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Monolayer Enucleation of Colcemid-Treated Human Cells and Polyethylene Glycol 400-Mediated Fusion of Microkaryoplasts (Microcells) with Whole Cells

  • Andrew H. CrenshawJr.
  • Leonard R. Murrell

Abstract

Modern somatic cell genetics began with the observation that hybridization can be achieved by cell to cell fusion in culture (Barski et al., 1961). In recent years, this new era in the study of gene expression has produced many advances in hybridization technology. Hybridization techniques can be grouped into two general classes: (1) whole genome transfer, involving cell to cell fusion, and (2) partial genome transfer, involving the introduction of genome fragments into whole cells. Mouse-human whole-cell hybridization has been used extensively for human gene mapping (Ruddle and Creagan, 1975; McKusick and Ruddle, 1977) and has relied on the fact that human chromosomes are spontaneously eliminated from these hybrids. Lengthy cultivation, recloning, and analysis are necessary before accurate chromosome assignment is possible (Weiss and Green, 1967).

Keywords

Mitotic Spindle Condensed Chromosome Cell Fragment Complete Growth Medium Rodent Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Andrew H. CrenshawJr.
    • 1
  • Leonard R. Murrell
    • 1
  1. 1.Department of AnatomyUniversity of Tennessee Center for the Health SciencesMemphisUSA

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