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Production of Microcytospheres

  • Gerd G. Maul
  • Josef Weibel

Abstract

Cytoplasmic vesicles can be isolated by several methods which utilize agents with cross-linking capabilities, formaldehyde or glutaraldehyde (Scott et al., 1979). These partially fixed vesicles are unable to attach to a substrate. Since, in certain types of experiments, cytoplasmic vesicles containing only the active ribosomal components and a normal cell membrane are desirable, a method was needed to ensure minimal contamination of vesicles with other organelles. Cytochalasin B has been used in the past to break down the microfilaments associated with the cell membrane (Carter, 1967) and to enucleate cells (Prescott et al., 1971; Veomett et al., 1976; Wigler and Weinstein, 1975; Gopalakrishnan and Tompson, 1975). The resulting cyto-plasts can then be fused with cells or karyoplasts of different origin (Shay, 1977). However, these cytoplasts contain all of the cellular organelles. We describe here a procedure that is speedy, sterile, and applicable to a variety of experiments in which it is essential that cytoplasmic parts of one cell type containing only defined components are fused with other cells. These types of cytoplasmic vesicles are named microcytospheres.

Keywords

HeLa Cell Mitotic Cell Size Marker Plastic Dish Cytoplasmic Vesicle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Gerd G. Maul
    • 1
  • Josef Weibel
    • 1
  1. 1.Wistar Institute of Anatomy and BiologyPhiladelphiaUSA

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