Image Reconstruction from Electron Micrographs of Macromolecular Structures
Knowledge of the three dimensional spatial organisation of a complex biological system is generally essential, if an understanding of its working is to be achieved. The assembly of viral capsids, the functioning of a ribosome in synthesising a protein, the repetitive molecular movements generating force in muscular contraction or the operation of the various cellular organelles involved in cell division are all critically dependent on the specific structures involved. Electron microscopy provides one approach to investigating such structures and this article outlines the procedures used to reconstruct reliable two and three dimensional images from the resulting micrographs. The main emphasis however is on the sort of structural information that can be derived.
KeywordsPurple Membrane Dimensional Reconstruction Nucleosome Core Histone Octamer Tomato Bushy Stunt Virus
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