Isolation and Characterization of Mitoplasts
The discovery by Carter (1967) that cytochalasin B (CB), a metabolite produced by the fungus Helminthosporium dematiodeum, causes nuclear extrusion and spontaneous enucleation in a small proportion of treated cells has led to the development of a number of techniques to study nucleocyto-plasmic interactions, cytoplasmic inheritance, and the creation of reconstituted cells. CB has a profound effect on the morphology of mammalian cells in culture and is best known for blocking cytokinesis without affecting the karyokinesis or the nuclear division that usually leads to the formation of binucleate cells (Carter, 1967; Ladda and Estensen, 1970). However, the unique feature of CB, its induction of spontaneous nuclear extrusion, has been exploited by the cell biologist to the maximum extent.
KeywordsMitotic Cell Interphase Nucleus Mitotic Chromosome Interphase Cell Ficoll Gradient
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