Towards Mapping Genes to Chromosomes Using Two Dimensional Gel Electrophoresis of Proteins and Somatic Cell Hybridization. Preliminary Studies

  • Rodrigo Bravo
  • Stephen J. Fey
  • Heather Macdonald-Bravo
  • Reinhold Shäfer
  • Klaus Willecke
  • Julio E. Celis
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 50)


Fusion of somatic cells followed by loss of chromosomes from one of the parents represents a unique tool to assign phenotypic traits to particular chromosomes or to regions of chromosomes(1–7). This approach, however, is limited by the small number of genetic markers available for the selection of hybrids(8) and more seriously by the lack of sensitive assays to determine how much information is coexpressed in the hybrids at the molecular level(6, 7, 9–13).


Cell Hybrid Mouse Chromosome Original Number Hypoxanthine Phosphoribosyltransferase Natural Science Research Council 
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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Rodrigo Bravo
    • 1
  • Stephen J. Fey
    • 1
  • Heather Macdonald-Bravo
    • 1
  • Reinhold Shäfer
    • 2
  • Klaus Willecke
    • 2
  • Julio E. Celis
    • 1
  1. 1.Division of Biostructural Chemistry, Department of ChemistryAarhus UniversityAarhus CDenmark
  2. 2.Intitut für ZellbiologieUniversität EssenEssen 1Germany

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