High-Performance Liquid Chromatography
High-performance liquid chromatography (HPLC) has become a standard technique in the laboratory. The intent of this chapter is to present an overview of the technical aspects of HPLC rather than an in-depth discussion. For more detailed information on HPLC, the reader is referred to the recently revised text by Synder and Kirkland.1 There is an extensive literature in the scientific journals covering the HPLC of virtually every class of compound, and it should be consulted by the reader for his own applications. Furthermore, manufacturers of HPLC equipment can be helpful in obtaining information about specific applications.
KeywordsBasic Amino Acid HPLC Column Pentapeptide Sequence Postcolumn Reaction Opiate Receptor Binding
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- 1.Snyder, L. R., and Kirkland, J. J., 1979, Introduction to Modern Liquid Chromategraphy, 2nd ed., John Wiley & Sons, New York.Google Scholar
- 3.Bennet, H. P. J., Browne, C. A., Goltzman, D., and Solomon S., 1979, Peptides: Structure and Biological Function (E. Gross and J. Meienhofer, eds.), Pierce Chemical Co., Rockford, Illinois, pp. 121–124.Google Scholar