Selection of Purine and Pyrimidine Nucleoside Analog Resistance in Mammalian Cells
Since its inception, somatic cell genetics has taken advantage of purine and pyrimidine analog resistance. In the initial stages of the development of this discipline variants resistant to these substances represented the most important and technically useful markers available in permanent cultured cell lines. Although in the past decade many new selective characters have been exploited, these substances are still widely used in genetic studies on somatic cells (Table I). Investigations in experimental mutagenesis (Clive et al., 1972), studies on DNA-mediated transformation (Scangos and Ruddle, 1981), gene mapping (McKusick, 1980), transport (Wohlhueter et al., 1978), somatic recombination (Rosenstraus and Chasin, 1978), and gene inactivation (Bradley, 1979) have taken advantage of these markers (Morrow, 1982).
KeywordsThymidine Kinase Chinese Hamster Cell Phosphoribosyl Transferase Pyrimidine Nucleoside Somatic Cell Genetic
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