Advertisement

Plasmids as Vectors for Gene Cloning: Past, Present, and Future Use

  • Donald Helinski
Part of the Basic Life Sciences book series

Abstract

The title of this conference in itself suggests the merging of several traditional disciplines of study (chemistry, biochemistry, microbiology, and genetics) with recent and dramatic developments in molecular genetics. The revolutionary breakthroughs of recombinant DNA research are providing unique opportunities for the genetic manipulation of microorganisms. One of the major objectives of this conference is to assemble scientists of diverse expertise to focus collectively on the problem of constructing microorganisms with new or greatly enhanced capacities to produce chemical substances of agricultural, industrial, and medical importance. For this purpose, it is necessary to consider the most effective means of merging our understanding of the physiological, biochemical, and ecological properties of microorganisms with the powerful technological developments that have occurred in molecular genetics. This paper will deal with plasmids, a key molecular ingredient for the genetic manipulation of microorganisms using recombinant DNA techniques.

Keywords

Gene Cloning Broad Host Range Conjugal Transfer Multicopy Plasmid Plasmid Replicon 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Marmur, J., R. Rownd, S. Falkow, L.S. Baron, C.L. Schildkraut and P. Doty: Proc. Natl. Acad. Sci. USA 47: 972, 1961.CrossRefGoogle Scholar
  2. 2.
    Wohleiter, J.A., S. Falkow, R.V. Citarella and L.S. Baron: J. Mol. Biol. 9: 576, 1964.CrossRefGoogle Scholar
  3. 3.
    DeWitt, W., and D.R. Helinski: J. Mol. Biol. 13: 692, 1965.CrossRefGoogle Scholar
  4. 4.
    Falkow, S., R.V. Citarella, J.A. Wolheiter and T. Watanabe: J. Mol. Biol. 17: 102, 1966.CrossRefGoogle Scholar
  5. 5.
    Rownd, R., R. Nakaya and A. Nakamura: J. Mol. Biol. 17: 376, 1966.CrossRefGoogle Scholar
  6. 6.
    Roth, T.F. and D.R. Helinski: Proc-Natl. Acad. Sci. USA 58: 650, 1967.CrossRefGoogle Scholar
  7. 7.
    Bazaral, M. and D.R. Helinski: Biochemistry 9: 399, 1970.CrossRefGoogle Scholar
  8. 8.
    Clewell, D.B.: J. Bacteriol. 110: 667, 1972.Google Scholar
  9. 9.
    Clewell, D.B. and D.R. Helinski: J. Bacteriol. 110: 1135, 1972.Google Scholar
  10. 10.
    Kahn, M., R. Kolter, C. Thomas, D. Figurski, R. Meyer, E. Remaut, and D.R. Helinski in “Methods in Enzymology,” S.P. Collowick and N.O. Kaplan, eds. Academic Press, 1979.Google Scholar
  11. 11.
    Vinograd, J. and J. Lebowitz: J. Gen. Physiol. 49: 103, 1966.CrossRefGoogle Scholar
  12. 12.
    Hickson, F.T., T.F. Roth and D.R. Helinski: Proc. Natl. Acad. Sci. USA 8: 1731, 1967.CrossRefGoogle Scholar
  13. 13.
    Cohen, S.N., A.C.Y. Chang and C.L. Hsu: Proc. Natl. Acad. Sci. USA 69: 2110, 1972.CrossRefGoogle Scholar
  14. 14.
    Smith, H.O., K.W. Wilcox: J. Mol. Biol. 51: 379, 1970.CrossRefGoogle Scholar
  15. 15.
    Kelly, T.J. Jr., and H.O. Smith: J. Mol. Biol. 51: 3939, 1970.CrossRefGoogle Scholar
  16. 16.
    Cohen, S.N., A.C.Y. Chang, H.W. Boyer and R. Helling: Proc. Natl. Acad. Sci. USA 70: 3240, 1973.CrossRefGoogle Scholar
  17. 17.
    Williams, B.G. and F.R. Blattner in “Genetic Engineering: Principles and Methods,” Vol. 2. J.K. Setlow and A. Hollaender, eds. Plenum Press, New York, 1980.Google Scholar
  18. 18.
    Barnes, W. in “Genetic Engineering: Principles and Methods,” Vol. 2, J.K. Setlow and A. Hollaender, eds. Plenum Press, New York, 1980.Google Scholar
  19. 19.
    Bolivar, F., R.L. Rodriguez, P.J. Greene, M.C. Betlach, H.L. Heyneker, H.W. Boyer, J.H. Crosa and S. Falkow: Gene. 2: 95–113, 1977.CrossRefGoogle Scholar
  20. 20.
    Backman, K.M. Betlach, H.W. Boyer, and S. Yanofsky: Cold Spring Harbor Symp. Quant. Biol. 43: 69–76, 1978.CrossRefGoogle Scholar
  21. 21.
    Tomizawa, J., Y. Sakakibara and T. Kakefoda: Proc. Natl. Acad. Sci. USA 72: 1050, 1975.CrossRefGoogle Scholar
  22. 22.
    Donoghue, D.J. and P.A. Sharp: J. Bacteriol. 133: 1287–94, 1978.Google Scholar
  23. 23.
