Measurement of Intracellular Inorganic Phosphate in Human Blood Red Cells, Leucocytes and Platelets
Inorganic phosphate (Pi) has long been known to play an important role in biological systems. It is essential for structural integrity of cells, for example, as a constituent of membrane phospholipids. It is also involved in a host of anabolic and catabolic processes. In glycolysis, Pi participates in the degradation of glucose and synthesis of ATP. Pi has an important role in the regulation of the activity of many enzyme systems involved in energy-transforming reactions. In erythrocytes, through its regulation of the levels of 2,3-DPG (2,3-diphosphoglycerate) and ATP, Pi is indirectly involved in the delivery of oxygen to tissues (1). In urine, Pi is the major buffer system. In hypophosphatemic states, phosphate deficiency leads to many metabolic disturbances, including myopathy, haemolysis and impaired phagocytosis (2).
KeywordsHigh Pressure Liquid Chromatography Particulate Fraction Electron Dense Granule Dihydrogen Orthophosphate High Pressure Liquid Chromatography Method
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- 3.R.J. Hall: An improved method for the micro determination of inorganic phosphate in small volumes of biological fluids. J.of Med.Lab.Technology. 20: 97–103, 1963.Google Scholar
- 5.H. Holmsen, H.J. Day and E. Storm: Adenine nucleotide metabolism of blood platelets. VI Subcellular localisation of nucleotide pools with different functions in the platelet release reaction. Biochemica et Biophysica Acta. 186, 254–266, 1969.Google Scholar