Mitochondrial Influences in Hybrid Cells
The analysis of genetic mechanisms underlying phenotypic expression in somatic cells depends on techniques which can demonstrate the presence of particular heritable components. For genetic elements located in the nucleus of the cell, numerous chromosomal, drug resistance, and biochemical markers are now available which allow study of specific loci. Similarly, for the analysis of mitochondrial inheritance in somatic cells there has been increased availability of drug resistance markers which can be transmitted by cytoplasmic transfer (Bunn et al., 1974; Lictor and Getz, 1978; Harris, 1978). Specific differences in buoyant density and restriction endonuclease digest patterns of mitochondrial DNA also have served as markers for the identification of cytoplasmic genetic determinants (Dawid et al., 1974; Case and Wallace, 1981).
KeywordsHybrid Cell Somatic Cell Hybrid Chinese Hamster Cell Cytoplasmic Inheritance Mitochondrial Genetic
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