Future Perspectives in Somatic Cell Genetics

  • Doris L. Slate
  • Frank H. Ruddle


Somatic cell genetic techniques have proven valuable in determining the arrangement of genes on chromosomes and in studying the regulation of eukaryotic gene expression. Studies with somatic cells taken from patients with inherited diseases have revealed the biochemical bases for many human disorders, and have provided potential strategies for diagnostic and therapeutic approaches. The generation of mutants or variants of somatic cells in culture can facilitate the analysis of complex physiologic systems via complementation analysis.


Gene Transfer Cell Hybrid Recipient Cell Mouse Mammary Tumor Virus Mediate Gene Transfer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. D’Eustachio, P., Pravtcheva, D., Marcu, K., and Ruddle, F. H., 1980, Chromosomal location of the structural gene cluster encoding murine immunoglobulin heavy chains, J. Exp. Med. 151:1545–1550.PubMedCrossRefGoogle Scholar
  2. Gordon, J. W., Scangos, G. A., Plotkin, D. J., Barbosa, J. A., and Ruddle, F. H., 1980, Genetic transformation of mouse embryos by microinjection of purified DNA, Proc. Natl. Acad. Sci. USA 77:7380–7384.PubMedCrossRefGoogle Scholar
  3. Harper, M. E., Ullrich, A., and Saunders, G. E., 1981, Localization of the human insulin gene to the distal end of the short arm of chromosome 11, Proc. Natl. Acad. Sci. USA 78:4458–4460.PubMedCrossRefGoogle Scholar
  4. Hynes, N. E., Kennedy, N., Rahmsdorf, U., and Groner, B., 1981, Hormone-responsive expression of an endogenous proviral gene of mouse mammary tumor virus after molecular cloning and gene transfer into cultured cells, Proc. Natl. Acad. Sci. USA 78:2038–2042.PubMedCrossRefGoogle Scholar
  5. Klobutcher, L. A., and Ruddle, F. H., 1979, Phenotype stabilization and integration of transferred material in chromosome mediated gene transfer, Nature 280:657–660.PubMedCrossRefGoogle Scholar
  6. Kurtz, D. T., 1981, Hormonal inducibility of rat α globulin genes in transfected mouse cells, Nature 291:629–631.PubMedCrossRefGoogle Scholar
  7. Owerbach, D., Rutter, W. J., Shows, T. B., Gray, P., Goeddel, D. V., and Lawn, R. M., 1981, Leucocyte and fibroblast interferon genes are located on human chromosome 9, Proc. Natl. Acad. Sci. USA 78:3123–3127.PubMedCrossRefGoogle Scholar
  8. Ringertz, N. R., and Savage, R. E., 1976, in: Cell Hybrids, Academic Press, New York.Google Scholar
  9. Ruddle, F. H., and Creagan, R. P., 1975, Parasexual approaches to the genetics of man, Ann. Rev. Genet. 9:407–486.PubMedCrossRefGoogle Scholar
  10. Schimke, R. T., Brown, P. C., Kaufman, R. J., McGrogan, M., and Slate, D. L., 1981, Chromosomal and extrachromosomal localization of amplified dihydrofolate reductase genes in cultured mammalian cells, Cold Spring Harbor Symp. Quant. Biol. 45:785–797.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Doris L. Slate
    • 1
  • Frank H. Ruddle
    • 1
  1. 1.Department of BiologyYale UniversityNew HavenUSA

Personalised recommendations