    Kahn, M. and D.R. Helinski: Proc. Natl. Acad. Sci. USA 75: 2200–04, 1978.CrossRefGoogle Scholar
  24. 24.
    Inuzuka, M. and D.R. Helinski: Proc. Natl. Acad. Sci. USA 75: 5381–85, 1978.CrossRefGoogle Scholar
  25. 25.
    Kolter, R., M. Inuzuka and D.R. Helinski: Cell 15: 1199–1208, 1978.CrossRefGoogle Scholar
  26. 26.
    Kontomichalou, P., M. Mitani and R.C. Clowes: J. Bacteriol. 103: 33–44, 1970.Google Scholar
  27. 27.
    Lovett, M.L., R.B. Sparks and D.R. Helinski: Proc. Natl. Acad. Sci. USA 72: 2905, 1975.CrossRefGoogle Scholar
  28. 28.
    Crosa, J.H., L.K. Luttrop, and S. Falkow: J. Mol. Biol. 124: 443–468, 1978.CrossRefGoogle Scholar
  29. 29.
    Inuzuka, N., M. Inuzuka and D.R. Helinski: J. Biol. Chem. 255: 11071–74, 1980.Google Scholar
  30. 30.
    Crosa, J.H.: J. Biol. Chem. 255: 11075–77, 1980.Google Scholar
  31. 31.
    Kolter, R. and D.R. Helinski, submitted for publication.Google Scholar
  32. 32.
    Stalker, D.M., R. Kolter and D.R. Helinski: Proc. Natl. Acad. Sci. USA 76: 1150–54, 1979.CrossRefGoogle Scholar
  33. 33.
    Stalker, D.B., R. Kolter and D.R.Helinksi, submitted for publication.Google Scholar
  34. 34.
    Chang, A.C.Y. and S.N. Cohen: J. Bact. 134: 1141–56, 1978.Google Scholar
  35. 35.
    Uhlin, B.E., S. Molin, P. Gustayson and K. Norstrom: Gene. 6: 91–106, 1979.CrossRefGoogle Scholar
  36. 36.
    Barth, P.T. in “Plasmids of Medical, Environmental and Commercial Importance,” K.N. Timmis and A. Puhler, eds., Elsevier/North Holland, Amsterdam, pp. 399–410, 1979.Google Scholar
  37. 37.
    Bagdasarian, M. and K.N. Timmis in “Current Topics of Microbiology & Immunology,” W. Goebel and P. Hofschreider, eds., 1981 (in press).Google Scholar
  38. 38.
    Ditta, G., S. Stanfield, D. Corbin and D.R. Helinski: Proc. Natl. Acad. Sci. USA 77: 7347, 1980.CrossRefGoogle Scholar
  39. 39.
    Ehrlich, D.S.: Proc. Natl. Acad. Sci. USA 75: 1433, 1978.CrossRefGoogle Scholar
  40. 40.
    Dubnau, D., T. Gryczan, S. Contente and A.G. Shivakumar in “Genetic Engineering: Principles & Methods,” Vol. 2. J.K. Setlow & A. Hollaender, eds. Plenum Press, New York, 1980.Google Scholar
  41. 41.
    Hopwood, D.A., M.J. Bibb, J.M. Ward and J. Westpheling in “Plasmids of Medical, Environmental and Commercial Importance,” K.N. Timmis and A. Puhler, eds., Elsevier/North-Holland Biomedical Press, 1979.Google Scholar
  42. 42.
    Struhl, K., D.T. Stinchcomb, S. Scherer and R.W. Davis: Proc. Natl. Acad. Sci. USA, in press.Google Scholar
  43. 43.
    Hinnen, A., J.B. Hicks, C. Ilgen, and G.R. Fink in “Genetics of Industrial Microorganisms,” Amer. Soc. for Microbiology, Washington, D.C. (in press).Google Scholar
  44. 44.
    Struhl, K., D.T. Stinchcomb, S. Scherer and R.W. Davis: Proc. Natl. Acad. Sci. USA 76: 1035, 1979.CrossRefGoogle Scholar
  45. 45.
    Clark, L. and J. Carbon: Nature 287: 504, 1980.CrossRefGoogle Scholar
  46. 46.
    Stinchcomb, D.T., C. Mann, M. Thomas and R.W. Davis. J. of Supramolecular Structure and Cellular Biochemistry, Supplement 5, p. 377, 1981Google Scholar
  47. 47.
    Hershfield, V., H.W. Boyer, C. Yanofsky, M.A. Lovett and D.R. Helinski: Proc. Natl. Acad. Sci. USA 71: 3455–59, 1974.CrossRefGoogle Scholar
  48. 48.
    Bernard, H.-U. and D.R. Helinski. Genetic Engineering 2: (in press), 1980.Google Scholar
  49. 49.
    Goeddel, D.V., H.L. Heyneker, T. Hozumi, R. Arentzen, K. Itakura, D.G. Yansura, M.J. Ross, G. Miozzari, R. Crea, and P.H. Seeburg: Nature, 544–548, 1979.Google Scholar
  50. 50.
    Villa-Komaroff, L., A. Efstratindis, S. Broome, P. Lomedico, R. Tizard, S.P. Naber, W.L. Chick and W. Gilbert: Proc. Natl. Acad. Sci. USA 75: 3727–31, 1978.CrossRefGoogle Scholar
  51. 51.
    Charnay, P., M. Perricandet, F. Galibert, and P. Tiollais: Nucleic Acids Res. 5: 4479–94, 1978.CrossRefGoogle Scholar
  52. 52.
    Shafferman, A., R. Kolter, D.M. Stalker and D.R. Helinski, submitted for publication, 1981.Google Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Donald Helinski
    • 1
  1. 1.Department of BiologyUniversity of California, San DiegoLa JollaUSA

Personalised recommendations