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Selected Micromethods for Use in Neurochemistry

  • Volker Neuhoff

Abstract

Micromethods do not seem to be widely applied in neurochemical analysis, perhaps because of a widespread prejudice that micromethods are only useful for “microminded,” technically perfect experimenters. In addition, there may be worries about the reproducibility of microtechniques. A microgel, however, with many clearly separated and well-defined bands, each representing some nanograms of protein (see Fig. 10), is rather impressive when seen for the first time, especially when it is realized that the time taken for separation, staining, and destaining is only about one-tenth of the time necessary for the equivalent procedure using a macroscale method. A saving of experimental time is inherent in most of the micromethods described in some detail in this chapter. Such micromethods are also reproducible if they are performed correctly. The use of micromethods is therefore to be recommended even when the amount of material to be analyzed is not limited. Furthermore, now that it is harder to get sufficient funds for relevant research, micromethods are useful because the equipment needed for the analyses is not expensive.

Keywords

Sodium Dodecyl Sulfate Human Serum Albumin Protein Spot Dansyl Chloride Dansyl Amino Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Pun, J. Y., and Lombrozo, K., 1964, Anal Biochem. 9:9–20.PubMedCrossRefGoogle Scholar
  2. 2.
    Hydén, H., 1943, Acta Physiol. Scand. [Suppl.] 17(6):1–136.Google Scholar
  3. 3.
    Edstrøm, J.-E., 1953, Biochem. Biophys. Acta 12:361–386.PubMedCrossRefGoogle Scholar
  4. 4.
    Edstrøm, J.-E., and Hydén, H., 1954, Nature 174:128–129.PubMedCrossRefGoogle Scholar
  5. 5.
    Edstrøm, J.-E., 1960, J. Biophys. Biochem. Cytol. 8:39–43.PubMedCrossRefGoogle Scholar
  6. 6.
    Lindstrøm-Lang, K., 1937, Nature 140:108.CrossRefGoogle Scholar
  7. 7.
    Lowry, O. H., Roberts, N. R., and Chang, M. W., 1956, J. Biol. Chem. 222:97–107.PubMedGoogle Scholar
  8. 8.
    Giacobini, E., and Zajicek, J., 1956, Nature 177:185–186.PubMedCrossRefGoogle Scholar
  9. 9.
    Lowry, O. H., Passonneau, J. V., Schultz, D. W., and Rock, M. K., 1961, J. Biol. Chem. 236:2746–2755.PubMedGoogle Scholar
  10. 10.
    Giacobini, E., and Grasso, A., 1966, Acta Physiol. Scand. 66:49–57.PubMedCrossRefGoogle Scholar
  11. 11.
    Giacobini, E., and Marchisio, P. C, 1966, Acta Physiol. Scand. 66:247–248.PubMedCrossRefGoogle Scholar
  12. 12.
    Buckley, G., Consolo, S., Giacobini, E., and McCaman, R., 1967, Acta Physiol. Scand. 71:341–347.PubMedCrossRefGoogle Scholar
  13. 13.
    Brattgard, S.-O., and Hydén, H., 1952, Acta Radiol. [Suppl.] (Stockholm) 94:1–48.Google Scholar
  14. 14.
    Neuhoff, V., 1971, Anal. Biochem. 41:270–271.PubMedCrossRefGoogle Scholar
  15. 15.
    Carlsson, B., Giacobini, E., and Hovmark, S., 1967, Acta Physiol. Scand. 71:379–390.PubMedCrossRefGoogle Scholar
  16. 16.
    Haljamae, H., and Larsson, S., 1968, Chem. Instrum. 1:131–144.CrossRefGoogle Scholar
  17. 17.
    Edstrom, J.-E., and Neuhoff, V., 1973, Micromethods in Molecular Biology (V. Neuhoff, ed.), Springer-Verlag, Berlin, Heidelberg, New York, pp. 215–256.Google Scholar
  18. 18.
    Neuhoff, V., von der Haar, F., Schlimme, E., and Weise, M., 1969, Hoppe Seylers Z. Physiol. Chem. 350:121–128.PubMedCrossRefGoogle Scholar
  19. 19.
    Neuhoff, V., and Weise, M. 1970, Arzneim. Forsch. 20:368–372.Google Scholar
  20. 20.
    Briel, G., Neuhoff, V., and Osborn, N. N., 1971, Int. J. Neurosci. 2:129–136.PubMedCrossRefGoogle Scholar
  21. 21.
