Genetic Deregulation of Ethanol-Related Genes

  • David P. Clark
  • William Lorowitz
  • John E. CronanJr.
Part of the Basic Life Sciences book series


Wild-type Escherichia coli strains are unable to use ethanol as a carbon source. However, they do produce ethanol as a fermentation product under certain conditions of anaerobic growth. In the absence of nitrate, at acidic pH, and especially in the presence of high phosphate concentrations, E. coli ferments glucose mainly to lactic acid. However, at alkaline pH and in the absence of phosphate an equimolar mixture of acetate plus ethanol is the major product (11).


Nitrate Reductase Alcohol Dehydrogenase Allyl Alcohol Fatty Acid Derivative Temperature Sensitive Mutant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Casadaban, M. J. and S. N. Cohen. (1972) Lactose genes fused to exogeneous promotors in one step using a Mu-lac bacteriophage: In vivo probe for transcriptional control sequences. Proc. Natl. Acad. Sci. USA 76: 4530–4533 (1979).Google Scholar
  2. 2.
    Clark, D. P. Unpublished observations.Google Scholar
  3. 3.
    Clark, D. P. and J. P. Beard. Altered phospholipid composition in Mutants of Escherichia coli sensitive or resistant to organic solvents. J. Gen. Microbiol. 113: 267–274 (1979).CrossRefGoogle Scholar
  4. 4.
    Clark, D. and J. E. Cronan, Jr. Escherichia coli mutants with altered control of alcohol dehydrogenase and nitrate reductase. J. Bacteriol. 141: 177–183 (1980).Google Scholar
  5. 5.
    Clark, D. and J. E. Cronan, Jr. Acetaldehyde coenzyme A dehydrogenase of Escherichia coli. J. Bacteriol. 144: 179–184 (1980).Google Scholar
  6. 6.
    Lorowitz, W. and D. P. Clark. Mutants of Escherichia coli in the structural gene for alcohol dehydrogenase. Manuscript in preparation (1981).Google Scholar
  7. 7.
    Rando, R. R. (1972) In situ generation of irreversible enzyme inhibitors. Nature New Biology 237: 53.CrossRefGoogle Scholar
  8. 8.
    Rando, R. R. (1974) Allyl alcohol-induced irreversible inhibition of yeast alcohol dehydrogenase. Biochem. Pharmacol. 23: 2328–2331.Google Scholar
  9. 9.
    Rudolph, F. B., D. L. Purich and H. J. Fromm. (1968) Coenzyme A-linked aldehyde dehydrogenase from Escherichia coli. J. Biol. Chem. 243: 5539–5545.Google Scholar
  10. 10.
    Schmitt, B. (1975) Aldehyde dehydrogenase activity of a complex particle from E coli. Biochimie. 57: 1001–1004.Google Scholar
  11. 11.
    Sokatch, J. R. Bacterial physiology and metabolism. Academic Press, London. (1969).Google Scholar
  12. 12.
    Still, J. L. Alcohol enzyme of Bacterium coli. Biochem. J. 34: 1177–1182 (1940).Google Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • David P. Clark
    • 1
  • William Lorowitz
    • 1
  • John E. CronanJr.
    • 1
  1. 1.Department of MicrobiologyUniversity of IllinoisUrbanaUSA

Personalised recommendations