Abstract
Somatic cell hybridization has been used with great success for the assignment of genes to human or mouse chromosomes (1). In most cases, phenotypes have been correlated with single genes on chromosomes. Complex phenotypes “which are caused by the expression of several different genes are difficult to dissect genetically by the standard methods of somatic cell genetics without additional use of techniques of molecular biology. It appears that neoplastic transformation is such a complex pheno type. The expression of malignancy in somatic cell hybrids has been investigated already in one of the erliest publications describing the cell fusion phenomenon (2). Since then many different cell systems and expression of many different phenotypes of neoplastic transformation have been studied in somatic cell hybrids. In particular, the question of dominant versus recessive expression of malignancy in cell hybrids has led to many controversial discussions in the literature. Very recently, the technique of DNA-mediated gene transfer has been successfully applied to the transfer of genes (or DNA sequences) involved in tumorigenesis.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Ringertz, R.N. and Savage, R.E. (1976). Gene mapping and gene complementation analysis. In: Cell hybrids. Academic Press, New York, p. 224.
Barski, G., Sorieul, S., and Cornefert, F. (1961). “Hybrid” type cells in combined cultures of two different mammalian cell strains. J. Natl. Canc. Inst., 26, 1269.
Ozer, H.L. and Jha, K.K. (1977). Malignancy and trans-formation. Expression in somatic cell hybrids and variants. Adv. Canc. Res., 25, 53.
Barski, G. and Belehradek, Jr. J. (1979). Inheritance of malignancy in somatic cell hybrids. Somatic Cell Genet., 5, 897.
Harris, H. (1979). Some thoughts about genetics, differentiation, and malignancy. Somatic Cell Genet., 5, 923.
Sidebottom, E. (1980). The analysis of malignancy by cell fusion. Invitro (Rockville), 16, 77.
Croce, C.M. (1980). Cancer genes in cell hybrids. Biochim. Biophys. Acta, 605, 411.
Smets, L.A. (1980). Cell transformation as a model for tumor induction and neoplastic growth. Biochim. Biophys. Acta, 605, 93.
Harris, H. (1979). Some recent progress in the analysis of malignancy by cell fusion. In: The Ciba Foundation Symposion No. 66, 1978. Human Genetics: Possibilities and Realities (eds., R. Porter and M. O’Connor), Excerpta Medica, Amsterdam, p. 311.
Bishr Omary, M., Townbridge, I.S., and Minozada, J. (1980). Human cell-surface glycoprotein with unusual properties. Nature, 286, 888.
Kucherlapati, R. and Tepper, R. (1978). Modulation and mapping of human plasminogen activator by cell fusion. Cell, 15, 1331.
Eun, C.K. and Klinger, H.P. (1980). Human chromosome 11 affects the expression of fibronectin fibers in human x mouse cell hybrids. Cytogenet. Cell Genet., 27, 57.
Marshall, C.J. and Dave, H. (1978). Suppression of the transformed phenotype in somatic cell hybrids. J. Cell Science, 33, 171.
Schäfer, R., Doehmer, J., Driige, P.M., Rademacher, I., and Willecke, K. (1981). Genetic analysis of transformed and malignant phenotypes in somatic cell hybrids between tumorigenic Chinese hamster cells and diploid mouse fibroblasts. Cancer Res., 41, 1214.
Stanbridge, E.J. and Wilkinson, J. (1978). Analysis of malignancy in human cells: malignant and transformed phenotypes are under separate genetic control. Proc. Natl. Acad. Sci. U.S.A., 75., 1466.
Klinger, H.P. (1980). Suppression of tumorigenicity in somatic cell hybrids. I. Suppression and reexpression of tumorigenicity in diploid human x D98AH2 hybrids and independent segregation of tumorigenicity from other cell phenotypes. Cytogenet. Cell Genet., 27, 254.
Muggleton-Harris, A.L. and Palumbo, M. (1979). Nueleocytoplasmic interactions in experimental binucleates formed from normal and transformed components. Somatic Cell Genet., 5, 397.
Bunn, C.L. and Tarrant, G.M. (1980). Limited lifespan in somatic cell hybrids and cybrids. Exp. Cell Res., 127, 385.