    Briel, G., and Neuhoff, V. 1972, Hoppe Seylers Z. Physiol. Chem. 353:540–553.PubMedCrossRefGoogle Scholar
  22. 22.
    Schiefer, H.-G., and Neuhoff, V., 1971, Hoppe Seylers Z. Physiol. Chem. 352:913–926.PubMedCrossRefGoogle Scholar
  23. 23.
    Althaus, H. H., and Neuhoff, V., 1973, Hoppe Seylers Z. Physiol. Chem. 354:1073–1976.PubMedCrossRefGoogle Scholar
  24. 24.
    Althaus, H. H., Osborne, N. N., and Neuhoff, V., 1973, Naturwissenschaften 60:553–554.PubMedCrossRefGoogle Scholar
  25. 25.
    Quentin, C.-D., and Neuhoff, V., 1972, Int. J. Neurosci. 4:17–24.PubMedCrossRefGoogle Scholar
  26. 26.
    Neuhoff, V., Ewers, E., and Huether, G., 1981, Hoppe Seylers Z. Physiol. Chem. 362:1427–1434.PubMedCrossRefGoogle Scholar
  27. 27.
    Seiler, P., Neuhoff, V., and Thorn, W., 1982, Hoppe Seylers Z. Physiol. Chem. (in press).Google Scholar
  28. 28.
    Neuhoff, V., 1973, Micromethods in Molecular Biology (V. Neuhoff, ed.), Springer-Verlag, Berlin. Heidelberg, New York, pp. 399–402.CrossRefGoogle Scholar
  29. 29.
    Osborn, N. N., 1974, Microchemical Analysis of Nervous Tissue. Pergamon Press, New York, Oxford.Google Scholar
  30. 30.
    Feigl, F., 1960, Tüpfelanalyse, II. Organischer Teil. Akademische Verlagsgesellschaft MBH, Frankfurt/Main.Google Scholar
  31. 31.
    Neuhoff, V., Philipp, K., Zimmer, H.-G., and Mesecke, S., 1979, Hoppe Seylers Z. Physiol. Chem. 369:1657–1670.CrossRefGoogle Scholar
  32. 32.
    Neuhoff, V., 1973, Micromethods in Molecular Biology, (V. Neuhoff, ed.), Springer-Verlag, Berlin, Heidelberg, New York, pp. 4–5.CrossRefGoogle Scholar
  33. 33.
    Zimmer, H.-G., Kiehl, F., and Neuhoff, V., 1979, Hoppe Seylers Z. Physiol. Chem. 360:1671–1672.PubMedCrossRefGoogle Scholar
  34. 34.
    Neuhoff, V., 1973, Micromethods in Molecular Biology, (V. Neuhoff, ed.), Springer-Verlag, Berlin, Heidelberg, New York, pp. 149–178.CrossRefGoogle Scholar
  35. 35.
    Norton, W. T., 1977, Myelin (P. Morell, ed.), Plenum Press, New York, pp. 161–199.Google Scholar
  36. 36.
    Lowry, O. H., Rosebrough, N. I., Farr, A. C, and Randall, R. I., 1951, J. Biol. Chem. 193:265–275.PubMedGoogle Scholar
  37. 37.
    Zimmer, H.-G., and Neuhoff, V., 1977, GIT Fachz. Lab. 21:104–105.Google Scholar
  38. 38.
    Neuhoff, V., 1973, Micromethods in Molecular Biology (V. Neuhoff, ed.), Springer-Verlag, Berlin, Heidelberg, New York.CrossRefGoogle Scholar
  39. 39.
    Marshall, R. D., and Neuberger, A., 1972, Glycoproteins, Volume 5, Part A (A. Gottschalk, ed.), Elsevier, Amsterdam, pp. 224–290.Google Scholar
  40. 40.
    Grossbach, U., 1965, Biochim. Biophys. Acta 107:180–182.PubMedCrossRefGoogle Scholar
  41. 41.
    Hyden, H., Bjurstam, K., and McEwen, B., 1966, Anal. Biochem. 17:1–15.PubMedCrossRefGoogle Scholar
  42. 42.
    McEwen, b., and Hydén, H., 1966, J. Neurochem. 13:823–833.PubMedCrossRefGoogle Scholar
  43. 43.
    Hydén, H., and Lange, P. W., 1968, J. Chromatogr. 35:336–351.PubMedCrossRefGoogle Scholar
  44. 44.
    Neuhoff, V., 1968, Arzneim. Forsch. 18:35–39.Google Scholar
  45. 45.
