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Development and Application of an Exchange Assay for 1,25-Dihydroxyvitamin D3

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Regulation of Phosphate and Mineral Metabolism

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 151))

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Abstract

1,25-Dihydroxyvitamin D3 (1,25(OH)2D3), an active metabolite of the secosteroid vitamin D, is now generally considered to be a steroid hormone by classical criteria (1). Our recent data indicate that unoccupied 1,25(OH)2D3 receptors are present in crude chromatin fractions under low salt conditions in vitro (2,3). Consequently, quantitation of unoccupied receptor levels in the chick intestinal mucosa is best achieved by assay of the crude chromatin fraction (4). Due to the slow dissociation rate of 1,25(OH)2D3 from its receptor (2), only endogenously unoccupied 1,25(OH)2D3 receptors are detected by this method (incubation with [3H]1,25(OH)2D3 at 4°C for 4 h).

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References

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Author information

Author notes

  1. Marian R. Walters

    Present address: Department of Physiology, Tulane University School of Medicine, New Orleans, USA

  2. Willi Hunziker

    Present address: Dept. of Pharmacology, School of Veterinary Medicine, University of Bern, Switzerland

  3. Arie Bar

    Present address: Volcani Center, Institute of Animal Sciences, Bet Dagan, Israel

Authors and Affiliations

  1. Department of Biochemistry, University of California, Riverside, California, USA

    Anthony W. Norman, Marian R. Walters, Willi Hunziker & Arie Bar

Authors
  1. Anthony W. Norman
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  2. Marian R. Walters
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  3. Willi Hunziker
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  4. Arie Bar
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Editor information

Editors and Affiliations

  1. University of Southern California, Los Angeles, California, USA

    Shaul G. Massry

  2. State University of New York at Stony Brook, Stony Brook, New York, USA

    Joseph M. Letteri

  3. Nassau County Medical Center, East Meadow, New York, USA

    Joseph M. Letteri

  4. University of Heidelberg, Heidelberg, Federal Republic of Germany

    Eberhard Ritz

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© 1982 Plenum Press, New York

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Norman, A.W., Walters, M.R., Hunziker, W., Bar, A. (1982). Development and Application of an Exchange Assay for 1,25-Dihydroxyvitamin D3 . In: Massry, S.G., Letteri, J.M., Ritz, E. (eds) Regulation of Phosphate and Mineral Metabolism. Advances in Experimental Medicine and Biology, vol 151. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4259-5_38

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  • DOI: https://doi.org/10.1007/978-1-4684-4259-5_38

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-4261-8

  • Online ISBN: 978-1-4684-4259-5

  • eBook Packages: Springer Book Archive

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