Techniques for Decreasing the Toxicity of Polyethylene Glycol
In recent years the use of the chemical fusogen polyethylene glycol (PEG) in place of inactivated Sendai virus (Harris and Watkins, 1965) has greatly facilitated somatic cell fusion experiments (Pontecorvo, 1975; Davidson and Gerald, 1976a). There are, however, some inherent problems associated with PEG-induced cell fusion. One problem is that PEG will effectively induce cell-to-cell fusion only within a very narrow range of concentrations (Davidson et al., 1976b), and as the optimum concentration of 50–55% is approached the cytotoxic effects from exposure to the fusogen become significant.
KeywordsPolyethylene Glycol Fusion Index Viability Index Hybrid Coloni Somatic Cell Fusion
Unable to display preview. Download preview PDF.
- Davidson, R. L., and Gerald, P. S., 1976a, Induction of mammalian somatic cell hybridization by polyethylene glycol, in: Methods in Cell Biology, Volume XV (D. M. Prescott, ed.), Academic Press, New York.Google Scholar
- Fan, V. S. C., McCammon, J. R., Ealy, G. T., and Burke, K. V., 1979, Process of membrane fusion, in: XIth International Congress of Biochemistry Abstracts, National Research Council of Canada, Ottawa, Ontario, p. 387.Google Scholar
- Mercer, W. E., and Schlegel, R. A., 1982, Cytoplasts can transfer factor(s) which stimulate quiescent fibroblasts to enter S-phase, J. Cell Physiol. 110:(in press).Google Scholar
- Phillips, H., 1973, Dye exclusion tests for cell viability, in: Tissue Culture: Methods and Application (P. E. Kruse and M. K. Patterson, eds.), Academic Press, New York, pp. 406–408.Google Scholar
- Ringertz, N. R., and Savage, R. E., 1976, Cell Hybrids, Academic Press, New York, Chapter 4, pp. 41–43.Google Scholar
- Schlegel, R. A., and Mercer, W. E., 1980, Red cell-mediated microinjection of quiescent fibroblasts, in: Introduction of Macromolecules into Viable Mammalian Cells (R. Baserga, C. Croce, and G. Rovera eds.), Alan R. Liss, New York, pp. 371–379.Google Scholar