    Hydén, H., and Lange, P. W., 1972, Proc. Natl. Acad. Sci. U.S.A. 69:1980–1984.PubMedCrossRefGoogle Scholar
  46. 46.
    Griffith, A., and LaVelle, A., 1971, Exp. Neurol. 33:360–371.PubMedCrossRefGoogle Scholar
  47. 47.
    Ansorg, R., Dames, W., and Neuhoff, V., 1971, Arzneim. Forsch. 21:699–710.Google Scholar
  48. 48.
    Ansorg, R., and Neuhoff, V., 1971, Int. J. Neurosci. 2:151–160.PubMedCrossRefGoogle Scholar
  49. 49.
    Nir, I., Dames, W., and Neuhoff, V., 1973, Arch. Int. Physiol. Biochem. 81:607–616.CrossRefGoogle Scholar
  50. 50.
    Neuhoff, V., Schill, W.-B., and Sternbach, H., 1970, Biochem. J. 117:623–631.PubMedGoogle Scholar
  51. 51.
    Cupello, A., and Hydén, H., 1975, Neurobiology 5:129–136.PubMedGoogle Scholar
  52. 52.
    Rüchel, R., Mesecke, S., Wolfrum, D. I., and Neuhoff, V., 1973, Hoppe Seylers Z. Physiol. Chem. 354:1351–1368.PubMedCrossRefGoogle Scholar
  53. 53.
    Rüchel, R., Mesecke, S., Wolfrum, D. L, and Neuhoff, V., 1974, Hoppe Seylers Z. Physiol. Chem. 355:997–1020.CrossRefGoogle Scholar
  54. 54.
    Wolfrum, D. L, Rüchel, R., Mesecke, S., and Neuhoff, V., 1974, Hoppe Seylers Z. Physiol. Chem. 355:1415–1435.PubMedCrossRefGoogle Scholar
  55. 55.
    Rüchel, R., Richter-Landsberg, C, and Neuhoff, V., 1975, Hoppe Seylers Z. Physiol. Chem. 356:1283–1288.PubMedCrossRefGoogle Scholar
  56. 56.
    Endou, H., and Neuhoff, V., 1975, Hoppe Seylers Z. Physiol. Chem. 356:1381–1396.PubMedCrossRefGoogle Scholar
  57. 57.
    Reichel, W., Wolfrum, D., Weber, M., Scheler, F., and Neuhoff, V., 1975, Contrib. Nephrol. 1:109–118.PubMedGoogle Scholar
  58. 58.
    Poehling, H. M., Wolfrum, D. L, and Neuhoff, V., 1976, Entomologia Exp. Appl. 19:271–286.CrossRefGoogle Scholar
  59. 59.
    Peter, R., Wolfrum, D. L, and Neuhoff, V., 1976, Comp. Biochem. Physiol. 55B:583–589.Google Scholar
  60. 60.
    Fagg, G. E., Waehneldt, T. V., and Neuhoff, V., 1978, Myelination and Demyelination (J. Palo, ed.), Plenum Press, New York, pp. 135–145.CrossRefGoogle Scholar
  61. 61.
    Fagg, G. E., Schipper, H. L, and Neuhoff, V., 1979, Brain Res. 167:251–258.PubMedCrossRefGoogle Scholar
  62. 62.
    Tauber, H., Waehneldt, T. V., and Neuhoff, V., 1980, Neurosci. Lett. 16:235–238.PubMedCrossRefGoogle Scholar
  63. 63.
    Grossbach, U., 1972, Biochem. Biophys. Res. Commun. 49:667–672.PubMedCrossRefGoogle Scholar
  64. 64.
    Gainer, H., 1973, Anal. Biochem. 51:646–650.PubMedCrossRefGoogle Scholar
  65. 65.
    Bispink, G., and Neuhoff, V., 1976, Hoppe Seylers Z. Physiol. Chem. 357:991–997.PubMedCrossRefGoogle Scholar
  66. 66.
    Bispink, G., and Neuhoff, V., 1977, Electrofocusing and Isotachophoresis (R. J. Radola, and D. Graesslin, eds.), Walter de Gruyter, Berlin, pp. 135–146.Google Scholar
  67. 67.
    Gustke, H. H., and Neuhoff, V., 1978, Hoppe Seylers Z. Physiol. Chem. 359:1481–1489.PubMedCrossRefGoogle Scholar
  68. 68.
    Gustke, H. H., and Neuhoff, V., 1979, Hoppe Seylers Z. Physiol. Chem. 360:605–608.PubMedCrossRefGoogle Scholar
  69. 69.
