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Liposome-Mediated DNA Transfer in Eukaryotic Cells: Gene Uptake and Expression in the Host Cell

  • Claude Nicolau
  • Claude Sené
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 47)

Abstract

Liposomes have been used to introduce drugs (Poste and Papahadjopoulos, 1976), enzymes (Gregoriadis and Buckland, 1973), mRNA (Dimitriadis, 1978), viruses (Lonberg-Holm et al., 1976; Wilson et al., 1977), and allosteric effectors (Nicolau and Gersonde, 1979; Gersonde and Nicolau, 1979) in a number of different cells. Recently, chromosomes have been entrapped in liposomes and transferred to cells (Mukherjee et al., 1978) and DNA has been introduced into bacterial cells (Fraley et al., 1979) and into plant cells (Lurquin, 1979). Also the expression of the tetracycline resistance gene (TetR) has been observed in E. coli after liposome-mediated transfer of the plasmid pBR322 to these cells (Fraley et al., 1979). Fraley et al. (1981) have used liposomes to encapsulate with high efficiency DNA isolated from SV40 virus. Infection of a permissive monkey cell line with these DNA-loaded liposomes resulted in the expression of the SV40 DNA encapsulated in liposomes with efficiencies comparable to the calcium phosphate method. Wong et al. (1980) isolated, from E. coli a restriction fragment, 875 bp, which encodes for a β-lactamase activity, and entrapped it in liposomes. Incubation of the DNA-loaded liposomes with avian, murine and human cells resulted in the uptake by the cells of the DNA and the expression of a β-lactamase activity as determined by spectroscopic and microbiological methods. Recently, Schaeffer-Ridder et al. (1981) transformed mouse LMTK cells with the liposome-encapsulated thymidine kinase gene and Nicolau and Rottem (1981) transformed M. capriolum with the pBR322 plasmid encapsulated in liposomes, conferring thus tetracycline resistance to this tetracycline sensitive organism. We will discuss here several aspects of the transformation of eukaryotic cells with liposome encapsulated genetic material.

