Use of Somatic Cell Hybridization and DNA-Mediated Gene Transfer for Characterization of Neoplastic Transformation

  • Klaus Willecke
  • Reinhold Schäfer
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 50)


Somatic cell hybridization has been used with great success for the assignment of genes to human or mouse chromosomes (1). In most cases, phenotypes have been correlated with single genes on chromosomes. Complex phenotypes “which are caused by the expression of several different genes are difficult to dissect genetically by the standard methods of somatic cell genetics without additional use of techniques of molecular biology. It appears that neoplastic transformation is such a complex pheno type. The expression of malignancy in somatic cell hybrids has been investigated already in one of the erliest publications describing the cell fusion phenomenon (2). Since then many different cell systems and expression of many different phenotypes of neoplastic transformation have been studied in somatic cell hybrids. In particular, the question of dominant versus recessive expression of malignancy in cell hybrids has led to many controversial discussions in the literature. Very recently, the technique of DNA-mediated gene transfer has been successfully applied to the transfer of genes (or DNA sequences) involved in tumorigenesis.


Cell Hybrid Neoplastic Transformation Mouse Chromosome Somatic Cell Hybrid Chinese Hamster Cell 
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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Klaus Willecke
    • 1
  • Reinhold Schäfer
    • 1
  1. 1.Institut für Zellbiologie- (Tumorforschung)Universität EssenEssen 1Federal Republic of Germany

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