    Neuhoff, V., and Poehling, H.-M., 1980, Hoppe Seylers Z. Physiol. Chem. 361:77–78.PubMedCrossRefGoogle Scholar
  70. 70.
    Neuhoff, V., and Schill, W.-B., 1968, Hoppe Seylers Z. Physiol. Chem. 349:795–800.PubMedCrossRefGoogle Scholar
  71. 71.
    Neuhoff, V., and Mesecke, S., 1977, Hoppe Seylers Z. Physiol. Chem. 358:1623–1637.PubMedCrossRefGoogle Scholar
  72. 72.
    Poehling, H.-M., and Neuhoff, V., 1980, Electrophoresis 1:90–102.CrossRefGoogle Scholar
  73. 73.
    Poehling, H.-M., Wyss, U., and Neuhoff, V., 1980, Electrophoresis 1:198–200.CrossRefGoogle Scholar
  74. 74.
    Huether, G., and Neuhoff, V., 1981, Histochem. J . 13:207–225.PubMedCrossRefGoogle Scholar
  75. 75.
    Sprinzel, M., Wolfrum, D. L, and Neuhoff, V., 1975, FEBS Lett. 50:54–56.CrossRefGoogle Scholar
  76. 76.
    Kolin, A., 1954, J. Chem. Phys. 22:1628–1629.CrossRefGoogle Scholar
  77. 77.
    Svensson, H., 1961, Acta Chem. Scand. 15:325–341.CrossRefGoogle Scholar
  78. 78.
    Vesterberg, O., and Svensson, H., 1966, Acta Chem. Scand. 20:820–834.PubMedCrossRefGoogle Scholar
  79. 79.
    Dale, G., and Latner, A., 1968, Lancet 1:847–848.PubMedCrossRefGoogle Scholar
  80. 80.
    Wrigley, C. W., 1968, J. Chromatogr. 36:362–365.PubMedCrossRefGoogle Scholar
  81. 81.
    Haglund, H., 1971, Methods in Biochemical Analysis (D. Glick, ed.), Volume 19, Wiley-Interscience, London, pp. 1–104.CrossRefGoogle Scholar
  82. 82.
    Riley, R. F., and Coleman, M. K., 1968, J. Lab. Clin. Med. 72:714–720.PubMedGoogle Scholar
  83. 83.
    Catsimpoolas, N., 1968, Anal. Biochem. 26:480–482.PubMedCrossRefGoogle Scholar
  84. 84.
    Baumann, J., and Chrambach, A., 1976, Anal. Biochem. 77:216–225.Google Scholar
  85. 85.
    Laemmly, U. K., 1970, Nature 227:680–685.CrossRefGoogle Scholar
  86. 86.
    O’Farrel, P. H., 1975, J. Biol. Chem. 250:4007–4021.Google Scholar
  87. 87.
    Klose, J., Blohm, M., and Gerner, L., 1977, Methods in Prenatal Toxicology (D. Neubert, H. J. Merker, and T. Kwasigroch, eds.), Georg Thieme, Stuttgart, PP. 303–313.Google Scholar
  88. 88.
    Radola, B. J., 1980, Electrophoresis 1:43–56.CrossRefGoogle Scholar
  89. 89.
    Norton, W. T., and Poduslo, S. E., 1973, J. Neurochem. 21:749–757.PubMedCrossRefGoogle Scholar
  90. 90.
    Lane, J. D., and Neuhoff, V., 1980, Naturwissenschaften 67:227–233.PubMedCrossRefGoogle Scholar
  91. 91.
    Lane, J. D., Schöne, B., Langenbeck, U., and Neuhoff, V., 1980, Biochim. Biophys. Acta 627:144–156.PubMedCrossRefGoogle Scholar
  92. 92.
    Huether, G., and Neuhoff, V., 1981, J. Int. Metab. Dis. 4:67–68.CrossRefGoogle Scholar
  93. 93.
    Görg, A., Postel, W., and Westermeier, R., 1978, Anal. Biochem. 89:60–70.PubMedCrossRefGoogle Scholar
  94. 94.
    Switzer, R. C., Merrill, C. R., and Shifrin, S., 1979, Anal. Biochem. 98:231–237.PubMedCrossRefGoogle Scholar
  95. 95.
    Allen, R. C., 1980, Electrophoresis 1:32–37.CrossRefGoogle Scholar
  96. 96.