Keywords

Hela Cell Lipid Vesicle pBR322 Plasmid Liposome Suspension Tetracycline Resistance Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Deamer, D. and Bangham, A.C., 1976, Large volume liposomes by an ether vaporization method, Biochim, Biophys, Acta, 443: 629.CrossRefGoogle Scholar
  2. Dimitriadis, G.T., 1978, Translation of rabbit globin mRNA intro-duced by liposomes into mouse lymphocytes, Nature, 274: 923.PubMedCrossRefGoogle Scholar
  3. Fraley, R.T., Fornari, C.S. and Kaplan, S., 1979, Entrapment of a bacterial plasmid in phospholipid vesicles: Potential for gene ransfer, Proc. Natl. Acad. Sci. USA, 76: 3348.PubMedCrossRefGoogle Scholar
  4. Gersonde, K. and Nicolau, C., 1979, Improvement of the human red blood cell 02 release capacity by lipid vesicle-mediated incorporation of inositol hexaphosphate, Blut, 39: 1.PubMedCrossRefGoogle Scholar
  5. Gregoriadis, G. and Buckland, R.A., 1973, Enzyme-containing liposomes alleviate a model for storage disease. Nature, 244: 170.PubMedCrossRefGoogle Scholar
  6. Loening, V.E., 1967, The fractionation of high-molecular weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem. J. 102: 251.PubMedGoogle Scholar
  7. Lonberg-Holm, J., Gosser, L.B. and Shimshick, E.J., 1976, Interaction of liposomes with subviral particles of poliovirus type 2 and rhinovirus type 2, J. Virol., 19: 746.PubMedGoogle Scholar
  8. Lurquin, P.F., 1979, Entrapment of plasmid DNA by liposomes and heir interactions with plant protoplasts, Nucl. Acids Res., 6: 3773.PubMedCrossRefGoogle Scholar
  9. Mukherjee, A.B., Orloff, S., Butler, J.D., Triche, T., Lalley, P. and Schulman, J.D., 1978, Entrapment of metaphase chromosomes into phospholipid vesicles (lipochromosomes): arrier potential in gene transfer, Proc. Natl. Acad. Sci. USA, 75: 1361.PubMedCrossRefGoogle Scholar
  10. Nicolau, C. and Gersonde, K., 1979, Incorporation of inositol hexa-phosphate into intact red blood cells, I. Fusion of effector-containing lipid vesicles with erythrocytes. Naturwissenschaften 66: 561.CrossRefGoogle Scholar
  11. Nicolau, C. and Send’, C., 1981, Cell-cycle dependence of the liposome mediated gene uptake by eukaryotic cells. Submitted for publication.Google Scholar
  12. Nicolau, C. and Rottem, S., 1981, Expression of TetR in M. capriolum fter liposome-mediated transfer of the pBR322 plasmid. In preparation.Google Scholar
  13. O’Callaghan, C.H., Morris, A., Kirby, S.M. and Shingler, A.H., 1972, Novel method for detection of β-lactamase by using a chromogenic cephalosporin substrate. Antimicrob. Agents Chemother., 1: 283.PubMedGoogle Scholar
  14. Poste, G. and Papahadjopoulos, D., 1976a, Lipid vesicles as carriers or introducing materials into cultured cells: Influence of vesicle lipid composition on mechanism of vesicle incorporation into cells, Proc. Natl. Acad. Sci. USA, 73: 1603.PubMedCrossRefGoogle Scholar
  15. Poste, G. and Papahadjopoulos, D., 1976b, Drug-containing lipid vesicles render drug-resistant tumour cells sensitive to actinomycin D, Nature, 261: 699.PubMedCrossRefGoogle Scholar
  16. Poste, G., Papahadjopoulos, D. and Vail, W., 1976, Lipid vesicles as arriers for introducing biologically active materials into cells, in: “Methods in Cell Biology”, vol. 14, D.M. Prescott, ed., Academic Press, New York.Google Scholar
  17. Schaefer-Ridder, M., Wang, Y. and Hofschneider, P.H., 1981, Liposomes as gene carriers: High efficiency transformation of mouse L cells by the thymidine kinase gene, Science (in press).Google Scholar
  18. Sené, C. and Nicolau, C., 1981, Liposome-mediated gene transfer in eukaryotic cells in: “Liposome, drugs and Immunocompetent ell Functions”, C. Nicolau and A. Paraf, ed., Academic Press, London.Google Scholar
  19. Singleton, W.S., Gray, M.S., Brown, M.L. and White, J.L., 1965, Chromatographically homogeneous lecithin from egg phospholipids, J. Am. Oil Chem. Soc., 42: 53.PubMedCrossRefGoogle Scholar
  20. Szoka, F., Jacobson, K., Derzko, Z. and Papahadjopoulos, D., 1980, Fluorescence studies on the mechanism of liposome-cell interactions in vitro, Biochim. Biophys. Acta, 600: 1.PubMedCrossRefGoogle Scholar
  21. Wilson, T., Papahadjopoulos, D. and Taber, R., 1977, Biological properties of poliovirus encapsulated in lipid vesicles: antibody resistance and infectivity in virus-resistant cells. Proc. Natl. Acad. Sci. USA, 74: 3471.PubMedCrossRefGoogle Scholar
  22. Wong, T.K., Nicolau, C. and Hofschneider, P.H., 1980, Appearance of actamase activity in animal cells upon liposome-mediated ene transfer, Gene, 10: 87.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Claude Nicolau
    • 1
    • 2
  • Claude Sené
    • 1
    • 2
  1. 1.Centre de Biophysique MoleculaireC.N.R.S.Orléans CedexFrance
  2. 2.Max Planck Institut für StrahlenchemieMülheim a.d. RuhrGermany

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