Techniques of DNA-Mediated Gene Transfer for Eukaryotic Cells
DNA transformation in bacteria has permitted detailed genetic analysis as well as the implementation of recombinant DNA technology. The utility of manipulation and cloning of genes in bacteria is well recognized. This chapter will concern the manipulation and cloning of genes in eukaryotic systems ranging from yeast to animal systems. Gene manipulation utilizing eukaryotic cloning systems permits the analysis of regulation of eukaryotic genes in their native environment and offers certain advantages for the production of foreign gene products which may require post-transcriptional modifications which are indigenous to eukaryotic organisms.
KeywordsTransformation Efficiency Recipient Cell Cloning System Thymidine Kinase Gene Autonomous Replicate Sequence
Unable to display preview. Download preview PDF.
- Bollon, A. P., and Stauver, M., 1980, DNA transformation efficiency of various bacterial and yeast host-vector systems, J. Clin. Hematol Oncol. 10:39–48.Google Scholar
- Graham, F. L., Abrahams, P. J., Mulder, C., Heijneker, H. L., Warnaar, S. O., de Vries, F. A. J., Fiers, W., and van der Eb, A. J., 1974, Studies on in vitro transformation by DNA and DNA fragments of human adenoviruses and Simian Virus 40, Cold Spring Harbor Symp. Quant. Biol. 39:637–650.CrossRefGoogle Scholar
- Hohn, B., and Hinnen, A., 1980, Cloning with cosmids in E. coli and yeast, in: Genetic Engineering, Volume 2 (J. K. Setlow and A. Hollaender, eds.), Plenum Press, New York, pp. 169–183.Google Scholar
- Simm, G. K., and Augustin, A., 1981, Transfection of L-cells with cloned H-2 genes leads to expression of corresponding alloantigens, Nature (in press).Google Scholar
- Sweet, R., Jackson, J., Lowy, I., Ostrander, M., Pellicer, A., Roberts, J., Robins, D., Sim, G.-K., Wold, B., Axel, R., and Silverstein, S., 1981, The expression, arrangement, and rearrangement of genes in DNA-transformed cells, in: Genes Chromosomes and Neoplasia (F. E. Arrighi, P. N. Rao and E. Stubblefield, eds.), Raven Press, New York, pp. 205–219.Google Scholar