    Merrill, C. R., Goldman, D., Sedman, S. A., and Ebert, M. H., 1981, Science 211:1437–1438.CrossRefGoogle Scholar
  97. 97.
    Poehing, H.-M., and Neuhoff, V., 1981, Electrophoresis 2:141–147.CrossRefGoogle Scholar
  98. 98.
    O’Farrell, P. H., 1975, J. Biol. Chem. 250:4007–4021.PubMedGoogle Scholar
  99. 99.
    Cremer, T., Dames, W., and Neuhoff, V., 1972, Hoppe Seylers Z. Physiol. Chem. 353:1317–1329.PubMedCrossRefGoogle Scholar
  100. 100.
    Gray, W. R., 1967, Methods Enzymol. 17:139–151.CrossRefGoogle Scholar
  101. 101.
    Seiler, N., 1970, Methods Biochem. Anal. 18:259–337.PubMedCrossRefGoogle Scholar
  102. 102.
    Seiler, N., and Wiechmann, M., 1970, Progress in Thin-Layer Chromatography and Related Methods (A. Niederwieser and G. Pataki, eds.), Volume III, Ann Arbor Science Publications, Ann Arbor, pp. 95–144.Google Scholar
  103. 103.
    Woods, K. R., and Wang, K. T., 1967, Biochim. Biophys. Acta 133:369–370.PubMedCrossRefGoogle Scholar
  104. 104.
    Wang, K. T., and Weinstein, B., 1972, Progress in Thin-Layer Chromatography and Related Methods (A. Niederwieser and G. Pataki, eds.), Volume III, Ann Arbor Science Publications, Ann Arbor, pp. 177–231.Google Scholar
  105. 105.
    Gray, W. R., and Hartley, B. S., 1963, Biochem. J. 89:59.Google Scholar
  106. 106.
    Neadle, D. J., and Pollit, R. J., 1965, Biochem. J. 97:607–608.PubMedGoogle Scholar
  107. 107.
    Neuhoff, V., 1973, Micromethods in Molecular Biology (V. Neuhoff, ed.), Springer Verlag, Berlin, Heidelberg, New York, pp. 85–148.CrossRefGoogle Scholar
  108. 108.
    Zimmer, H.-G., Neuhoff, V., and Schulze, E., 1976, J. Chromatogr. 124:120–122.CrossRefGoogle Scholar
  109. 109.
    Leonard, B. E., Neuhoff, V., and Tonge, S. R., 1974, Z. Naturforsch. 29c:184–186.Google Scholar
  110. 110.
    Osborne, N. N., Wu, P. H., and Neuhoff, V., 1974, Brain Res. 74:175–181.PubMedCrossRefGoogle Scholar
  111. 111.
    Osborne, N. N., and Neuhoff, V., 1974, Brain Res. 74:366–369.PubMedCrossRefGoogle Scholar
  112. 112.
    Quentin, C.-D., Behbehani, A. W., Schulte, F. J., and Neuhoff, V., 1974, Neuropediatrie 5:138–145.CrossRefGoogle Scholar
  113. 113.
    Behbehani, A. W., Quentin, C.-D., Schulte, F. J., and Neuhoff, V., 1974, Neuropediatrie 5:258–270.CrossRefGoogle Scholar
  114. 114.
    Quentin, C.-D., Behbehani, A. W., Schulte, F. J., and Neuhoff, V., 1974, Neuropediatrie 5:271–278.CrossRefGoogle Scholar
  115. 115.
    Neuhoff, V., Behbehani, A. W., Quentin, C.-D., and Prinz, H., 1974, Hoppe Seylers Z. Physiol. Chem. 355:891–894.PubMedCrossRefGoogle Scholar
  116. 116.
    Leonard, B. E., Neuhoff, V., and Tonge, S. R., 1974, Z. Naturforsch. 29c:767–772.Google Scholar
  117. 117.
    Osborne, N. N., and Neuhoff, V., 1974, Brain Res. 80:251–264.PubMedCrossRefGoogle Scholar
  118. 118.
    Behbehani, A. W., Quentin, C.-D., and Neuhoff, V., 1975, Neurobiology 5:52–59.PubMedGoogle Scholar
  119. 119.
    Neuhoff, V., Behbehani, A. W., Quentin, C.-D., and Briel, C., 1975, Neurobiology 5:254–261.PubMedGoogle Scholar
  120. 120.
    Richter-Landsberg, C., and Neuhoff, V., 1975, Naturwissenschaften 62:491.PubMedCrossRefGoogle Scholar
  121. 121.
