Perfusion of the Isolated Brain

  • David D. Gilboe

Abstract

Customarily, studies of brain metabolism and physiology have employed both in vitro methods utilizing simplified systems containing cerebral tissue, cells, or organelles and in vivo techniques employing the intact animal. Although a considerable amount of useful information has been obtained from studies with these in vivo and in vitro systems, a number of difficulties are inherent in such preparations. For example, the usual membrane barriers are disrupted during preparation of most in vitro systems; consequently, some enzymes are placed in an atypical milieu which may contain abnormal concentrations of activators, inhibitors, or substrates. Furthermore, the anoxia encountered during preparation of brain slices, homogenates, and the various cells and organelles introduces another serious disadvantage, since similar periods of anoxia are known to produce adverse effects and irreversible changes in the higher centers of the brain. Thus, one is confronted with the overwhelming task of correcting the data obtained from the in vitro system for artifacts induced by the preparation and relating the results to metabolism in the intact organ. These problems could be avoided entirely by the use of in vivo preparations were it not for physiological and metabolic interference from other tissues and the difficulty in controlling blood flow and composition.

Keywords

Nicotine Glutamine Neurol Methionine Histidine 

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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • David D. Gilboe
    • 1
  1. 1.Departments of Neurosurgery and PhysiologyUniversity of Wisconsin Center for Health SciencesMadison, WisconsinUSA

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