Cell Hybridization: A Tool for the Study of Cell Differentiation

  • Mary C. Weiss
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 50)


One of the many uses of somatic cell hybridization has been to explore the kinds of regulatory mechanisms responsible for the acquisition and the maintenance of the differentiated state. When this work was begun in the late sixties, it was believed that the genetic analysis of cell differentiation, achieved by analyzing the properties of hybrid cells resulting from the fusion of various kinds of differentiated cells, would make it possible to deduce the kinds of genetic mechanisms responsible for the expression of tissue-specific genes. Since that time, it has become clear that regulation in mammalian cells is far too complex to be dissected by this kind of genetic approach alone. Nevertheless, the results obtained do make it possible to eliminate some kinds of models of cell differentiation. In addition, these studies have revealed that fundamentally different mechanisms are involved in the heritable potential of cells to express tissue specific genes, and in the actual expression of these genes.


Somatic Hybrid Hybrid Cell Somatic Cell Hybrid Parental Genome Gene Dosage Effect 
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  1. Allan, M. and Harrison, P., 1980, Co-expression of differentiation markers in hybrids between Friend cells and lymploid cells and the influence of cell shape. Cell 19:437–447.PubMedCrossRefGoogle Scholar
  2. Axelrod, D.E., Gopalakrishnan, T.V., Willing, M. and Anderson, W.F., 1978, Maintenance of hemoglobin inducibility in somatic cell hybrids of tetraploid (2s) mouse erythroleukemia cells with mouse or human fibroblasts. Somat. Cell Genet. 4:157–168.PubMedCrossRefGoogle Scholar
  3. Bertolotti, R., 1977, Expression of differentiated functions in hepatoma cell hybrids: selection in glucose-free media of hybrid cells which reexpress gluconeogenic enzymes. Somat. Cell Genet. 3:579–602.PubMedCrossRefGoogle Scholar
  4. Cassio, D., Hassoux, R., Dupiers, M., Uriel, J. and Weiss, M.C., 1980, Coordinate secretion of mouse alpha-fetoprotein, mouse albumin and rat albumin in mouse hepatoma-rat hepatoma hybrids. J. Cell Physiol. 104:295–308.PubMedCrossRefGoogle Scholar
  5. Cassio, D. and Weiss, M.C., 1979, Expression of fetal and neonatal hepatic functions by mouse hepatoma-rat hepatoma hybrids. Somat. Cell Genet. 5:719–738.PubMedCrossRefGoogle Scholar
  6. Davidson, R.L., 1974, Gene expression in somatic cell hybrids. Annu. Rev. Genetics 8:195–218.CrossRefGoogle Scholar
  7. Davis, F.M. and Adelberg, A., 1973, Use of somatic cell hybrids for analysis of the differentiated state. Bacteriol. Rev. 27:197–214.Google Scholar
  8. Deschatrette, J., Moore, E.E., Dubois, M., Cassio, D. and Weiss, M.C., 1979, Dedifferentiated variants of a rat hepatoma: analysis by cell hybridization. Somat. Cell Genet. 5:697–718.PubMedCrossRefGoogle Scholar
  9. Ephrussi, B., 1972, “Hybridization of somatic cells”. Princeton University Press, Princeton, NJ.Google Scholar
  10. Forquignon, F. and Ephrussi, B., 1979, Isolation and properties of amelanotic variants of a hamster melanoma. Somat. Cell Genet. 5:409–426.CrossRefGoogle Scholar
  11. Fougère, C. and Weiss, M.C., 1978, Phenotypic exclusion in mouse melanoma-rat hepatoma hybrid cells: pigment and albumin production are not reexpressed simultaneously. Cell 15:843–854.PubMedCrossRefGoogle Scholar
  12. Gopalakrishnan, T.V. and Anderson, W.F., 1979, Epigenetic activation of phenylalanine hydroxylase in mouse erythroleukemia cells by the cytoplast of rat hepatoma cells. Proc. Natl. Acad. Sci. USA76:3932–3936.PubMedCrossRefGoogle Scholar
  13. Gopalakrishnan, T.V., Thompson, E.B. and Anderson, W.F., 1977, Extinction of hemoglobin inducibility in Friend erythroleukemia cells by fusion with cytoplasm of enucleated mouse neuroblastoma or fibroblast cells. Proc. Natl. Acad. Sci. USA74:1642–1646.PubMedCrossRefGoogle Scholar
  14. Kahn, C.R., Bertolotti, R., Ninio, M. and Weiss, M.C., 1981, Shortlived cytoplasmic regulators of gene expression in cell cybrids. Nature290:717–720.PubMedCrossRefGoogle Scholar
  15. Linder, S., Brzeski, H. and Ringertz, N.R., 1979, Phenotypic expression in cybrids derived from teratocarcinoma cells fused with myoblast cytoplasms. Exp. Cell Res. 120:1–14.PubMedCrossRefGoogle Scholar
  16. Lipsch, L.A., Kates, J.R. and Lucus, J.J., 1979, Expression of a liver-specific function by mouse fibroblast nuclei transplanted into rat hepatoma cytoplasts. Nature 281:74–76.CrossRefGoogle Scholar
  17. McBurney, M.W., Featherstone, M.S. and Kaplan, H., 1978, Activation of teratocarcinoma-derived hemoglobin genes in teratocarcinoma-Friend cell hybrids. Cell 15:1323–1330.PubMedCrossRefGoogle Scholar
  18. McBurney, M.W. and Strutt, B., 1979, Fusion of embryonal carcinoma cells to fibroblast cells, cytoplasts and karyoplasts: developmental properties of viable fusion products. Exp. Cell Res. 124:171–180.PubMedCrossRefGoogle Scholar
  19. Mevel-Ninio, M. and Weiss, M.C., 1981, Immunofluorescent analysis of the time course of extinction, reexpression and activation of albumin production in heterokaryons and hybrids of rat hepatoma cells with mouse fibroblasts. J. Cell Biol., in press.Google Scholar
  20. Miller, R.A. and Ruddle, F.H., 1976, Pluripotent teratocarcinoma thymus somatic cell hybrids. Cell 9:45–55.PubMedCrossRefGoogle Scholar
  21. Miller, R.A. and Ruddle, F.H., 1977, Teratocarcinoma x Friend erythroleukemia cell hybrids resemble their pluripotent embryonal carcinoma parent. Dev. Biol. 56:157–173.PubMedCrossRefGoogle Scholar
  22. Ringertz, N.R., Krondahl, V. and Coleman, J.R., 1978, Reconstitution of cells by fusion of cell fragments. I. Myogenic expression after fusion of minicells from rat myoblasts (L6) with mouse fibroblast (A9) cytoplasm. Exp. Cell Res. 113:233–246.PubMedCrossRefGoogle Scholar
  23. Ringertz, N. and Savage, R.E., 1976, “Cell hybrids”. Academic Press, New York.Google Scholar
  24. Weiss, M.C., 1977, The use of somatic cell hybridization to probe the mechanisms which maintain cell differentiation. In “Human Genetics”, A. Armendares and R. Liskey, eds., Excerpta Medica, Amsterdam, 284–292.Google Scholar
  25. Willing, M.C., Nienhuis, A.W. and Anderson, W.F., 1979, Selective activation of human β- but not γ-globin gene in human fibroblast x mouse erythroleukemia cell hybrids. Nature 277:534–538.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Mary C. Weiss
    • 1
  1. 1.Centre de Génétique Moléculaire du C.N.R.S.Gif-Sur-YvetteFrance

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