    Leonard, B. E., Neuhoff, V., and Tonge, S. R., 1975, J. Neurosci. Res. 1:83–92.PubMedCrossRefGoogle Scholar
  122. 122.
    Schulze, E., and Neuhoff, V., 1976, Hoppe SeylersZ. Physiol. Chem. 357:593–600.CrossRefGoogle Scholar
  123. 124.
    Stenzel, K., and Neuhoff, V., 1976, J. Neurosci. Res. 2:1–9.PubMedCrossRefGoogle Scholar
  124. 125.
    Osborne, N. N., Stahl, W. L., and Neuhoff, V., 1976, J. Chromatogr. 123:212–215.PubMedCrossRefGoogle Scholar
  125. 126.
    Osborne, N. N., and Neuhoff, V., 1977, J. Chromatogr. 134:489–496.PubMedCrossRefGoogle Scholar
  126. 127.
    Poehling, H.-M., Wyss, U., and Neuhoff, V., 1980, Physiol. Plant Pathol. 16:59–61.CrossRefGoogle Scholar
  127. 128.
    Ulmar, G., and Neuhoff, V., 1980, Exp. Neurol. 69:99–109.PubMedCrossRefGoogle Scholar
  128. 129.
    Meyer, W., Poehling, H.-M., and Neuhoff, V., 1980, Comp. Biochem. Physiol. 67C:83–86.Google Scholar
  129. 130.
    Zimmer, H.-G., and Neuhoff, V., 1975, GIT Fachz. Lab. 19:481–484.Google Scholar
  130. 131.
    Lane, J. D., Zimmer, H.-G., and Neuhoff, V., 1979, Hoppe Sylers Z. Physiol. Chem. 360:1405–1408.CrossRefGoogle Scholar
  131. 132.
    Zimmer, H.-G., and Neuhoff, V., 1977, Informatik-Fachberichte (W. Brauer, ed.), Springer-Verlag, Berlin, Heidelberg, New York, pp. 12–20.Google Scholar
  132. 133.
    Kronberg, G., Zimmer, H-G., and Neuhoff, V., 1978, Fresenius Z. Anal. Chem. 290:133–134.CrossRefGoogle Scholar
  133. 134.
    Kronberg, H., Zimmer, H.-G., and Neuhoff, V., 1979, Microsc. Acta 82:223–228.PubMedGoogle Scholar
  134. 135.
    Zimmer, H.-G., 1979, J. Microsc. 116:365–372.PubMedCrossRefGoogle Scholar
  135. 136.
    Kronberg, H., Zimmer, H.-G., and Neuhoff, V., 1980, Electrophoresis 1:27–32.CrossRefGoogle Scholar
  136. 137.
    Zimmer, H.-G., and Neuhoff, V., 1981, Naturwissenschaften 68:464–470.PubMedCrossRefGoogle Scholar
  137. 138.
    Zimmer, H.-G., Kronberg, H., Berstein, R., and Neuhoff, V., 1981, Pattern Recogn. 13:79–82.CrossRefGoogle Scholar
  138. 139.
    Zimmer, H.-G., Kronberg, H., and Neuhoff, V., 1980, Microsc. Acta [Suppl.] 4:217–221.Google Scholar
  139. 140.
    Kronberg, H., and Neuhoff, V., 1978, Informatik-Fachberichte, Volume 17 (E. Triendle, ed.), Springer-Verlag, Berlin, Heidelberg, New York, pp. 334–337.Google Scholar
  140. 141.
    Giacobini, E., 1975, J. Neurosci. Res. 1:1–18.PubMedCrossRefGoogle Scholar
  141. 142.
    Giacobini, E., 1968, Neurosci. Res. 1:1–202.Google Scholar
  142. 143.
    Giacobini, E., 1977, Biochemistry of Characterised Neurons (N. N. Osborne, ed.), Pergamon Press, Oxford, New York, pp. 3–17.Google Scholar
  143. 144.
    Lowry, O. H., 1953, J. Histochem. Cytochem. 1:420–428.PubMedCrossRefGoogle Scholar
  144. 145.
    Lowry, O. H., and Passonneau, J. V., 1972, A Flexible System of Enzymatic Analysis, Academic Press, New York, pp. 236–249.Google Scholar
  145. 146.
    Lehrer, G. M., 1973, Micromethods in Molecular Biology (V. Neuhoff, ed.), Springer-Verlag, Berlin, Heidelberg, New York, pp. 285–296.Google Scholar
  146. 147.
