Techniques for Decreasing the Toxicity of Polyethylene Glycol

  • W. Edward Mercer
  • Renato Baserga


In recent years the use of the chemical fusogen polyethylene glycol (PEG) in place of inactivated Sendai virus (Harris and Watkins, 1965) has greatly facilitated somatic cell fusion experiments (Pontecorvo, 1975; Davidson and Gerald, 1976a). There are, however, some inherent problems associated with PEG-induced cell fusion. One problem is that PEG will effectively induce cell-to-cell fusion only within a very narrow range of concentrations (Davidson et al., 1976b), and as the optimum concentration of 50–55% is approached the cytotoxic effects from exposure to the fusogen become significant.


Polyethylene Glycol Fusion Index Viability Index Hybrid Coloni Somatic Cell Fusion 
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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • W. Edward Mercer
    • 1
  • Renato Baserga
    • 1
  1. 1.Department of Pathology and Fels Research InstituteTemple University School of MedicinePhiladelphiaUSA

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