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Fusion of Microbial Protoplasts: Problems and Perspectives

  • Lajos Alföldi
Part of the Basic Life Sciences book series

Abstract

Induced fusion of protoplasts is a relatively new way of genetic manipulation in microbial systems. The individual steps of the manipulation are shown in the scheme of Figure 1. The rigid cell wall of bacteria, fungi, yeast, and algae (as well as of plant cells) can be removed without influencing the viability and integrity of the cell. The wall-less microorganism is surrounded by a plasma membrane, in hypertonic media it is spherical in shape, and is called a protoplast. The protoplasts, under appropriate conditions, do resynthesize cell wall material and revert to the original form with characteristic morphology.

Keywords

Protoplast Fusion Aspergillus Nidulans Penicillium Chrysogenum Rigid Cell Wall Brewing Yeast 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Lajos Alföldi
    • 1
  1. 1.Biological Research CenterHungarian Academy of SciencesSzegedHungary

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