    Bahr, G. F., 1973, Micromethods in Molecular Biology (V. Neuhoff, ed.), Springer-Verlag, Berlin, Heidelberg, New York, pp. 257–284.Google Scholar
  147. 148.
    Hydén, H., and Rosengren, B., 1962, Biochim. Biophys. Acta 60:638–640.PubMedCrossRefGoogle Scholar
  148. 149.
    Hydén, H., and Larsson, S., 1960, Proceedings Second International Symposium on X-Ray Microscopy and X-Ray Microanalysis, Elsevier, Amsterdam, pp. 51–55.Google Scholar
  149. 150.
    Giacobini, E., 1970, Biochem. Psychopharmacol. 2:9–64.Google Scholar
  150. 151.
    Giacobini, E., 1962, J. Neurochem. 9:169–177.PubMedCrossRefGoogle Scholar
  151. 152.
    Larsson, S., 1972, Anal. Biochem. 50:245–254.PubMedCrossRefGoogle Scholar
  152. 153.
    Hydén, H., 1959, Nature 4684:433–435.CrossRefGoogle Scholar
  153. 154.
    Cummins, J., and Hydén, H., 1962, Biochim. Biophys. Acta 60:271–283.PubMedCrossRefGoogle Scholar
  154. 155.
    Hydén, H., Lange, P. W., and Larsson, S., 1980, J. Neurol. Sci. 45:303–316.PubMedCrossRefGoogle Scholar
  155. 156.
    Dörmer, P., 1973, Micromethods in Molecular Biology (V. Neuhoff, ed.), Springer-Verlag, Berlin, Heidelberg, New York, pp. 347–394.Google Scholar
  156. 157.
    Hydén, H., and Larsson, S., 1956, J. Neurochem. 1:134–144.PubMedCrossRefGoogle Scholar
  157. 158.
    Zimmer, H. G., 1973, Micromethods in Molecular Biology (V. Neuhoff, ed.), Springer-Verlag, Berlin, Heidelberg, New York, pp. 297–328.Google Scholar
  158. 159.
    Ruch, F., and Lehmann, U., 1973, Micromethods in Molecular Biology (V. Neuhoff, ed.), Springer-Verlag, Berlin, Heidelberg, New York, pp. 329–346.Google Scholar
  159. 160.
    Galjaard, H., Niermeijer, M. F., Hahnemann, N., Mohr, J., and Sørensen, S. A., Clin. Genet. 5:368–377.Google Scholar
  160. 161.
    Galjaard, H., Hoogeveen, A., Keijzer, W., De Wit-Verbeek, E., and Flek-Noot, C., 1974, Histochem. J. 6:491–509.PubMedCrossRefGoogle Scholar
  161. 162.
    Jongkind, J. F., Ploem, J. S., Reuser, A. J. J., and Galjaard, H., 1974, Histochemistry 40:221–229.PubMedCrossRefGoogle Scholar
  162. 163.
    Galjaard, H., Hoogeveen, A., De Wit-Verbeek, Keijzer, W., and Reuser, A. J. J., 1975, Histochem. J. 7:499–501.CrossRefGoogle Scholar
  163. 164.
    Galjaard, H., Hoogeveen, A., Van Der Veer, A., and Kleyer, W. J., 1976, Excerpta Med. Int. Congr. Ser. 411:194–206.Google Scholar
  164. 165.
    Van Der Veer, E., Kleijer, W. J., de Josselin de Jong, J. E., and Galjaard, H., 1978, Hum. Genet. 40:285–292.PubMedCrossRefGoogle Scholar
  165. 166.
    Galjaard, H., 1979, Ann. Clin. Biochem. 16:343–353.PubMedGoogle Scholar
  166. 167.
    Galjaard, H., 1980, Trends in Enzyme Histochemistry and Cytochemistry, Excerpta Medica, Amsterdam, pp. 161–180.Google Scholar
  167. 168.
    Aitken, D. A., Kleijer, W. J., Niermeijer, M. F., Herbschieb-Voogt, E., and Galjaard, H., 1980, Clin. Genet. 17:293–298.PubMedCrossRefGoogle Scholar
  168. 169.
    Galjaard, H., 1980, Trends Biochem. Sci. 5:201–203.CrossRefGoogle Scholar
  169. 170.
    De Josselin de Jong, J. E., Jongkind, J. F., and Ywema, H. R., 1980, Anal. Biochem. 102:120–125.PubMedCrossRefGoogle Scholar
  170. 171.
