Techniques for Isolating Chromosome-Containing Minisegregant Cells

  • R. T. Johnson
  • A. M. Mullinger


In solid tumors in vivo cells frequently undergo spontaneous disintegration. This often takes the form of fragmentation of the cell body into a number of membrane-bound pieces which are subsequently removed by phagocytosis. The phenomenon has been given the name apoptosis and it is common enough to influence the kinetics of tumor growth (Kerr et al., 1972; Bird et al., 1976). Though apoptosis is particularly associated with malignant cells, similar behavior has been observed in embryogenesis and during changes in tissue growth rate (Kerr et al., 1972).


HeLa Cell Nitrous Oxide Cold Storage Mitotic Cell Gradient Fraction 
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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • R. T. Johnson
    • 1
  • A. M. Mullinger
    • 1
  1. 1.Department of ZoologyUniversity of CambridgeCambridgeEngland

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