Fusion of Microbial Protoplasts: Problems and Perspectives

  • Lajos Alföldi
Part of the Basic Life Sciences book series


Induced fusion of protoplasts is a relatively new way of genetic manipulation in microbial systems. The individual steps of the manipulation are shown in the scheme of Figure 1. The rigid cell wall of bacteria, fungi, yeast, and algae (as well as of plant cells) can be removed without influencing the viability and integrity of the cell. The wall-less microorganism is surrounded by a plasma membrane, in hypertonic media it is spherical in shape, and is called a protoplast. The protoplasts, under appropriate conditions, do resynthesize cell wall material and revert to the original form with characteristic morphology.


Protoplast Fusion Aspergillus Nidulans Penicillium Chrysogenum Rigid Cell Wall Brewing Yeast 
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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Lajos Alföldi
    • 1
  1. 1.Biological Research CenterHungarian Academy of SciencesSzegedHungary

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