Viral-DNA Vectors in the Analysis of Mammalian Differentiation

  • Alban Linnenbach
  • Carlo M. Croce


Advances in molecular biology and methods in mammalian cell culture have been utilized in the construction of DNA-transformed, pluripotent murine teratocarcinoma stem cells (Linnenbach et al., 1980) that have proven to be a useful in vitro model for the study of the regulation of gene expression during differentiation (Huebner et al., 1981; Croce et al., 1981; Linnenbach et al., 1981).


Retinoic Acid Simian Virus Retinoic Acid Induction Plasmid Genome Mammalian Differentiation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Alwine, J. C., Kemp, D. J., Parker, B. A., Reiser, J., Renart, J., Stark, G. A., and Wahl, G. M., 1979, Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper, in: Methods in Enzymology, Volume 68 (R. Wu, ed.), Academic Press, New York, pp. 220–242.Google Scholar
  2. Aviv, H., and Leder, P., 1972, Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose, Proc. Natl. Acad. Sci. USA 69:1408–1412.PubMedCrossRefGoogle Scholar
  3. Bantle, J. A., Maxwell, I. H., and Hahn, W. E., 1976, Specificity of oligo (dT)-cellulose chromatography in the isolation of polyadenylated RNA, Anal. Biochem. 72:413–427.PubMedCrossRefGoogle Scholar
  4. Berger, S., and Birkenmeir, C., 1979, Inhibition of intractable nucleases with ribonucleosidevanadyl complexes: Isolation of messenger ribonucleic acid from resting lymphocytes, Biochemistry 18:5143–5149.PubMedCrossRefGoogle Scholar
  5. Berk, A. J., and Sharp, P. A., 1977, Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of Si endonuclease digested hybrids, Cell 12:721–732.PubMedCrossRefGoogle Scholar
  6. Berk, A. J., and Sharp, P. A., 1978, Spliced early mRNAs of simian virus 40, Proc. Natl. Acad. Sci. USA 75:1274–1278.PubMedCrossRefGoogle Scholar
  7. Bolivar, F., Rodriguez, R., Green, P., Betlach, M., Heyneker, H., Boyer, H., Crosa, I., and Falkow, S., 1977, Construction and characterization of new cloning vehicles. II. A multi-purpose cloning system, Gene 2:95–113.PubMedCrossRefGoogle Scholar
  8. Carmichael, G., and McMaster, G., 1980, The analysis of nucleic acids in gels using glyoxal and acridine orange, in: Methods in Enzymology, Volume 65 (L. Grossman and K. Maldowe, eds.), Academic Press, New York, pp. 380–391.Google Scholar
  9. Clewell, D. E., and Helsinki, D. R., 1960, Supercoiled DNA-protein complex in Escherichia Coli: Purification and induced conversion to an open circular DNA form, Proc. Natl. Acad. Sci. USA 62:1159–1166.CrossRefGoogle Scholar
  10. Croce, C. M., Linnenbach, A., Huebner, K., Parnes, J., Margulies, D., Appela, E., Seidman, J., 1981, Control of expression of histocompatibility antigens (H-2) and β2-microglobulin in F9 teratocarcinoma stem cells, Proc. Natl. Acad. Sci. USA 78:5754–5758.PubMedCrossRefGoogle Scholar
  11. Curtiss, R., III, Pereira, D. A., Hsu, J. C., Hull, S. C., Clark, J. F., Maturin, L. J., Sr., Goldschmidt, R., Moody, R., Inoue, M., and Alexander, L., 1977, Biological containment: The subordination of Escherichia coli K-12, in: Recombinant Molecules: Impact on Science and Society (R. F. Beers and E. G. Bassett, eds.), Raven Press, New York, pp. 45–56.Google Scholar
  12. Duesberg, P., and Vogt, P., 1973, Gel electrophoresis of avian leukosis and sarcoma viral RNA in formamide: Comparison with the other viral and cellular RNA species, J. Virol. 12:594–599.PubMedGoogle Scholar
  13. Favalora, J., Triesman, R., and Karmen, R., 1980, Transcriptional maps of polyoma virus-specific RNA: Analysis by two-dimensional nuclease S1 gel mapping, in: Methods in Enzymology, Volume 65 (L. Grossman and K. Maldowe, eds.), Academic Press, New York, pp. 718–749.Google Scholar
  14. Glisin, V., Crkvenjakov, R., and Byus, C., 1974, Ribonucleic acid isolation by cesium chloride centrifugation, Biochemistry 13:2633–2637.PubMedCrossRefGoogle Scholar
  15. Gmiir, R., Solter, D., and Knowles, B. B., 1980, Independent regulation of H-2K and H-2D gene expression in murine teratocarcinoma somatic cell hybrids, J. Exp. Med. 151:1349–1359.CrossRefGoogle Scholar
  16. Graham, F.L., and Van der Eb., A. J., 1973, Anew technique for the assay of infectivity of human adenovirus 5 DNA, Virology 52:456–567.PubMedCrossRefGoogle Scholar
  17. Grunstein, M., and Hogness, D. S., 1975, Colony hybridization: A method for the isolation of cloned DNAs that contain a specific gene, Proc. Natl. Acad. Sci. USA 72:3961–3965.PubMedCrossRefGoogle Scholar
  18. Hirt, B., 1967, Selective extraction of polyoma DNA from infected mouse cell cultures, J. Mol. Biol. 26:365–369.PubMedCrossRefGoogle Scholar
  19. Huebner, K., Linnenbach, A., Weidner, S., Glenn, G., and Croce, C. M., 1981, Deoxyribonuclease I sensitivity of plasmid genomes in teratocarcinoma-derived stem and differentiated cells, Proc. Natl Acad. Sci. USA 78:5071–5075.PubMedCrossRefGoogle Scholar
