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The Molecular Nature of 1,25-(OH)2-D3-Induced Calcium-Binding Protein Biosynthesis in the Rat

  • Felix Bronner
  • Michael Buckley
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 151)

Abstract

Exogenous 1,25-(OH)2-D3, administered to vitamin D-replete animals on a high calcium diet, induces biosynthesis of the duodenal, cytosolic calcium-binding protein (CaBP) in less than 2 h. This process can be blocked by simultaneously administered cycloheximide, but not by actinomycin D. In vitamin D-replete animals on a low Ca diet, on the other hand, 1,25-(OH)2-D3 administration leads to new CaBP synthesis only after about 7 h; this process can be blocked by actinomycin D. In vitamin D-deficient animals on a high calcium diet who have no CaBP, treatment with 1,25-(OH)2-D3 induces CaBP formation in ≃ 8 h; this process is known to be blocked by actinomycin D. Thus in D-replete animals on a low calcium diet and in D-deficient animals, CaBP biosynthesis proceeds by a transcriptional route, whereas in D-replete animals on a high calcium diet the rapid response appears to be posttranscriptional. This finding points to the possibility of a more rapid regulatory action of vitamin D than previously reported and how vitamin D might function in the D-replete state.

Keywords

Calcium Diet Calcium Transport Other Hand High Calcium Diet High Phosphorus Diet 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Felix Bronner
    • 1
  • Michael Buckley
    • 1
  1. 1.Department of Oral Biology, School of Dental MedicineUniversity of Connecticut Health CenterFarmingtonUSA

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