Nonselective Isolation of Fibroblast Heterokaryons, Hybrids, and Cybrids by Flow Sorting
The use of new cell combinations to study genetic interaction between somatic cells (e.g., fibroblast heterokaryons, hybrids, and cybrids) is hampered by the fact that no selection procedures are available for the short-term isolation of the specific fusion products. Moreover, the techniques for the isolation of cytoplasts (anucleate cells) and karyoplasts (minicells) [for a review see Ringertz and Savage (1976)] yield fractions which are contaminated considerably with nonenucleated cells. The use of these impure fractions as a fusing partner may give rise to difficulties in the interpretation of the results.
KeywordsFusion Product Xeroderma Pigmentosum Polystyrene Bead Tetraploid Cell Fluorescent Bead
Unable to display preview. Download preview PDF.
- Glick, D., 1961, Quantitative Chemical Techniques of Histo and Cytochemistry, John Wiley and Sons, New York.Google Scholar
- Jongkind, J. F., Verkerk, A., Schaap, G. H., and Galjaard, H., 1981, Flow-sorting in studies on metabolic and genetic interaction between human fibroblasts, Acta Path. Microhiol. Scand. (A) Suppl. 274:164–169.Google Scholar
- Jongkind, J. F., Verkerk, A., Visser, W. J., and Van Dongen, J. M., 1982, Isolation of autofluorescent “aged” human fibroblasts by flow sorting. Morphology, enzyme activity and proliferative capacity, Exp. Cell Res., (in press).Google Scholar
- Loewe, H., and Urbanietz, J., 1974, Basisch substituierte 2,6-Bis-benzimidanolderivate, eine neue chemotherapeutisch aktive Körperklasse, Arzneim. Forsch. 24:1927–1933.Google Scholar
- Lowry, O. H., and Passonneau, J. V., 1972, A Flexible System of Enzymatic Analysis, Academic Press, New York.Google Scholar
- Ringertz, N. R., and Savage, R. E, 1976, Cell Hybrids, Academic Press, New York.Google Scholar
- Schaap, G. H., Verkerk, A., Van der Kamp, A. W. M., Ory, F. G., and Jongkind, J. F., 1981, Separation of cytoplasts from nucleated cells by flow sorting using Hoechst 33342, Acta Patho. Microbiol. Scand. [A] Suppl. 274:159–163.Google Scholar
- Siniscalco, M., Klinger, H. P., Eagle, H., Koprowski, H., Fujimoto, W. Y., and Seegmiller, J. E., 1969, Evidence for intergenic complementation in hybrid cells derived from two human diploid strains each carrying an X-linked mutation, Proc. Natl. Acad. Sci. USA 62:793–799.PubMedCrossRefGoogle Scholar