Receptor Measurement

  • Ernest J. PeckJr.
  • Katrina L. Kelner


Many cellular activities are controlled via signals from outside the cell. Examples of extracellular signals include hormones and neurotransmitters which act on target cells by way of receptors to induce specific responses. Cells unresponsive to a given signal are nontargets and generally possess few, if any, receptors for that signal. Receptors are macromolecules that may exist as free and soluble components of the cytoplasm, may reside within various intracellular compartments, or may exist primarily on the outer surface of plasma membranes. Receptors function via their capacity to recognize and bind specific signals, i.e., ligands. Implicit in receptor theory is the assumption that the occupied receptor, i.e., receptor-ligand complex, is responsible for the production of a biological response. Although the correlation of receptor occupancy with biological response is an important issue in receptor studies, the assessment of receptor-ligand interactions is the primary subject of this chapter. We have recently reviewed the receptor occupancy/response relationship for steroid1 and neurotransmitter2 systems.


Nonspecific Binding Free Ligand Receptive Site Junctional Complex Scatchard Plot 
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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Ernest J. PeckJr.
    • 1
  • Katrina L. Kelner
    • 1
  1. 1.Department of Cell BiologyBaylor College of Medicine, Texas Medical CenterHoustonUSA

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