    Galjaard, H., 1980, Genetic Metabolic Diseases. Early Diagnosis and Prenatal Analysis, Elsevier/North-Holland, Amsterdam.Google Scholar
  171. 172.
    Outlaw, W. H., Jr., 1980, Annu. Rev. Plant Physiol. 31:299–311.CrossRefGoogle Scholar
  172. 173.
    Hydén, H., and Rönnbäck, L., 1975, Brain Res. 100:615–628.PubMedCrossRefGoogle Scholar
  173. 174.
    Sternberger, L. A., 1979, Immunocytochemistry, John Wiley & Sons, New York.Google Scholar
  174. 175.
    Chang, J. Y., Brauer, D., and Wittmann-Liebold, B., 1978, FEBS Lett. 93:205–214.CrossRefGoogle Scholar
  175. 176.
    Neuhoff, V., 1973, Micromethods in Molecular Biology (V. Neuhoff, ed.), Springer-Verlag, Berlin, Heidelberg, New York, pp. 205–214.CrossRefGoogle Scholar
  176. 177.
    Neuhoff, V., and Rödel, E., 1973, Hoppe Sylers Z. Physiol. Chem. 354:1541–1549.CrossRefGoogle Scholar
  177. 178.
    Neuhoff, V., 1973, Micromethods in Molecular Biology (V. Neuhoff, ed.), Springer-Verlag, Berlin, Heidelberg, New York, pp. 395–398.CrossRefGoogle Scholar
  178. 179.
    Eichner, D., 1966, Experientia 22:620.PubMedCrossRefGoogle Scholar
  179. 180.
    Neuhoff, V., 1973, Micromethods in Molecular Biology (V. Neuhoff, ed.), Springer-Verlag, Berlin, Heidelberg, New York, pp. 407–409.CrossRefGoogle Scholar
  180. 181.
    Neuhoff, V., 1973, Micromethods in Molecular Biology (V. Neuhoff, ed.), Springer-Verlag, Berlin, Heidelberg, New York, pp. 179–204.CrossRefGoogle Scholar
  181. 182.
    Hydén, H., and Rönnbäck, L., 1978, J. Neurol. Sci. 39:241–246.PubMedCrossRefGoogle Scholar
  182. 183.
    Althaus, H. H., Hutter, W. B., and Neuhoff, V., 1977, Hoppe Sylers Z. Physiol. Chem. 358:773–775.Google Scholar
  183. 184.
    Althaus, H. H., Neuhoff, V., Huttner, W. B., Monzain, B., and Shahar, A., 1978, Hoppe Sylers Z. Physiol. Chem. 359:773–775.Google Scholar
  184. 185.
    Althaus, H. H., Gebicke-Härter, P., and Neuhoff, V., 1979, Naturwissenschaften 66:117.PubMedCrossRefGoogle Scholar
  185. 186.
    Huttner, W. B., Meyermann, R., Neuhoff, V., and Althaus, H. H., 1979, Brain Res. 171:225–237.PubMedCrossRefGoogle Scholar
  186. 187.
    Fewster, M. E., and Blackstone, S., 1975, Neurobiology 5:316–328.PubMedGoogle Scholar
  187. 188.
    Chao, S. W., and Rumsby, M. G., 1977, Brain Res. 124:347–351.PubMedCrossRefGoogle Scholar
  188. 189.
    Poduslo, S. E., 1978, Adv. Exp. Med. Biol. 100:71–94.PubMedCrossRefGoogle Scholar
  189. 190.
    Szuchet, S., Arnason, B. G. W., and Polak, P. E., 1978, Biophys. J. 21:51a.Google Scholar
  190. 191.
    Kennedy, P. G. E., and Lisak, R. P., 1980, Neurosci. Lett. 16:229–233.PubMedCrossRefGoogle Scholar
  191. 192.
    McCarthy, K. D., and de Vellis, J., 1980, J. Cell Biol. 85:890–902.PubMedCrossRefGoogle Scholar
  192. 193.
    Szuchet, S., Stefansson, K., Wollmann, R. L., Dawson, G., and Arnason, B. G. W., 1980, Brain Res. 200:151–164.PubMedCrossRefGoogle Scholar
  193. 194.
    Gebicke-Härter, P. J., Althaus, H. H., Schwartz, P., and Neuhoff, V., 1981, Dev. Brain Res. 1:497–518.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Volker Neuhoff
    • 1
  1. 1.Forschungsstelle NeurochemieMax-Planck-Institut für Experimentelle MedizinGöttingenFederal Republic of Germany

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