Jonasson, J., Povey, S., and Harris, H. (1977). The analysis of malignancy by cell fusion. VII. Cytogenetic analysis of hybrids between malignant and diploid cells and of tumours derived from them. J. Cell Science, 24, 217.
Aviles, D., Jami, J., Rousset, J.-P., and Ritz, E. (1977). Tumor x host cell hybrids in the mouse: Chromosomes from the normal cell parent maintained in malignant hybrid tumors. J. Natl. Canc. Inst., 58, 1391.
Aviles, D., Ritz, E., and Jami, J. (1980). Chromosomes in tumors derived from mouse x diploid cell hybrids obtained in vitro. Somatic Cell Genet., 6, 171.
Kucherlapati, R. and Shin, S. (1979). Genetic control of tumorigenicity in interspecific mammalian cell hybrids. Cell, 16, 639.
Jonasson, J. and Harris, H. (1977). The analysis of malignancy by cell fusion. VIII. Evidence for the intervention of an extra-chromosomal element. J. Cell Science, 24, 255.
Howell, A.N. and Sager, R. (1978). Tumorigenicity and its suppression in cybrids of mouse and Chinese hamster cell lines. Proc. Natl. Acad. Sci. U.S.A., 75 2358.
Stiles, C.D., Chuman, L.M., and Saier, M.H. Jr. (1976). Enhancement of tumorigenicity in athymic nude mice by coinjection of tumor cells and embryonic fibroblasts. J. Cell Biol., 70, 169a.
Yamamoto, T., Rabinowitz, Z., and Sachs, L. (1973). Identification of the chromosomes that control malignancy. Nature New Biol., 243., 247.
Shih, C., Shilo, B.-Z., Goldfarb, M., Dannenberg, A., and Weinberg, R.A. (1979). Passage of phenotypes of chemically transformed cells via transfection of DNA and chromatin. Proc. Natl. Acad. Sci. U.S.A., 76, 5714.
Hill, M. and Hillowa, J. (1972). Virus recovery in chicken cells tested with Rous sarcoma cell DNA. Nature lew Biol., 237, 35.
Graham, F.L. (1977). Biological activity of tumor virus DNA. Adv. Canc. Res., 25, 1.
Cooper, G.M., Okenquist, S., and Silverman, L. (1980). Transforming activity of DNA of chemically transformed and normal cells. Nature, 284, 418.
Shih, C., Padhy, L.C., Murray, M., and Weinberg, R.A. (1981). Transforming genes of carcinomas and neuroblastomas introduced into mouse fibroblasts. Nature, 290, 261.
Krontiris, T.G. and Cooper, G.M. (1981). Transforming activity of human tumor DNAs. Proc. Natl. Acad. Sci. U.S.A., 78, 1181.
Jelinek, W.R., Toomey, T.P., Leinwand, L., Duncan, C.H., Biro, P.A., Choudary, P.V., Weissman, S.M., Rubin, C.M., Houck, C.M., Deininger, P. L., and Schmid, C.W. (1980). Ubiquitous, interspersed repeated sequences in mammalian genomes. Proc. Natl. Acad. Sci. U.S.A., 77, 1398.
Shilo, B.Z. and Weinberg, R.A. (1981). Unique transforming gene in carcinogen-transformed mouse cells. Nature, 289, 607.
Collett, M.S., Brugge, J.S., and Erickson, R.L. (1978). Characterization of a normal avian cell protein related to the Avian Sarcoma Virus transforming gene product. Cell, 15, 1363.
Hayward, W.S., Neel, B.G., and Astrin, S.M. (1981). Activation of a cellular onc gene by promotor insertion in ALV-induced lymphoid leucosis. Nature, 290, 475.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1982 Plenum Press, New York
About this chapter
Cite this chapter
Willecke, K., Schäfer, R. (1982). Use of Somatic Cell Hybridization and DNA-Mediated Gene Transfer for Characterization of Neoplastic Transformation. In: Caskey, C.T., Robbins, D.C. (eds) Somatic Cell Genetics. NATO Advanced Study Institutes Series, vol 50. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4256-4_11
Download citation
DOI: https://doi.org/10.1007/978-1-4684-4256-4_11
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-4258-8
Online ISBN: 978-1-4684-4256-4
eBook Packages: Springer Book Archive