Development and Application of an Exchange Assay for 1,25-Dihydroxyvitamin D3

  • Anthony W. Norman
  • Marian R. Walters
  • Willi Hunziker
  • Arie Bar
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 151)


1,25-Dihydroxyvitamin D3 (1,25(OH)2D3), an active metabolite of the secosteroid vitamin D, is now generally considered to be a steroid hormone by classical criteria (1). Our recent data indicate that unoccupied 1,25(OH)2D3 receptors are present in crude chromatin fractions under low salt conditions in vitro (2,3). Consequently, quantitation of unoccupied receptor levels in the chick intestinal mucosa is best achieved by assay of the crude chromatin fraction (4). Due to the slow dissociation rate of 1,25(OH)2D3 from its receptor (2), only endogenously unoccupied 1,25(OH)2D3 receptors are detected by this method (incubation with [3H]1,25(OH)2D3 at 4°C for 4 h).


Intestinal Mucosa Receptor Level Total Receptor Exchange Assay Linear Regression Relationship 
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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Anthony W. Norman
    • 1
  • Marian R. Walters
    • 1
  • Willi Hunziker
    • 1
  • Arie Bar
    • 1
  1. 1.Department of BiochemistryUniversity of CaliforniaRiversideUSA

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