biochem. 54:97–106.PubMedCrossRefGoogle Scholar
  12. Kiehl, R., and Hatefi, Y., 1980, Interaction of [14C] dicyclohexylcarbodiimide with complex V (mitochondrial adenosine triphosphate synthetase complex), Biochemistry 19:541–548.PubMedCrossRefGoogle Scholar
  13. Koslov, I. A., and Skulacher, V. P., 1977, H ± Adenosine triphosphate and membrane energy coupling, Biochim. Biophys. Acta 463:29–89.Google Scholar
  14. Kuhns, M. C., and Eisenstadt, J. M., 1979, Oligomycin-resistant mitochondrial ATPase from mouse fibroblasts, Somat. Cell Genet. 5:821–832.PubMedCrossRefGoogle Scholar
  15. Kuhns, M. C., and Eisenstadt, J. M., 1981a, Nuclear inheritance of oligomycin resistance in mouse L cells, Somat. Cell Genet., 7:737–750.PubMedCrossRefGoogle Scholar
  16. Kuhns, M. C., and Eisenstadt, J. M., 1981b, A modified DCCD-binding protein in mouse fibroblast mutants with decreased sensitivity to oligomycin and dicychohexylcarbodiimide, Fed. Proc. 40:1277a.Google Scholar
  17. Kuhns, M. C., and Eisenstadt, J. M., 1982, The dicyclohexylcarbodiimide-binding protein of the mitochondrial ATPase from mouse L cells, submitted.Google Scholar
  18. Lagarde, A. E., and Siminovitch, L., 1979, Studies on Chinese hamster ovary mutants showing multiple cross-reference to oxidative phosphorylation inhibitors, Somat. Cell Genet. 5:847–871.PubMedCrossRefGoogle Scholar
  19. Lichtor, T., and Getz, G. S., 1978, Cytoplasmic inheritance of rutamycin resistance in mouse fibroblasts, Proc. Natl. Acad. Sci. USA 75:323–328.CrossRefGoogle Scholar
  20. Macino, G., Scazzocchio, C., Waring, R. B., McPhail Berks, M., and Davies, R. W., 1980, Conservation and rearrangement of mitochondrial structural gene sequences, Nature 288:404–406.PubMedCrossRefGoogle Scholar
  21. Pullman, M. E., Penefsky, H. S., Datta, A., and Racker, E., 1960, Partial resolution of the enzymes catalyzing oxidative phosphorylation, J. Biol. Chem. 235:3322–3329.PubMedGoogle Scholar
  22. Roberton, A. M., Holloway, C. T., Knight, I. G., and Beechey, R. B., 1968, Biochem. J. 108:444–456.Google Scholar
  23. Rowlands, R. T., and Turner, G., 1973, Nuclear and extranuclear inheritance of oligomycin resistance in Aspergillus nidulans, Mol. Gen. Genet. 126:201–216.PubMedCrossRefGoogle Scholar
  24. Sebald, W., and Wachter, E., 1978, Amino acid sequence of the putative protophore of the energy-transducing ATPase complex, in: Energy Conservation in Biological Membranes (G. Schäfer, and M. Klingenberg, eds), Springer-Verlag, New York, pp. 228–236.Google Scholar
  25. Sebald, W., Graft, T., and Wild, G., 1976, Cytoplasmic synthesis of the dicyclohexylcarbodiimidebinding protein in Meurospora Crassa, in: Genetics and Biogenesis of Chloroplasts and Mitochondria (T. Bucher, ed.), Elsevier North-Holland, Amsterdam, pp. 167–174.Google Scholar
  26. Sebald, W., Wachter, E., and Tzagoloff, A., 1979, Identification of amino acid substitutions in the dicyclohexylcarbodiimide-binding subunit of the mitochondrial ATPase complex from oligomycin-resistant mutants of Saccharomyces cerevisiae, Eur. J. Biochem. 100:559–607.CrossRefGoogle Scholar
  27. Senior, A. E., 1973, The structure of mitochondrial ATPase, Biochim. Biophys. Acta 301:249–277.PubMedGoogle Scholar
  28. Sierra, M. F., and Tzagoloff, A., 1973, Assembly of the mitochondrial membrane system. Purification of a mitochondrial product of the ATPase, Proc. Natl. Acad. Sci. USA 70:3155–3159.PubMedCrossRefGoogle Scholar
  29. Turner, G., Imam, G., and Küntzel, H., 1979, Mitochondrial ATPase complex of Aspergillus nidulans and the dicyclohexylcarbodiimide-binding protein, Eur. J. Biochem. 97:565–571.PubMedCrossRefGoogle Scholar
  30. Tzagoloff, A., Akai, A., and Needleman, R. B., 1975, Characterization of nuclear mutants of Saccharomyces cerevisiae with defects in mitochondrial ATPase and respiratory enzymes, J. Biol. Chem. 250:8228–8235.PubMedGoogle Scholar
  31. Wigler, M. H., and Weinstein, I. B., 1975, A preparative method for obtaining enucleated mammalian cells, Biochem. Biophys. Res. Commun. 63:669–674.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Jerome M. Eisenstadt
    • 1
  • Mary C. Kuhns
    • 1
  1. 1.Department of Human GeneticsYale University School of MedicineNew HavenUSA

Personalised recommendations