  20. Hughes, E. W., and August, T. T., 1981, Characterization of plasma membrane proteins identified by monoclonal antibodies, J. Biol. Chem. 256:664–671.PubMedGoogle Scholar
  21. Knowles, B. B., Pan, S. H., Solter, D., Linnenbach, A., Croce, C. M., and Huebner, K., 1980, Expression of H-2 laminin and SV40T and TASA on differentiation of transformed murine teratocarcinoma cells, Nature 288:615–618.PubMedCrossRefGoogle Scholar
  22. Laemmli, U. K., 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227:680–685.PubMedCrossRefGoogle Scholar
  23. Linnenbach, A., Huebner, K., and Croce, C. M., 1980, DNA-transformed murine teratocarcinoma cells: Regulation of expression of simian virus 40 tumor antigen in stem versus differentiated cells, Proc. Natl Acad. Sci. USA 77:4875–4879.PubMedCrossRefGoogle Scholar
  24. Linnenbach, A., Huebner, K., and Croce, C. M., 1981, Transcription of the simian virus 40 genome in DNA-transformed murine teratocarcinoma stem cells, Proc. Natl Acad. Sci. USA 78:6386–6390.PubMedCrossRefGoogle Scholar
  25. Linzer, D. I., and Levine, A. I., 1979, Characterization of a 54K Dalton cellular SV40 tumor antigen present in SV40 transformed cells and uninfected embryonal carcinoma cells, Cell 17:43–52.PubMedCrossRefGoogle Scholar
  26. Littlefield, J. W., 1965, The use of drug-resistant markers to study the hybridization of mouse fibroblasts, Exp. Cell Res. 41:190–196.CrossRefGoogle Scholar
  27. Maniatis, T., Kee, S. G., Efstratiadis, A., and Katatos, F. C., 1976, Amplification and characterization of a β-globin gene synthesized in vitro, Cell 8:163–182.PubMedCrossRefGoogle Scholar
  28. Martin, G., and Evans, M. J., 1975, Multiple differentiation of clonal teratocarcinoma stem cells following embryonal body formation in vitro, Cell 6:467–474.CrossRefGoogle Scholar
  29. Martinis, J., and Croce, C. M., 1978, Somatic cell hybrids producing antibodies specific for the tumor antigen of simian virus 40, Proc. Natl Acad. Sci. USA 75:2320–2323.PubMedCrossRefGoogle Scholar
  30. Maxam, A. M., and Gilbert, W., 1977, A new method for sequencing DNA, Proc. Natl Acad. Sci. USA 74:560–564.PubMedCrossRefGoogle Scholar
  31. McKnight, S., 1980, The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene, Nucleic Acids Res. 8:5949–5964.PubMedCrossRefGoogle Scholar
  32. McKnight, S., Croce, C. M., and Kingsbury, R., 1979, Introduction of isolated DNA sequences into cultured eukaryotic cells, Carnegie Institute Yearbook 78:56–61.Google Scholar
  33. Migeon, B., Smith, S. W., and Leddy, C., 1969, The nature of thymidine kinase in the humanmouse hybrid cell, Biochem. Genet. 3:583–590.PubMedCrossRefGoogle Scholar
  34. Pope, J. H., and Rowe, W. P., 1964, Detection of specific antigen in SV40 transformed cells by immunofluorescence, J. Exp. Med. 120:121–127.PubMedCrossRefGoogle Scholar
  35. Radloff, R., Bauer, W., and Vinograd, J., 1967, A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: The closed circular DNA in Hela cells, Proc. Natl. Acad. Sci. USA 57:1514–1522.PubMedCrossRefGoogle Scholar
  36. Scherrer, K., 1969, Isolation and sucrose gradient analysis of RNA, in: Fundamental Techniques in Virology (K. Habel and N. Salzman, eds.), Academic Press, New York, pp. 413–432.Google Scholar
  37. Segal, S., and Khoury, G., 1979, Differentiation as a requirement for simian virus 40 gene expression in F9 embryonal carcinoma cells, Proc. Natl. Acad. Sci. USA 76:5611–5616.PubMedCrossRefGoogle Scholar
  38. Southern, E., 1975, Detection of specific sequences among DNA fragments separated by gel electrophoresis, J. Mol. Biol. 98:503–517.PubMedCrossRefGoogle Scholar
  39. Spencer, N., Hopkinson, D. A., and Harris, H., 1964, Phosphoglucomutase polymorphism in man, Nature 204:742–745.PubMedCrossRefGoogle Scholar
  40. Strickland, S., and Mahdavi, V., 1978, The induction of differentiation in teratocarcinoma stem cells by retinoic acid, Cell 15:393–403.PubMedCrossRefGoogle Scholar
  41. Thomas, P., 1980, Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose, Proc. Natl. Acad. Sci. USA 77:5201–5205.PubMedCrossRefGoogle Scholar
  42. Tonegawa, S., Brack, C., Hozumi, N., and Schuller, R., 1977, Cloning of an immunoglobulin variable region gene from mouse embryo, Proc. Natl. Acad. Sci. USA 74:3518–3522.PubMedCrossRefGoogle Scholar
  43. Wigler, M., Silverstein, S., Lee, L., Pellicer, A., Cheng, Y., Axel, R., 1977, Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells, Cell 11:223–232.PubMedCrossRefGoogle Scholar
  44. Wigler, M., Pellicer, A., Silverstein, S., Axel, R., Urlaub, G., and Chasin, L., 1979, DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells, Proc. Natl. Acad. Sci. USA 75:1373–1375.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Alban Linnenbach
    • 1
  • Carlo M. Croce
    • 1
  1. 1.Wistar Institute of Anatomy and BiologyPhiladelphiaUSA

Personalised recommendations